Open Access
ARTICLE
Identification and Genetic Analysis of a Novel Allelic Variation of Brittle-1 with Endosperm Mutant in Maize
Sen Wang1, Fei Zheng1, Meijing Zhang1, Jun Tu2, Yanping Chen1,3, Jianhua Yuan1,3, Qingchang Meng1,*
1 Institute of Food Crops, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China
2 Jiangsu Zhongjiang Seed Co., Ltd., Nanjing, 211500, China
3 Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, 210095, China
* Corresponding Author: Qingchang Meng. Email:
Phyton-International Journal of Experimental Botany 2020, 89(4), 861-871. https://doi.org/10.32604/phyton.2020.011762
Received 28 May 2020; Accepted 18 July 2020; Issue published 09 November 2020
Abstract
Endosperm mutants are critical to the studies on both starch synthesis
and metabolism and genetic improvement of starch quality in maize. In the present study, a novel maize endosperm mutant A0178 of natural variation was used
as the experimental material and identified and then characterized. Through phenotypic identification, genetic analysis, main ingredients measurement and
embryo rescue, development of genetic mapping population from A0178, the
endosperm mutant gene was located. The results showed that the mutant exhibited
extremely low germination ability as attributed to the inhibited embryo development, and amounts of sugars were accumulated in the mutant seeds and more
sugars content was detected at 23 days after pollination (DAP) in A0178 than
B73. Employing genetic linkage analysis, the mutant trait was mapped in the
bin 5.04 on chromosome 5. Sequence analysis showed that two sites of base transversion and insertion presented in the protein coding region and non-coding
region of the mutant
brittle-1 (
bt1), the adenylate translocator encoding gene
involved in the starch synthesis. The single base insertion in the coding region
cause frameshift mutation, early termination and lose of function of
Brittle-1
(
BT1). All results suggested that
bt1 is a novel allelic gene and the causal gene
of this endosperm mutant, providing insights on the mechanism of endosperm formation in maize.
Keywords
Cite This Article
Wang, S., Zheng, F., Zhang, M., Tu, J., Chen, Y. et al. (2020). Identification and Genetic Analysis of a Novel Allelic Variation of
Brittle-1 with Endosperm Mutant in Maize.
Phyton-International Journal of Experimental Botany, 89(4), 861–871.