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Antimicrobial evaluation of extracts obtained from tropical micromycetes against phytopathogens

Moreno Pérez P1,3, M Gamboa Angulo1, G Heredia2, B Canto Canché1, CM Rodríguez García1, IL Medina Baizabal1, L Peraza Echeverría1
1 Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130, Col. Chuburná de Hidalgo, Mérida 97200, Yucatán, México.
2 Red de Biodiversidad y Sistemática, Instituto de Ecología A.C., Km 2.5 antigua carretera a Coatepec No. 351, Xalapa 91070, Veracruz, México.
3 Laboratorio de microbiología médica y ambiental. Universidad Autónoma del Estado de México. Instituto Literario # 100. Col. Centro C.P. 50000. Toluca, Estado de México.
Address correspondence to: Marcela Gamboa-Angulo, e-mail:

Phyton-International Journal of Experimental Botany 2016, 85(all), 7-14. https://doi.org/10.32604/phyton.2016.85.007

Abstract

Nowadays, it is highly necessary to find more and safer agrochemicals. In this sense, micromycetes are an important source of natural products which could be used to control plant diseases. Therefore with the aims to contribute in this searching of natural products with antimicrobial applications, a total of 49 fungal strains were isolated from the Yucatan Peninsula. These fungi were cultured in fermented rice, their respective organic extracts macerated in ethyl acetate (EAE) and methanol (ME) were obtained and tested against five fungal pathogens of agricultural importance. These included Alternaria chrysanthemi, Colletotrichum gloeosporioides, Mycosphaerella fijiensis, and the bacteria Erwinia carotovora and Xanthomonas campestris using microdilution assays. The 69% of fungal extracts showed antifungal or antibacterial (2000 and 200 μg/mL, respectively) against at least one of the tested target. Strains Penicillium sp. OSE-61, Fusarium sp. OH2-30, Hypocrea lixii OSN-37 and Rhizoctonia solani OSE-73 showed activity against at least three of the five study pathogens. The most active EAE were partitioned, and its low (A), medium (B) polarity and precipitated (C) were obtained and assessed. The fraction A from Fusarium KS-15 displayed the lowest Minimum Inhibitory Concentration (MIC ≤25 μg/mL) and bactericide effect on X. campestris; fraction B of Penicillium sp. OSE-61 was relatively greater than the previous on A. chrysanthemi (MIC ≤500 μg/mL). Last fraction also exhibited good inhibitory effect on C. gloeosporioides. Finally, fraction B of H. lixii OSN-37 displayed promissory antifungal effect on C. gloeosporioides y M. fijiensis (MIC = 1000 μg/mL). This research contributes to enrich the limited knowledge on the biological activity of native anamorphic fungi of the sinkholes of Yucatan and its potential use in biotechnological applications in agriculture.

Keywords

Bioassays, Fungal extracts, Fusarium, Hypocrea, Microfungi, Penicillium, Sinkholes.

Cite This Article

P, M. P., Angulo, M. G., Heredia, G., Canché, B. C., García, C. R. et al. (2016). Antimicrobial evaluation of extracts obtained from tropical micromycetes against phytopathogens. Phyton-International Journal of Experimental Botany, 85(all), 7–14.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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