Table of Content

Open Access iconOpen Access

ARTICLE

miR-1290 Contributes to Colorectal Cancer Cell Proliferation by Targeting INPP4B

Qingzhu Ma*, Yan Wang, Hualing Zhang*, Fengqiang Wang

* Department of Gastroenterology, Liaocheng People’s Hospital, Liaocheng, P.R. China
† Department of Respiratory Medicine, Liaocheng People’s Hospital, Liaocheng, P.R. China

Oncology Research 2018, 26(8), 1167-1174. https://doi.org/10.3727/096504017X15051741798389

Abstract

Colorectal cancer (CRC) is one of the most common oncological conditions worldwide, to date. MicroRNA- 1290 (miR-1290) has been demonstrated to regulate its progression. We studied the role of miR-1290 in CRC progression. The gene was upregulated in CRC tissues and cells. Its overexpression promoted CRC cell proliferation analyzed by MTT assay, colony formation assay, and soft agar growth assay. In addition, miR-1290 knockdown inhibited CRC cell proliferation. We also found that miR-1290 overexpression reduced the p27 level and increased cyclin D1 at both the mRNA and protein levels, whereas miR-1290 knockdown increased p27 and reduced cyclin D1, confirming miR-1290 promoted CRC cell proliferation. Inositol polyphosphate 4-phosphatase B (INPP4B) was the target of miR-1290. Luciferase reporter assay revealed that miR-1290 directly bound to the 3'-UTR of INPP4B; the mutated seed sites in miR-1290 abrogated this effect. Double knockdown of INPP4B and miR-1290 promoted CRC cell proliferation, suggesting miR-1290 promoted CRC cell proliferation by targeting INPP4B.

Keywords


Cite This Article

APA Style
Ma, Q., Wang, Y., Zhang, H., Wang, F. (2018). Mir-1290 contributes to colorectal cancer cell proliferation by targeting INPP4B. Oncology Research, 26(8), 1167-1174. https://doi.org/10.3727/096504017X15051741798389
Vancouver Style
Ma Q, Wang Y, Zhang H, Wang F. Mir-1290 contributes to colorectal cancer cell proliferation by targeting INPP4B. Oncol Res. 2018;26(8):1167-1174 https://doi.org/10.3727/096504017X15051741798389
IEEE Style
Q. Ma, Y. Wang, H. Zhang, and F. Wang, “miR-1290 Contributes to Colorectal Cancer Cell Proliferation by Targeting INPP4B,” Oncol. Res., vol. 26, no. 8, pp. 1167-1174, 2018. https://doi.org/10.3727/096504017X15051741798389



cc Copyright © 2018 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 169

    View

  • 113

    Download

  • 0

    Like

Share Link