Home / Journals / OR / Vol.29, No.5, 2021
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  • Open AccessOpen Access

    ARTICLE

    Computational high-throughput screening and in vitro approaches identify CB-006-3; A novel PI3K-BRAFV600E dual targeted inhibitor against melanoma

    FAISAL HASSAN TOBEIGEI1, REEM M. GAHTANI2, AHMAD SHAIKH2, AMER AL ALI3, NADER KAMELI4,5, HOSSAM KAMLI2, PRASANNA RAJAGOPALAN2,6,*
    Oncology Research, Vol.29, No.5, pp. 305-318, 2021, DOI:10.32604/or.2022.025187
    Abstract Malignant melanoma is characterized by both genetic and molecular alterations that activate phosphoinositide 3-kinase (PI3K), and RAS/BRAF pathways. In this work, through diversity-based high-throughput virtual screening we identified a lead molecule that selectively targets PI3K and BRAFV600E kinases. Computational screening, Molecular dynamics simulation and MMPBSA calculations were performed. PI3K and BRAFV600E kinase inhibition was done. A375 and G-361 cells were used for in vitro cellular analysis to determine antiproliferative effects, annexin V binding, nuclear fragmentation and cell cycle analysis. Computational screening of small molecules indicates compound CB-006-3 selectively targets PI3KCG (gamma subunit), PI3KCD (delta subunit) and BRAFV600E. Molecular dynamics simulation… More >

  • Open AccessOpen Access

    ARTICLE

    Biological and molecular studies on specific immune cells treated with checkpoint inhibitors for the thera-personal approach of breast cancer patients (ex-vivo study)

    MOTAWA E. EL-HOUSEINI1, MOSTAFA S. ARAFAT2, AHMED M. EL-HUSSEINY3, ISLAM M. KASEM2, MAHMOUD M. KAMEL4, AHMED H. EL-HABASHY5, MEDHAT M. KHAFAGY6, ENAS M. RADWAN4, MAHA H. HELAL7, MONA S. ABDELLATEIF1,*
    Oncology Research, Vol.29, No.5, pp. 319-329, 2021, DOI:10.32604/or.2022.025249
    Abstract Immunotherapy becomes a promising line of treatment for breast cancer (BC) however, its success rate is still limited. Methods: The study was designed to optimize the condition for producing an effective dendritic cell (DCs) based immunotherapy by using DCs and T lymphocytes together with tumor-infiltrating lymphocytes (TILs) and tumor-infiltrating DCs (TIDCs), treated with anti-PD1 and anti-CTLA4 monoclonal antibodies. This mixture of immune cells was co-cultured with autologous breast cancer cells (BCCs) isolated from 26 BC females. Results: There was a significant upregulation of CD86 and CD83 on DCs (P = 0.001 and 0.017, respectively), similarly upregulation of CD8, CD4 and… More >

  • Open AccessOpen Access

    ARTICLE

    Macrophage-derived exosomal miR-342-3p promotes the progression of renal cell carcinoma through the NEDD4L/CEP55 axis

    JIAFU FENG1,2,#,*, BEI XU1,2,#, CHUNMEI DAI1,2,#, YAODONG WANG1,4, GANG XIE1,5, WENYU YANG1,2, BIN ZHANG1,2, XIAOHAN LI6, JUN WANG3
    Oncology Research, Vol.29, No.5, pp. 331-349, 2021, DOI:10.32604/or.2022.03554
    Abstract Due to its difficulty in early diagnosis and lack of sensitivity to chemotherapy and radiotherapy, renal cell carcinoma (RCC) remains to be a frequent cause of cancer-related death. Here, we probed into new targets for its early diagnosis and treatment for RCC. microRNA (miRNA) data of M2-EVs and RCC were searched on the Gene Expression Omnibus database, followed by the prediction of the potential downstream target. Expression of target genes was measured via RT-qPCR and Western blot, respectively. M2 macrophage was obtained via flow cytometry with M2-EVs extracted. The binding ability of miR-342-3p to NEDD4L and to CEP55 ubiquitination was… More >

  • Open AccessOpen Access

    REVIEW

    Regulation of pathological blood-brain barrier for intracranial enhanced drug delivery and anti-glioblastoma therapeutics

    KAI WANG2,#, FENGTIAN ZHANG1,3,4,#, CHANGLONG WEN5, ZHIHUA HUANG6, ZHIHAO HU1, YUWEN ZHANG1, FUQIANG HU2,*, LIJUAN WEN1,6,*
    Oncology Research, Vol.29, No.5, pp. 351-363, 2021, DOI:10.32604/or.2022.025696
    Abstract The blood-brain barrier (BBB) is an essential component in regulating and maintaining the homeostatic microenvironment of the central nervous system (CNS). During the occurrence and development of glioblastoma (GBM), BBB is pathologically destroyed with a marked increase in permeability. Due to the obstruction of the BBB, current strategies for GBM therapeutics still obtain a meager success rate and may lead to systemic toxicity. Moreover, chemotherapy could promote pathological BBB functional restoration, which results in significantly reduced intracerebral transport of therapeutic agents during multiple administrations of GBM and the eventual failure of GBM chemotherapy. The effective delivery of intracerebral drugs still… More >

  • Open AccessOpen Access

    REVIEW

    Improvement method for cervical cancer detection: A comparative analysis

    NUR AIN ALIAS1, WAN AZANI MUSTAFA1,2,*, MOHD AMINUDIN JAMLOS3, AHMED ALKHAYYAT4, KHAIRUL SHAKIR AB RAHMAN5, RAMI Q. MALIK6
    Oncology Research, Vol.29, No.5, pp. 365-376, 2021, DOI:10.32604/or.2022.025897
    Abstract Cervical cancer is a prevalent and deadly cancer that affects women all over the world. It affects about 0.5 million women anually and results in over 0.3 million fatalities. Diagnosis of this cancer was previously done manually, which could result in false positives or negatives. The researchers are still contemplating how to detect cervical cancer automatically and how to evaluate Pap smear images. Hence, this paper has reviewed several detection methods from the previous researches that has been done before. This paper reviews pre-processing, detection method framework for nucleus detection, and analysis performance of the method selected. There are four… More >

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