Table of Content

Open Access iconOpen Access

ARTICLE

Propofol Inhibits Lung Cancer A549 Cell Growth and Epithelial–Mesenchymal Transition Process by Upregulation of MicroRNA-1284

Wei-Zhen Liu, Nian Liu

Department of Anesthesia, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, P.R. China

Oncology Research 2019, 27(1), 1-8. https://doi.org/10.3727/096504018X15172738893959

Abstract

Propofol has been widely used in lung cancer resections. Some studies have demonstrated that the effects of propofol might be mediated by microRNAs (miRNAs). This study aimed to investigate the effects and mechanisms of propofol on lung cancer cells by regulation of miR-1284. A549 cells were treated with different concentrations of propofol, while transfected with miR-1284 inhibitor, si-FOXM1, and their negative controls. Cell viability, migration, and invasion, and the expression of miR-1284, FOXM1, and epithelial–mesenchymal transition (EMT) factors were detected by CCK-8, Transwell, qRT-PCR, and Western blot assays, respectively. In addition, the regulatory and binding relationships among propofol, miR-1284, and FOXM1 were assessed, respectively. Results showed that propofol suppressed A549 cell viability, migration, and invasion, upregulated E-cadherin, and downregulated N-cadherin, vimentin, and Snail expressions. Moreover, propofol significantly promoted the expression of miR-1284. miR-1284 suppression abolished propofol-induced decreases of cell viability, migration, and invasion, and increased FOXM1 expression and the luciferase activity of FOXM1-wt. Further, miR-1284 negatively regulated FOXM1 expression. FOXM1 knockdown reduced cell viability, migration, and invasion by propofol treatment plus miR-1284 suppression. In conclusion, our study indicated that propofol could inhibit cell viability, migration, invasion, and the EMT process in lung cancer cells by regulation of miR-1284.

Keywords


Cite This Article

APA Style
Liu, W., Liu, N. (2019). Propofol inhibits lung cancer A549 cell growth and epithelial–mesenchymal transition process by upregulation of microrna-1284. Oncology Research, 27(1), 1-8. https://doi.org/10.3727/096504018X15172738893959
Vancouver Style
Liu W, Liu N. Propofol inhibits lung cancer A549 cell growth and epithelial–mesenchymal transition process by upregulation of microrna-1284. Oncol Res. 2019;27(1):1-8 https://doi.org/10.3727/096504018X15172738893959
IEEE Style
W. Liu and N. Liu, "Propofol Inhibits Lung Cancer A549 Cell Growth and Epithelial–Mesenchymal Transition Process by Upregulation of MicroRNA-1284," Oncol. Res., vol. 27, no. 1, pp. 1-8. 2019. https://doi.org/10.3727/096504018X15172738893959



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 638

    View

  • 481

    Download

  • 0

    Like

Share Link