Open Access
ARTICLE
Comparative Transcriptome Analysis of Salt-Stress-Responsive Genes in Rice Roots
Rui Song1, Yan Huang2, Xin Ji3, Yunfei Wei3, Qiuyuan Liu3, Shumei Li3, Juan Liu3,*, Pengfei Dong1,*
1 College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China
2 Henan Production Technology Promotion Center of Chinese Herbal Medicine, Zhenzhou, 450000, China
3 Xinyang Agriculture and Forestry University, Xinyang, 464000, China
* Corresponding Authors: Juan Liu. Email: ; Pengfei Dong. Email:
(This article belongs to the Special Issue: Integrating Agronomy and Plant Physiology for Improving Crop Production)
Phyton-International Journal of Experimental Botany 2023, 92(1), 237-250. https://doi.org/10.32604/phyton.2022.023081
Received 12 April 2022; Accepted 20 May 2022; Issue published 06 September 2022
Abstract
Soil salinity greatly impairs plant growth and crop productivity. Rice (Oryza sativa L.) is a salt-sensitive crop. To
better understand the molecular mechanisms of salt tolerance in roots, the BGISEQ-500 sequencing platform was
employed to elucidate transcriptome changes in rice roots after 0, 3, 24, and 72 h of salt stress. The results showed
that root K+ content decreased and Na+ content increased rapidly after the initial stage of salt stress, but that fresh
and dry weight in root did not significantly reduce. Compared to the control (no salt stress), 1,292, 453, and
486 differentially expressed genes (DEGs) were upregulated, respectively, and 939, 894, and 646 DEGs were
downregulated, respectively, after 3, 24, and 72 h of salt treatment. The number of DEGs was higher during
the early stage of salt stress (3 h) than in later stages (24 and 72 h). A number of DEGs involved in the response
and adaptation to salt stress were related to protein kinase and calcium-binding, plant hormone signaling and
metabolism, transcriptional regulation, metabolic pathways, antioxidant activity, and ion transport. Many of these
DEGs were activated during the early stage of salt stress (3 h). The present study reports candidate salt-stressresponsive genes with the potential to genetically improve salt tolerance in rice elsewhere.
Keywords
Supplementary Material
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Cite This Article
Song, R., Huang, Y., Ji, X., Wei, Y., Liu, Q. et al. (2023). Comparative Transcriptome Analysis of Salt-Stress-Responsive Genes in Rice Roots.
Phyton-International Journal of Experimental Botany, 92(1), 237–250. https://doi.org/10.32604/phyton.2022.023081