Table of Content

Open Access iconOpen Access

ARTICLE

crossmark

Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to Sclerotinia sclerotiorum in Brassica napus

Jingsen Liu1,2, Jinqi Ma1,2, Ai Lin1,2, Chao Zhang1,2, Bo Yang1,2, Liyuan Zhang1,2, Lin Huang1,2, Jiana Li1,2,*

1 Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Chongqing, 400715, China
2 Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China

* Corresponding Author: Jiana Li. Email: email

Phyton-International Journal of Experimental Botany 2020, 89(2), 253-273. https://doi.org/10.32604/phyton.2020.09334

Abstract

Invertase (INV), a key enzyme in sucrose metabolism, irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose, thus playing important roles in plant growth, development, and biotic and abiotic stress responses. In this study, we identified 27 members of the BnaINV family in Brassica napus. We constructed a phylogenetic tree of the family and predicted the gene structures, conserved motifs, cis-acting elements in promoters, physicochemical properties of encoded proteins, and chromosomal distribution of the BnaINVs. We also analyzed the expression of the BnaINVs in different tissues and developmental stages in the B. napus cultivar Zhongshuang 11 using qRT-PCR. In addition, we analyzed RNA-sequencing data to explore the expression patterns of the BnaINVs in four cultivars with different harvest indices and in plants inoculated with the pathogenic fungus Sclerotinia sclerotiorum. We used WGCNA (weighted coexpression network analysis) to uncover BnaINVregulatory networks. Finally, we explored the expression patterns of several BnaINV genes in cultivars with long (Zhongshuang 4) and short (Ningyou 12) siliques. Our results suggest that BnaINVs play important roles in the growth and development of rapeseed siliques and the defense response against pathogens. Our findings could facilitate the breeding of high-yielding B. napus cultivars with strong disease resistance.

Keywords


Cite This Article

APA Style
Liu, J., Ma, J., Lin, A., Zhang, C., Yang, B. et al. (2020). Genome-wide identification and expression profiling suggest that invertase genes function in silique development and the response to <i>sclerotinia sclerotiorum</i> in <i>brassica napus</i>. Phyton-International Journal of Experimental Botany, 89(2), 253-273. https://doi.org/10.32604/phyton.2020.09334
Vancouver Style
Liu J, Ma J, Lin A, Zhang C, Yang B, Zhang L, et al. Genome-wide identification and expression profiling suggest that invertase genes function in silique development and the response to <i>sclerotinia sclerotiorum</i> in <i>brassica napus</i>. Phyton-Int J Exp Bot. 2020;89(2):253-273 https://doi.org/10.32604/phyton.2020.09334
IEEE Style
J. Liu et al., "Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to <i>Sclerotinia sclerotiorum</i> in <i>Brassica napus</i>," Phyton-Int. J. Exp. Bot., vol. 89, no. 2, pp. 253-273. 2020. https://doi.org/10.32604/phyton.2020.09334



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 2735

    View

  • 1763

    Download

  • 0

    Like

Share Link