Table of Content

Open Access iconOpen Access

ARTICLE

BMP-7 Attenuates TGF-β1-Induced Fibronectin Secretion and Apoptosis of NRK-52E Cells by the Suppression of miRNA-21

Zhong Yu, Xu Zai-Chun, Han Wun-Lun, Zhu Yun-Yun

Department of Nephrology, Tongde Hospital of Zhejiang Province, Hangzhou, China

Oncology Research 2015, 23(4), 147-154. https://doi.org/10.3727/096504016X14519157902645

Abstract

Bone morphogenetic protein-7 (BMP-7) inhibited the pathogenesis of renal injury in response to a variety of stimuli. However, little is known about the molecular regulation and mechanism of endogenous BMP-7 and its renoprotective functions. This study examined the regulation of BMP-7 and its role in the fibronectin secretion and apoptosis of NRK-52E cells resulting from transforming growth factor-β1 (TGF-β1) in vitro. Results showed that TGF-β1 promoted factor-associated suicide (FAS), FAS ligand (FASL), fibronectin (FN), and miRNA-21 expression, while it downregulated phospho-Smad1 (pSmad1), pSmad5, and pSmad8 expressions in NRK-52E cells. In contrast, BMP-7 alleviated TGF-β1-induced cell apoptosis, inhibited TGF-β1-induced higher expression of miRNA-21 and FN, and enhanced TGF-β1-attenuated phosphorylation of Smad1, Smad5, and Smad8. Furthermore, a chemical inhibitor of miRNA-21 also negatively affected TGF-β1-induced apoptosis and FN secretion. On the other hand, overexpression of miRNA-21 counteracted the inhibitory effect of BMP-7 on TGF-β1-induced FN secretion and apoptosis. However, BMP-7 showed no effects on TGF-β1-induced FN secretion and apoptosis following knockdown of miRNA-21. Taken together, these findings demonstrated that BMP-7 might inhibit TGF-β1-induced FN secretion and apoptosis by the suppression of miRNA-21 in NRK-52E cells.

Keywords


Cite This Article

APA Style
Yu, Z., Zai-Chun, X., Wun-Lun, H., Yun-Yun, Z. (2015). BMP-7 attenuates tgf-β1-induced fibronectin secretion and apoptosis of NRK-52E cells by the suppression of mirna-21. Oncology Research, 23(4), 147-154. https://doi.org/10.3727/096504016X14519157902645
Vancouver Style
Yu Z, Zai-Chun X, Wun-Lun H, Yun-Yun Z. BMP-7 attenuates tgf-β1-induced fibronectin secretion and apoptosis of NRK-52E cells by the suppression of mirna-21. Oncol Res. 2015;23(4):147-154 https://doi.org/10.3727/096504016X14519157902645
IEEE Style
Z. Yu, X. Zai-Chun, H. Wun-Lun, and Z. Yun-Yun, “BMP-7 Attenuates TGF-β1-Induced Fibronectin Secretion and Apoptosis of NRK-52E Cells by the Suppression of miRNA-21,” Oncol. Res., vol. 23, no. 4, pp. 147-154, 2015. https://doi.org/10.3727/096504016X14519157902645



cc Copyright © 2015 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 146

    View

  • 70

    Download

  • 0

    Like

Share Link