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ARTICLE
Effects of Heterologously Overexpressing PIP5K-Family Genes in Arabidopsis on Inflorescence Development
Mingda Yin1,#, Rui Luo1,#, Tana Liang1, Qi Wen1, Xiaotian Liang1, Yanpeng Wen1, Xuemei Hu1, Zhiyan Wang1, Chang Gao1, Wenjing Ren1, Fenglan Huang1,2,3,4,5,*
1 College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, China
2 Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission, Inner Mongolia Minzu University, Tongliao, 028000, China
3 Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Inner Mongolia Minzu University, Tongliao, 028043, China
4 Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization, Inner Mongolia Minzu University, Tongliao, 028000, China
5 Inner Mongolia Engineering Research Center of Industrial Technology Innovation of Castor, Inner Mongolia Minzu University, Tongliao, 028000, China
* Corresponding Author: Fenglan Huang. Email:
(This article belongs to the Special Issue: Advances in Molecular Genetics and Physiology towards a Better Understanding of Agricultural Crop Plants)
Phyton-International Journal of Experimental Botany 2024, 93(1), 97-117. https://doi.org/10.32604/phyton.2023.031228
Received 23 May 2023; Accepted 21 November 2023; Issue published 26 January 2024
Abstract
Castor is one of the top 10 oil crops in the world and has extremely valuable uses. Castor inflorescences directly affect yield, so the study of inflorescence development is very important in increasing castor yield. Our previous studies have shown that the
PIP5K gene family (
PIP5Ks) is associated with inflorescence development. In this study, to determine the function of each
PIP5K gene in castor, a female Lm-type castor line, aLmAB2, was used to determine the relative expression levels of the
PIP5Ks in castor inflorescences. Six
PIP5K genes were heterologously overexpressed in
Arabidopsis thaliana, the relative expression of each gene and the effect on plants was determined in
A. thaliana, and the relationships among the
PIP5Ks in castor were inferred. The expression levels of the
PIP5Ks in the female Lm-type castor line aLmAB2 were analyzed. The relative expression levels of the
PIP5K9 and
PIP5K11 genes were high (
p < 0.05) in isofemale inflorescences, and those of
PIP5K1, PIP5K2, PIP5K6, and
PIP5K8 were high (
p < 0.05) in female inflorescences but low (
p < 0.05) in bisexual inflorescences. The
PIP5Ks were heterologously overexpressed in
A. thaliana, and T3-generation plants with stable genetic resistance, i.e., AT-
PIP5K+ plants (AT-
PIP5K1+, AT-
PIP5K2+, AT-
PIP5K6+, AT-
PIP5K8+, AT-
PIP5K9+, and AT-
PIP5K11+ plants), were obtained. Biological tests of the AT-
PIP5K+ plants showed that the growth of the main stem was significantly delayed in AT-
PIP5K+ plants compared with Columbia wild-type (WT)
A. thaliana plants; the
PIP5K1 and
PIP5K2 genes promoted lateral stem growth and flower and silique development; and the
PIP5K6,
PIP5K8,
PIP5K9 and
PIP5K11 genes inhibited lateral stem growth and flower and silique development. The correlations among PIP5Ks in castor suggest that there may be a synergistic relationship among
PIP5K1,
PIP5K2, and
PIP5K6 in castor inflorescences, and
PIP5K8,
PIP5K9, and
PIP5K11 are complementary to the other three genes.
Keywords
Cite This Article
APA Style
Yin, M., Luo, R., Liang, T., Wen, Q., Liang, X. et al. (2024). Effects of heterologously overexpressing <i>pip5k</i>-family genes in arabidopsis on inflorescence development. Phyton-International Journal of Experimental Botany, 93(1), 97-117. https://doi.org/10.32604/phyton.2023.031228
Vancouver Style
Yin M, Luo R, Liang T, Wen Q, Liang X, Wen Y, et al. Effects of heterologously overexpressing <i>pip5k</i>-family genes in arabidopsis on inflorescence development. PhytonInternational J Exp Botany . 2024;93(1):97-117 https://doi.org/10.32604/phyton.2023.031228
IEEE Style
M. Yin et al., "Effects of Heterologously Overexpressing <i>PIP5K</i>-Family Genes in Arabidopsis on Inflorescence Development," PhytonInternational J. Exp. Botany , vol. 93, no. 1, pp. 97-117. 2024. https://doi.org/10.32604/phyton.2023.031228