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MicroRNA-92a Promotes Cell Proliferation in Cervical Cancer via Inhibiting p21 Expression and Promoting Cell Cycle Progression

Zhiying Su*1, Hua Yang†1, Min Zhao*,‡ Yanlong Wang*, Guoyi Deng*, Ruixin Chen*

* Department of Gynecology, Xiamen Maternal and Child Health Care Hospital, Xiamen, Fujian, P.R. China
† Department of Obstetrics and Gynecology VIP, Xiamen Maternal and Child Health Care Hospital, Xiamen, Fujian, P.R. China
‡ Department of Gynecology and Obstetrics, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, P.R. China
1 These authors provided equal contribution to this work.

Oncology Research 2017, 25(1), 137-145. https://doi.org/10.3727/096504016X14732772150262

Abstract

MicroRNA-92a (miR-92a) generally plays a promoting role in human cancers, but the underlying mechanism in cervical cancer remains unclear. Here we studied the expression and clinical significance of miR-92a in cervical cancer, as well as the regulatory mechanism in the proliferation of cervical cancer cells. Our data indicated that miR-92a was significantly upregulated in cervical cancer tissues compared to their matched adjacent nontumor tissues (ANTs), and the increased miR-92a levels were significantly associated with a higher grade, lymph node metastasis, and advanced clinical stage in cervical cancer. In vitro study revealed that inhibition of miR-92a led to a significant reduction in the proliferation of HeLa cells via induction of cell cycle arrest at the G1 stage. In contrast, overexpression of miR-92a markedly promoted the proliferation of HeLa cells by promoting cell cycle progression. Further investigation revealed that miR-92a has a negative effect on protein levels, but not the mRNA levels, of p21 in HeLa cells, suggesting that p21 is a direct target of miR-92a. Overexpression of p21 eliminated the promoting effects of miR-92a on the proliferation and cell cycle progression of HeLa cells. However, knockdown of p21 reversed the suppressive effects of miR-92a downregulation on HeLa cell proliferation and cell cycle progression. Moreover, p21 was significantly downregulated in cervical cancer tissues compared to ANTs, suggesting that the increased expression of miR-92a may contribute to the decreased expression of p21, which further promotes cervical cancer growth. In conclusion, our study demonstrates that miR-92a promotes the proliferation of cervical cancer cells via inhibiting p21 expression and promoting cell cycle progression, highlighting the clinical significance of miR-92a in cervical cancer.

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APA Style
Su, Z., Yang, H., Zhao, M., Wang, ‡.Y., Deng, G. et al. (2017). Microrna-92a promotes cell proliferation in cervical cancer via inhibiting p21 expression and promoting cell cycle progression. Oncology Research, 25(1), 137-145. https://doi.org/10.3727/096504016X14732772150262
Vancouver Style
Su Z, Yang H, Zhao M, Wang ‡Y, Deng G, Chen R. Microrna-92a promotes cell proliferation in cervical cancer via inhibiting p21 expression and promoting cell cycle progression. Oncol Res. 2017;25(1):137-145 https://doi.org/10.3727/096504016X14732772150262
IEEE Style
Z. Su, H. Yang, M. Zhao, ‡.Y. Wang, G. Deng, and R. Chen "MicroRNA-92a Promotes Cell Proliferation in Cervical Cancer via Inhibiting p21 Expression and Promoting Cell Cycle Progression," Oncol. Res., vol. 25, no. 1, pp. 137-145. 2017. https://doi.org/10.3727/096504016X14732772150262



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