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Non-Polar Chemical Constituents of Atemoya and Evaluation of the Cytotoxic and Antimicrobial Activity

Suzana Vieira Rabêlo1, Fernanda Granja da Silva Oliveira2, Michele Miriam Calixto de Lira3, Livia Macedo Dutra3, Adilson Sartoratto4, Marta Cristina Teixeira Duarte4, Maria Claudia dos Santos Luciano5, Maria Francilene Souza Silva5, Cláudia do Ó Pessoa5, Manoel Odorico de Moraes Filho5, Emmanoel Vilaça Costa6, Jackson Roberto Guedes da Silva Almeida1,3,*
1 Post-Graduate Program in Biotechnology (RENORBIO), Federal Rural University of Pernambuco, Recife, Pernambuco, 52171-900, Brazil
2 Post-Graduate Program in Biotechnology, State University of Feira de Santana, Feira de Santana, Bahia, 44036-900, Brazil
3 Center for Studies and Research of Medicinal Plants (NEPLAME), Federal University of San Francisco Valley, Petrolina, Pernambuco, 56304-205, Brazil
4 Multidisciplinary Center of Chemical, Biological and Agricultural Research (CPQBA), Campinas State University, Campinas, São Paulo, 13083-970, Brazil
5 National Laboratory of Experimental Oncology (LabNOE), Federal University of Ceará, Fortaleza, Ceará, 60020-181, Brazil
6 Departament of Chemistry, Federal University of Amazonas, Manaus, Amazonas, 69067-005, Brazil
* Corresponding Author: Jackson Roberto Guedes da Silva Almeida. Email: jackson.guedes@univasf.edu.br

Phyton-International Journal of Experimental Botany https://doi.org/10.32604/phyton.2021.013305

Received 01 August 2020; Accepted 01 December 2020;


This study aimed to identify the chemical composition of essential oil from fruits (EOAF) and the hexanic crude extract from aerial parts (At-Hex) of atemoya (Annona cherimola x Annona squamosa), a hybrid belonging to the Annonaceae family. Cytotoxic and antimicrobial activity was also evaluated. OEAF was obtained by hydrodistillation using a Clevenger apparatus, and their composition was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses. Cytotoxicity was tested against human tumor cell lines HCT-116 (colon carcinoma), SF-295 (glioblastoma), OVCAR-8 (ovarian carcinoma) and HL60 (leukemia) using 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT) assay, while antimicrobial activity was conducted by bioauthography method against eleven microorganisms strains. Twenty-four compounds were identified in the EOAF and twenty-nine in At-Hex. The monoterpenes linalool (25.70%), α-pinene (10.38%), β-pinene (9.12%), transocimene (7.43%), and the sesquiterpene bicyclogermacrene (12.58%) were the major constituents of EOAF, whereas the sesquiterpene spathulenol (13.91%) was the main compound of At-Hex. At-Hex showed a high cytotoxicity against SF-295 (glioblastoma). These findings show an important chemotaxonomic contribution for the Annonaceae family, mainly for the Annona genus. Atemoya proved to be a promising source of substances with potential cytotoxic activity.


Annona; GC-MS; volatile compounds; terpenoids; biologic activities
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