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miR-185 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer by Targeting KLF7

Lili Zhao*, Yao Zhang*, Jiaoxia Liu*, Wei Yin, Dan Jin, Dandan Wang*, Wei Zhang*

* Affiliated Hongqi Hospital of Mudanjiang Medical University, Mudanjiang City, P.R. China
† Mudanjiang Forestry Hospital, Mudanjiang City, P.R. China
‡ Mudanjiang Women and Children’s hospital, Mudanjiang City, P.R. China

Oncology Research 2019, 27(9), 1015-1023. https://doi.org/10.3727/096504018X15247341491655

Abstract

MicroRNAs (miRNAs) are short endogenous noncoding RNAs that frequently play vital roles in many cancer types. Herein we demonstrated that miR-185 was remarkably downregulated in NSCLC tissues compared with adjacent normal tissues. A lower level of miR-185 was associated with lymph node metastasis. Functional assays showed that upregulation of miR-185 inhibited the proliferation, colony formation, and invasion capacities of NSCLC cells in vitro. Furthermore, we found that miR-185 suppressed the epithelial–mesenchymal transition (EMT) process. Bioinformatics analysis and luciferase reporter gene assays revealed that Kruppellike factor 7 (KLF7) was the target of miR-185. Overexpression of miR-185 reduced the expression of KLF7 in NSCLC cells. Upregulation of KLF7 partly neutralized the inhibitory effects of miR-185 on the proliferation and invasion of NSCLC. Additionally, we confirmed that miR-185 suppressed the tumor growth of NSCLC A549 cells in vivo. Taken together, these results demonstrate that miR-185 acts as a suppressor by targeting KLF7 in NSCLC.

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APA Style
Zhao, L., Zhang, Y., Liu, J., Yin, W., Jin, D. et al. (2019). Mir-185 inhibits the proliferation and invasion of non-small cell lung cancer by targeting KLF7. Oncology Research, 27(9), 1015-1023. https://doi.org/10.3727/096504018X15247341491655
Vancouver Style
Zhao L, Zhang Y, Liu J, Yin W, Jin D, Wang D, et al. Mir-185 inhibits the proliferation and invasion of non-small cell lung cancer by targeting KLF7. Oncol Res. 2019;27(9):1015-1023 https://doi.org/10.3727/096504018X15247341491655
IEEE Style
L. Zhao et al., "miR-185 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer by Targeting KLF7," Oncol. Res., vol. 27, no. 9, pp. 1015-1023. 2019. https://doi.org/10.3727/096504018X15247341491655



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