Open Access

ARTICLE

Is miR-10a a tumor suppressor that modulates proliferation and invasion in high-grade bladder cancer?

THAINá RODRIGUES^1,2, PATRíCIA CANDIDO^1,3, FERES CAMARGO MALUF¹, POLIANA ROMãO¹, CAROLINA MIE MIOSHI¹, VANESSA RIBEIRO GUIMARãES¹, JULIANA ALVES DE CAMARGO¹, KARINA SERAFIM DA SILVA^1,4, GABRIEL ARANTES DOS SANTOS¹, IRAN AMORIM SILVA¹, KATIA RAMOS MOREIRA LEITE¹, WILLIAM C. NAHAS⁵, SABRINA T. REIS^1,3, RUAN PIMENTA^1,6, NAYARA IZABEL VIANA^1,7,*

1 Laboratory of Medical Investigation (LIM55), Urology Department, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, 01246-903, Brazil
2 Department of Biology, Instituto Federal de Educação, Ciência e Tecnologia de São Paulo (IFSP), Campus São Paulo, São Paulo, 01109-010, Brazil
3 Moriah Institute of Science and Education (MISE), Hospital Moriah, São Paulo, 04084-002, Brazil
4 Department of Biomedical Science, Centro Universitário São Camilo, São Paulo, 04263-200, Brazil
5Uro-Oncology Group, Urology Department, University of São Paulo Medical School and Institute of Cancer Estate of São Paulo (ICESP), São Paulo, 01246-000, Brazil
6 Urology Department, D’Or Institute for Research and Education (ID’Or), São Paulo, 01401-002, Brazil
7 Department of Bioscience, Universidade do Estado de Minas Gerais-UEMG, Passos, 37900-106, Brazil

* Corresponding Author: NAYARA IZABEL VIANA. Email:

Oncology Research 2025, 33(6), 1377-1382. https://doi.org/10.32604/or.2025.055306

Received 23 June 2024; Accepted 14 March 2025; Issue published 29 May 2025

Abstract

Objectives: Bladder Cancer (BC) is one of the most commonly diagnosed malignancies worldwide, with high rates of mortality and morbidity. It can be classified as non-muscle invasive bladder cancer (NMIBC) or muscle-invasive bladder cancer (MIBC), with radical cystectomy being the treatment for MIBC, which significantly reduces quality of life. MicroRNAs (miRs) act as critical genetic regulators, with both oncogenic and tumor-suppressive roles. MiR-10a is described as a tumor suppressor in various neoplasms, but its role in BC is controversial. This study aims to assess the activity of miR-10a in cellular invasion and proliferation in two distinct BC cell lines. Methods: The study used high-grade T24 and low-grade RT4 bladder cell lines. Cells were transfected with miR-10a mimic or a non-targeting control. Transfection efficiency was validated by qPCR. Cell proliferation was cultured for 10–14 days. Cell migration and invasion were evaluated using Matrigel. All assays were conducted in triplicate. Results: The T24 cells transfected with miR-10a presented decreased cellular proliferation and invasion compared to the Scramble (p = 0.0481 and p < 0.0001, respectively). In the RT4 cell line, there was only a significant reduction in cellular proliferation after miR-10a transfection (p = 0.0029). Conclusions: Our findings suggest that miR-10a has a tumoral suppressor role in BC, demonstrating higher efficacy in high-grade cells.

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