Home / Journals / OR / Vol.25, No.1, 2017
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    ARTICLE

    MicroRNA-200a Suppresses Cell Invasion and Migration by Directly Targeting GAB1 in Hepatocellular Carcinoma

    Jianlin Wang*1, Wenjie Song*1, Weiwei Shen†1, Xisheng Yang*, Wei Sun*, Sshibin Qu*, Runze Shang*, Ben Ma*, Meng Pu*, Kaishan Tao*, Kefeng Dou*, Haimin Li*
    Oncology Research, Vol.25, No.1, pp. 1-10, 2017, DOI:10.3727/096504016X14685034103798
    Abstract MicroRNA-200a (miR-200a) is frequently downregulated in most cancer types and plays an important role in carcinogenesis and cancer progression. In this study, we determined that miR-200a was downregulated in hepatocellular carcinoma (HCC) tissues and cell lines, consistent with the results of our previous study. Because a previous study suggested that downregulation of miR-200a is correlated with HCC metastasis, we aimed to elucidate the mechanism underlying the role of miR-200a in metastasis in HCC. Here we observed that overexpression of miR-200a resulted in suppression of HCC metastatic ability, including HCC cell migration, invasion, and metastasis, in More >

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    ARTICLE

    Overexpression of Aristaless-Like Homeobox-4 Inhibits Proliferation, Invasion, and EMT in Hepatocellular Carcinoma Cells

    Yao Shi*, Xiaoke Sun, Xiafen He
    Oncology Research, Vol.25, No.1, pp. 11-18, 2017, DOI:10.3727/096504016X14685034103833
    Abstract Aristaless-like homeobox-4 (ALX4), a member of the Aristaless-like homeobox family, has been found to be involved in tumor cell proliferation, migration, and invasion. However, the role of ALX4 in hepatocellular carcinoma (HCC) remains largely unclear. Therefore, in this study we investigated the effects of ALX4 on HCC. The study results indicated that the expression of ALX4 was downregulated in HCC tissues and cell lines. Furthermore, we demonstrated that overexpression of ALX4 inhibited the proliferation, invasion, and epithelial–mesenchymal transition (EMT) in HCC cells. We also found that ALX4 had an inhibitory effect on the sonic hedgehog More >

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    ARTICLE

    Knockdown of Rap2B Inhibits the Proliferation and Invasion in Hepatocellular Carcinoma Cells

    Li Zhang*, Hong-bin Duan, Yun-sheng Yang*
    Oncology Research, Vol.25, No.1, pp. 19-27, 2017, DOI:10.3727/096504016X14685034103914
    Abstract Rap2B, a member of the Ras family of small GTP-binding proteins, was found to be highly expressed in various human tumors and plays an important role in the development of tumors. However, the function of Rap2B in hepatocellular carcinoma (HCC) remains unclear. Therefore, in this study, we investigated the biological functions of Rap2B in HCC and the potential underlying mechanisms. Our results indicated that Rap2B was highly expressed in HCC tissues and cell lines. Rap2B silencing obviously inhibited the proliferation, migration, and invasion of HCC cells, as well as attenuated xenografted tumor growth in vivo. More >

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    ARTICLE

    MicroRNA-377 Downregulates Bcl-xL and Increases Apoptosis in Hepatocellular Carcinoma Cells

    Hongyan Ge*, Di Zou, Yingshu Wang*, Hao Jiang*, Liping Wang
    Oncology Research, Vol.25, No.1, pp. 29-34, 2017, DOI:10.3727/096504016X14719078133168
    Abstract Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell lines and primary human HCC tissues. The decreased expression of miR-377 contributes to the upregulation of Bcl-xL expression by targeting its 3'-untranslated region (3'-UTR). Functionally, knockdown of miR-377 noticeably increased HCC cell growth and colony formation and inhibited apoptosis. In contrast, overexpression of miR-377 suppressed cell proliferation and increased apoptosis. This study provides new More >

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    ARTICLE

    The Biological Effects of Dickkopf1 on Small Cell Lung Cancer Cells and Bone Metastasis

    Hailin Pang1, Ningqiang Ma1, Mi Jiao, Weiwei Shen, Bo Xin, Tongfei Wang, Feng Zhang, Lili Liu, Helong Zhang
    Oncology Research, Vol.25, No.1, pp. 35-42, 2017, DOI:10.3727/096504016X14719078133249
    Abstract The bone is among the most common sites of metastasis in patients with lung cancer. Over 30%–40% of lung cancers can develop bone metastasis, and no effective therapeutic methods exist in clinic cases. Wnt/β-catenin signaling and Dickkopf1 (DKK1) play important roles in the progression of lung cancer, which preferentially metastasizes to the skeleton. However, the role of DKK1 in osteotropism of small cell lung cancer (SCLC) remains to be elucidated. This study aimed to define the role of DKK1 in SCLC bone metastasis and investigate the underlying mechanisms. Our results demonstrated that the expression level… More >

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    ARTICLE

    Inhibition of Beclin-1-Mediated Autophagy by MicroRNA-17-5p Enhanced the Radiosensitivity of Glioma Cells

    Weichen Hou*, Lei Song, Yang Zhao*, Qun Liu*, Shuyan Zhang
    Oncology Research, Vol.25, No.1, pp. 43-53, 2017, DOI:10.3727/096504016X14719078133285
    Abstract The role of miRNAs in the radiosensitivity of glioma cells and the underlying mechanism is still far from clear. In the present study, we detected six downregulated and seven upregulated miRNAs in the serum after radiotherapy compared with paired serum samples before radiotherapy via miRNA panel PCR. Among these, miR-17-5p was highly reduced (fold change = −4.21). Further, we validated the levels of miR-17-5p in all serum samples with qRT-PCR. In addition, statistical analysis suggested that a reduced miR-17-5P level was positively associated with advanced clinical stage of glioma, incidence of relapse, and tumor differentiation. More >

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    ARTICLE

    Tumor Necrosis Factor (TNF)-α-Induced Protein 8-like-2 (TIPE2) Inhibits Proliferation and Tumorigenesis in Breast Cancer Cells

    Ke Wang, Yu Ren, Yang Liu, Jian Zhang, Jian-jun He
    Oncology Research, Vol.25, No.1, pp. 55-63, 2017, DOI:10.3727/096504016X14719078133320
    Abstract Tumor necrosis factor-a (TNF-a)-induced protein 8-like-2 (TNFAIP8L2 or TIPE2), a member of the tumor necrosis TNFAIP8 family, was found to be involved in the development and progression of several tumors. However, to date, the role of TIPE2 in breast cancer is still unclear. Thus, the aim of this study is to explore the role of TIPE2 in breast cancer. Our results indicated that TIPE2 expression was significantly decreased in human breast cancer tissue and cell lines. Overexpression of TIPE2 inhibited the proliferation in vitro and tumor xenograft growth in vivo. TIPE2 also inhibited the migration/invasion More >

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    ARTICLE

    MicroRNA-142-5p Overexpression Inhibits Cell Growth and Induces Apoptosis by Regulating FOXO in Hepatocellular Carcinoma Cells

    Kexin Lou*1, Ning Chen†1, Zhihong Li*, Bei Zhang, Xiuli Wang, Ye Chen*, Haining Xu*, Dongwei Wang*, Hao Wang*
    Oncology Research, Vol.25, No.1, pp. 65-73, 2017, DOI:10.3727/096504016X14719078133366
    Abstract Abnormal expression of microRNA (miR)-142-5p has been reported in hepatocellular carcinoma (HCC). However, little information is available regarding the functional role of miR-142-5p in HCC. We aimed to explore the effects of miR-142-5p aberrant expression on HCC cell growth and cell apoptosis, as well as the underlying mechanism. Human HCC cell lines HepG2 and SMMC-7721 cells were transfected with miR- 142-5p mimic, inhibitor, or a corresponding negative control. Cell viability, cell cycle distribution, and cell apoptosis were then analyzed. In addition, protein expression of Forkhead box, class O (FOXO) 1 and 3, a Bcl-2-interacting mediator… More >

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    ARTICLE

    miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells

    Minglei Zhang*, Dapeng Wang, Tongtong Zhu*, Ruofeng Yin*
    Oncology Research, Vol.25, No.1, pp. 75-81, 2017, DOI:10.3727/096504016X14719078133401
    Abstract MicroRNAs (miRNAs) are small conserved RNAs regulating specific target genes in posttranscriptional levels. They have been involved in multiple processes of tumor progression, including cell proliferation. miR-214-5p (also miR-214*) is a newly identified miRNA, and its functions are largely unknown. In this study, we explore the role of miR-214-5p in the proliferation and invasion of human osteosarcoma (OS) cells. The results showed that miR-214-5p was sharply reduced in OS tissues and cell lines, compared with normal tissues and cell lines. In addition, the miR-214-5p mimic greatly increased the miR-214-5p level and significantly decreased the proliferation More >

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    ARTICLE

    RASSF4 Overexpression Inhibits the Proliferation, Invasion, EMT, and Wnt Signaling Pathway in Osteosarcoma Cells

    Minglei Zhang*, Dapeng Wang, Tongtong Zhu*, Ruofeng Yin*
    Oncology Research, Vol.25, No.1, pp. 83-91, 2017, DOI:10.3727/096504016X14719078133447
    Abstract RASSF4, a member of the RASSF family, is broadly expressed in normal tissues but often inactivated in human cancers. Despite various studies on RASSF4, its role in osteosarcoma remains unclear. Therefore, in this study, we investigated the effects of RASSF4 expression on osteosarcoma cells and explored the underlying mechanism. The results of our study showed that RASSF4 was lowly expressed in osteosarcoma tissues and cells. RASSF4 overexpression significantly inhibited proliferation, migration, and invasion as well as the EMT process in osteosarcoma cells. Meanwhile, we found that RASSF4 overexpression markedly decreased the protein expression of β-catenin, More >

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    ARTICLE

    Identification of Sensitivity Predictors of Neoadjuvant Chemotherapy for the Treatment of Adenocarcinoma of Gastroesophageal Junction

    Shoumiao Li*†, Baozhong Li, Jiaxiang Wang*, Da Zhang*, Zhiqiang Liu, Zhizhong Zhang, Wei Zhang, Yunjie Wang, Dongxiao Bai, Jianyun Guan, Yong Zhang
    Oncology Research, Vol.25, No.1, pp. 93-97, 2017, DOI:10.3727/096504016X14719078133564
    Abstract The identification of reliable predictors of chemotherapy sensitivity and early screening of adenocarcinoma of gastroesophageal junction (AGEJ) patients who are resistant to chemotherapy has become an important area of clinical and translational research. We aimed to investigate the predictive value of seven cancerassociated cellular proteins for neoadjuvant chemotherapy in AGEJ patients. Clinical data of 93 patients who received neoadjuvant chemotherapy for locally advanced AGEJ between June 2010 and December 2014 were reviewed. All patients were administered the combination regimen of S-1 and oxaliplatin (SOX). Expression of P-glycoprotein (P-gp), glutathione S-transferase-p (GST-π), topoisomerase II (topo II),… More >

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    ARTICLE

    Silencing of Armadillo Repeat-Containing Protein 8 (ARMc8) Inhibits TGF-β-Induced EMT in Bladder Carcinoma UMUC3 Cells

    Xuan Liang*, Qun-Li Men, Yong-wei Li, He-Cheng Li§, Tie Chong§, Zhao-lun Li§
    Oncology Research, Vol.25, No.1, pp. 99-105, 2017, DOI:10.3727/096504016X14719078133609
    Abstract Armadillo repeat-containing protein 8 (ARMc8) is a key factor in regulating cell migration, proliferation, tissue maintenance, and tumorigenesis. However, its role in bladder cancer remains unknown. Thus, in this study we sought to investigate the effect of ARMc8 on the epithelial-to-mesenchymal transition (EMT) progress in bladder cancer cells induced by transforming growth factor-b1 (TGF-β1). Our results found that ARMc8 was highly expressed in bladder cancer cell lines. ARMc8 silencing inhibited the TGF-β1-induced migration and invasion and suppressed the EMT progress in bladder cancer cells. Furthermore, ARMc8 silencing inhibited the TGF-β1-induced expression of β-catenin, cyclin D1, More >

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    ARTICLE

    miR-940 Upregulation Suppresses Cell Proliferation and Induces Apoptosis by Targeting PKC-δ in Ovarian Cancer OVCAR3 Cells

    Fang Wang, Zhihong Wang, Xiaoli Gu, Jinquan Cui
    Oncology Research, Vol.25, No.1, pp. 107-114, 2017, DOI:10.3727/096504016X14732772150145
    Abstract Ovarian cancer remains as one of the most threatening malignancies for females in the world. This study investigated the pivotal role of miR-940 in the progression of ovarian cancer and to reveal the possible molecular mechanism of its action. Ovarian cancer OVCAR3 cells were transfected with the miR-940 vector, miR-940 inhibitor, and/or small interfering RNA (siRNA) targeting PKC-d (si-PKC-δ), respectively. After transfection, cell viability and cell apoptosis were analyzed, as well as cell proliferation and apoptosis-related protein expression. Compared to the control, miR-940 upregulation suppressed cell viability but induced cell apoptosis. miR- 940 upregulation increased… More >

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    ARTICLE

    Knockdown of Tripartite Motif-Containing Protein 37 (TRIM37) Inhibits the Proliferation and Tumorigenesis in Colorectal Cancer Cells

    Ping Zhao*, Hai-Tao Guan, Zhi-Jun Dai, Yu-Guang Ma, Xiao-Xu Liu, Xi-Jing Wang
    Oncology Research, Vol.25, No.1, pp. 115-122, 2017, DOI:10.3727/096504016X14732772150181
    Abstract Tripartite motif-containing protein 37 (TRIM37), a new member of the RING-B-box-coiled-coil (RBCC) subfamily of zinc finger proteins, was found to be involved in the development and progression of several cancers. However, the expression pattern and biological functions of TRIM37 in colorectal cancer (CRC) remain unknown. Therefore, in the present study, we examined the expression pattern of TRIM37 in CRC and investigated the function of TRIM37 in the progression of CRC. Our results showed that TRIM37 expression was upregulated in CRC cell lines. Knockdown of TRIM37 inhibited CRC cell proliferation and tumor growth in vivo. Furthermore, More >

  • Open AccessOpen Access

    ARTICLE

    Depletion of NFBD1/MDC1 Induces Apoptosis in Nasopharyngeal Carcinoma Cells Through the p53–ROS–Mitochondrial Pathway

    Zhihai Wang*, Kui Liao, Wenqi Zuo*, Xueliang Liu*, Zhili Qiu*, Zhitao Gong*, Chuan Liu*, Quan Zeng*, Yi Qian*, Liang Jiang*, Youquan Bu, Suling Hong*, Guohua Hu*
    Oncology Research, Vol.25, No.1, pp. 123-136, 2017, DOI:10.3727/096504016X14732772150226
    Abstract NFBD1, a signal amplifier of the p53 pathway, is vital for protecting cells from p53-mediated apoptosis and the early phase of DNA damage response under normal culture conditions. Here we investigated its expression in patients with nasopharyngeal carcinoma (NPC), and we describe the biological functions of the NFBD1 gene. We found that NFBD1 mRNA and protein were more highly expressed in NPC tissues than in nontumorous tissues. To investigate the function of NFBD1, we created NFBD1-depleted NPC cell lines that exhibited decreased cellular proliferation and colony formation, an increase in their rate of apoptosis, and More >

  • Open AccessOpen Access

    ARTICLE

    MicroRNA-92a Promotes Cell Proliferation in Cervical Cancer via Inhibiting p21 Expression and Promoting Cell Cycle Progression

    Zhiying Su*1, Hua Yang†1, Min Zhao*,‡ Yanlong Wang*, Guoyi Deng*, Ruixin Chen*
    Oncology Research, Vol.25, No.1, pp. 137-145, 2017, DOI:10.3727/096504016X14732772150262
    Abstract MicroRNA-92a (miR-92a) generally plays a promoting role in human cancers, but the underlying mechanism in cervical cancer remains unclear. Here we studied the expression and clinical significance of miR-92a in cervical cancer, as well as the regulatory mechanism in the proliferation of cervical cancer cells. Our data indicated that miR-92a was significantly upregulated in cervical cancer tissues compared to their matched adjacent nontumor tissues (ANTs), and the increased miR-92a levels were significantly associated with a higher grade, lymph node metastasis, and advanced clinical stage in cervical cancer. In vitro study revealed that inhibition of miR-92a… More >

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    ARTICLE

    Overexpression of MicroRNA-27b Inhibits Proliferation, Migration, and Invasion via Suppression of MET Expression

    Hui Zhou*†, Yanglin Liu, Ling Xiao, Zhengmao Hu*, Kun Xia
    Oncology Research, Vol.25, No.1, pp. 147-154, 2017, DOI:10.3727/096504016X14732772150505
    Abstract MicroRNA-27b (miR-27b) was recently found to be significantly downregulated in different human cancers. However, evidence of the function of miR-27b in non-small cell lung cancer (NSCLC) remains limited. In this study, we aimed to investigate novel miR-27b-mediated targets or signaling pathways associated with the tumorigenesis and metastasis of NSCLC. Real-time (RT) PCR was performed to examine miR-27b expression in NSCLC specimens. MTT assay, wound-healing assay, and Transwell assay were used to determine cell proliferation, migration, and invasion. Our data indicated that the miR-27b levels were significantly decreased in NSCLC specimens and cell lines (SK-MES-1, H358,… More >

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    ERRATUM

    Suppressive Role of MicroRNA-148a in Cell Proliferation and Invasion in Ovarian Cancer Through Targeting Transforming Growth Factor-β-Induced 2

    Min Zhao*1, Zhiying Su†1, Shiyang Zhang, Liangjin Zhuang§, Yudi Xie*, Xiaodong Li*
    Oncology Research, Vol.25, No.1, pp. 155-155, 2017, DOI:10.3727/096504017X14811155525280
    Abstract Ovarian cancer (OC) is one of the most common gynecological malignancies. MicroRNAs (miRs) play a crucial role in the development and progression of OC, but the underlying mechanism remains largely unclear. Our study investigated the regulatory role of miR-148a in OC cell proliferation and invasion. We found that miR- 148a was significantly downregulated in OC tissues compared to their matched adjacent nontumor tissues. In addition, its expression was also reduced in OC cell lines (SKOV3, ES-2, OVCAR, and A2780) compared to normal ovarian epithelial cells. Overexpression of miR-148a caused a significant decrease in OC cell… More >

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