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MicroRNA-21 Regulates the Proliferation, Differentiation, and Apoptosis of Human Renal Cell Carcinoma Cells by the mTOR-STAT3 Signaling Pathway

Tao Liang, Xiao-Yong Hu, Yong-Hui Li, Bin-Qiang Tian, Zuo-Wei Li, Qiang Fu

Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, P.R. China

Oncology Research 2016, 24(5), 371-380. https://doi.org/10.3727/096504016X14685034103356

Abstract

MicroRNA-21 (miRNA-21), a kind of short, noncoding RNAs, functioned as a tumor marker and was upregulated in renal cell carcinoma (RCC). However, the underlying mechanisms of miRNA-21 in RCC were uncertain. Therefore, the effects and mechanisms of miRNA-21 on the proliferation, differentiation, and apoptosis of cultured human RCC cells were further investigated in this study. After slicing miRNA-21 in RCC cells, the viability, mRNA expression of C/EBPα and PPARγ, caspase 3 activity, and protein expression of mTOR, STAT3, and pSTAT3 were determined. It was found that knockdown of miRNA-21 downregulated the optical density (OD) value of cells, inhibited mRNA expression of PPARγ and C/EBPα, and enhanced activity of caspase 3. Furthermore, protein expression of pSTAT3 was also decreased in the absence of miRNA-21. Notably, miRNA-21-changed proliferation, differentiation, and apoptosis of human RCC cells were partially regulated following the block of the mTOR-STAT3 signaling pathway by the mTOR inhibitor (XL388). It was indicated that miRNA-21 promoted proliferation and differentiation and decreased apoptosis of human RCC cells through the activation of the mTOR-STAT3 signaling pathway.

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Cite This Article

APA Style
Liang, T., Hu, X., Li, Y., Tian, B., Li, Z. et al. (2016). Microrna-21 regulates the proliferation, differentiation, and apoptosis of human renal cell carcinoma cells by the mtor-stat3 signaling pathway. Oncology Research, 24(5), 371-380. https://doi.org/10.3727/096504016X14685034103356
Vancouver Style
Liang T, Hu X, Li Y, Tian B, Li Z, Fu Q. Microrna-21 regulates the proliferation, differentiation, and apoptosis of human renal cell carcinoma cells by the mtor-stat3 signaling pathway. Oncol Res. 2016;24(5):371-380 https://doi.org/10.3727/096504016X14685034103356
IEEE Style
T. Liang, X. Hu, Y. Li, B. Tian, Z. Li, and Q. Fu "MicroRNA-21 Regulates the Proliferation, Differentiation, and Apoptosis of Human Renal Cell Carcinoma Cells by the mTOR-STAT3 Signaling Pathway," Oncol. Res., vol. 24, no. 5, pp. 371-380. 2016. https://doi.org/10.3727/096504016X14685034103356



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