Open Access

ARTICLE

The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells

XIAOXU ZHANG^1,2, LIN ZHANG¹, LIN DU³, HUIYAN SUN⁴, XIA ZHAO², YANG SUN¹, WEI WANG^2,*, LISHENG WANG^1,3,*
1 Laboratory of Molecular Diagnosis and Regenerative Medicine, Medical Research Center, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China
2 Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China
3 School of Nursing, Jilin University, Changchun, 130000, China
4 Yanda Medical Research Institute, Hebei Yanda Hospital, Sanhe, 065201, China
* Corresponding Authors: WEI WANG. Email: ; LISHENG WANG. Email:
(This article belongs to this Special Issue: Expert Opinions and Future Trends on Stem Cells, Biomaterials and Growth Factors)

BIOCELL 2023, 47(2), 385-392. https://doi.org/10.32604/biocell.2023.025203

Received 27 June 2022; Accepted 30 August 2022; Issue published 18 November 2022

Abstract

Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. However, during in vitro expansion, MSCs are prone to aging, which largely limits their application. Prostaglandin E-2 (PGE-2) is a key effector secreted by MSCs to exert immunomodulatory effects. By screening the compound library for PGE-2 secretion, the antioxidant trolox was verified as a stimulator of MSCs to secrete PGE-2. The effect of antioxidant trolox on biological characteristics of MSCS, including aging, proliferation, and gene expression, was examined. The results demonstrated that trolox can resist aging, promote proliferation, and enhance PGE-2 secretion of MSCs without affecting their surface marker expression. Furthermore, trolox treatment up-regulates miR-17-92 clusters in MSCs and may contribute to its anti-aging effects. Thus, trolox addition might be beneficial for MSCs expansion and their application.

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Keywords

Mesenchymal stem cells (MSCs); Trolox; Antioxidation; Prostaglandin E-2

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