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MicroRNA-200a Suppresses Cell Invasion and Migration by Directly Targeting GAB1 in Hepatocellular Carcinoma

Jianlin Wang*1, Wenjie Song*1, Weiwei Shen†1, Xisheng Yang*, Wei Sun*, Sshibin Qu*, Runze Shang*, Ben Ma*, Meng Pu*, Kaishan Tao*, Kefeng Dou*, Haimin Li*

* Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, P.R. China
† Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi, P.R. China

Oncology Research 2017, 25(1), 1-10. https://doi.org/10.3727/096504016X14685034103798

Abstract

MicroRNA-200a (miR-200a) is frequently downregulated in most cancer types and plays an important role in carcinogenesis and cancer progression. In this study, we determined that miR-200a was downregulated in hepatocellular carcinoma (HCC) tissues and cell lines, consistent with the results of our previous study. Because a previous study suggested that downregulation of miR-200a is correlated with HCC metastasis, we aimed to elucidate the mechanism underlying the role of miR-200a in metastasis in HCC. Here we observed that overexpression of miR-200a resulted in suppression of HCC metastatic ability, including HCC cell migration, invasion, and metastasis, in vitro and in vivo. Furthermore, bioinformatics and luciferase reporter assays indicated that GAB1 is a direct target of miR-200a. Inhibition of GAB1 resulted in substantially decreased cell invasion and migration similar to that observed with overexpression of miR-200a in HCC cell lines, whereas restoration of GAB1 partially rescued the inhibitory effects of miR-200a. Taken together, these data provide novel information for comprehending the tumor-suppressive role of miR-200a in HCC pathogenesis through inhibition of GAB1 translation.

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APA Style
Wang, J., Song, W., Shen, W., Yang, X., Sun, W. et al. (2017). Microrna-200a suppresses cell invasion and migration by directly targeting GAB1 in hepatocellular carcinoma. Oncology Research, 25(1), 1-10. https://doi.org/10.3727/096504016X14685034103798
Vancouver Style
Wang J, Song W, Shen W, Yang X, Sun W, Qu S, et al. Microrna-200a suppresses cell invasion and migration by directly targeting GAB1 in hepatocellular carcinoma. Oncol Res. 2017;25(1):1-10 https://doi.org/10.3727/096504016X14685034103798
IEEE Style
J. Wang et al., "MicroRNA-200a Suppresses Cell Invasion and Migration by Directly Targeting GAB1 in Hepatocellular Carcinoma," Oncol. Res., vol. 25, no. 1, pp. 1-10. 2017. https://doi.org/10.3727/096504016X14685034103798



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