Open Access

ARTICLE

Selection of Stable Reference Genes for Quantitative Real-Time PCR on Paeonia ostii T. Hong et J. X. Zhang Leaves Exposed to Different Drought Stress Conditions

Yuting Luan¹, Cong Xu¹, Xiaoxiao Wang¹, Daqiu Zhao¹, Jun Tao^1,2,*

1 College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China
2 Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China

* Corresponding Author: Jun Tao. Email:

Phyton-International Journal of Experimental Botany 2022, 91(5), 1045-1059. https://doi.org/10.32604/phyton.2022.018767

Received 16 August 2021; Accepted 27 October 2021; Issue published 24 January 2022

Abstract

The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, ACT1, ACT2, GAPDH, eIF1, eIF2, α-TUB, β-TUB, TBP, RNA Pol II and RP II, were calculated for a suitable reference gene selection in Paeonia ostii T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one. In addition, the geNorm program provided the optimal choice of two reference gene combination, RNA Pol II and β-TUB, for qRT-PCR normalization in P. ostii subjected to different drought stress levels. Our research provided convenience for gene expression analysis in P. ostii under drought stress and promoted research of effective methods to alleviate P. ostii drought stress in the future.

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Keywords

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