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MED4 gene positively affects preadipocyte differentiation in Chinese red steppe cattle

MINGHONG WEI1,#, CHENG XIAO1,2,#, JIAN WU1,3, LIHONG QIN1,3, HONGLIANG LIU1,3, YANG CAO1,*, YUMIN ZHAO1,3,*

1 Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100, China
2 Institute of Muscle Biology and Growth, Research Institute for Farm Animal Biology (FBN), Dummerstorf, 18196, Germany
3 Key Laboratory of Beef Cattle Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Gongzhuling, 136100, China

* Corresponding Authors: YANG CAO. Email: email; YUMIN ZHAO. Email: email
# These authors contributed equally to this work

(This article belongs to the Special Issue: Cellular Signal Transduction in Biological Activities)

BIOCELL 2023, 47(9), 2115-2123. https://doi.org/10.32604/biocell.2023.030364

Abstract

Background: The regulatory genes related to lipid metabolism affect the intramuscular fat (IMF) content and improve meat quality traits. Mediator Complex Subunit 4 (MED4), a vitamin D receptor protein, affects vitamin D, livestock growth, carcass traits, and triglyceride deposition. However, the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown. Methods: This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle. The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western blotting. Results: The MED4 gene had significantly high expression during preadipocyte differentiation (p < 0.05). Overexpression of the MED4 gene increased the expression of the PPARγ gene, a preadipocyte differentiation biomarker, improved cellular lipid droplets and triglycerides accumulation, and positively accelerated adipocyte maturation (p < 0.05). Interference of the MED4 gene can negatively regulate preadipocyte differentiation. Conclusion: This study showed that the MED4 gene may affect the preadipocyte differentiation and adipogenesis in Chinese Red Steppe cattle by regulating the PPARγ gene.

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APA Style
WEI, M., XIAO, C., WU, J., QIN, L., LIU, H. et al. (2023). <i>med4</i> gene positively affects preadipocyte differentiation in chinese red steppe cattle. BIOCELL, 47(9), 2115-2123. https://doi.org/10.32604/biocell.2023.030364
Vancouver Style
WEI M, XIAO C, WU J, QIN L, LIU H, CAO Y, et al. <i>med4</i> gene positively affects preadipocyte differentiation in chinese red steppe cattle. BIOCELL . 2023;47(9):2115-2123 https://doi.org/10.32604/biocell.2023.030364
IEEE Style
M. WEI et al., "<i>MED4</i> gene positively affects preadipocyte differentiation in Chinese red steppe cattle," BIOCELL , vol. 47, no. 9, pp. 2115-2123. 2023. https://doi.org/10.32604/biocell.2023.030364



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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