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Identification of Resistance to Pathogenesis Related Protein GmPR1L in Tobacco Botrytis cinerea Infection

Yeyao Du1,#, Ye Zhang2,#, Yang Song1, Zhuo Zhang1, Sujie Fan1, Hanzhu Zhang1, Piwu Wang1,*

1 College of Agronomy, Jilin Agricultural University, Changchun, China
2 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China

* Corresponding Author: Piwu Wang. Email: email
# These authors contributed equally to this work

(This article belongs to the Special Issue: Symbiotic Associations for Nutrients Management and Complexes Formation for Better Agricultural Crops Productivity under Biotic and Abiotic Stresses)

Phyton-International Journal of Experimental Botany 2023, 92(6), 1907-1920. https://doi.org/10.32604/phyton.2023.027607

Abstract

Soybean (Glycine max (Linn.) Merr.) annual leguminous crop is cultivated all over the world. The occurrence of diseases has a great impact on the yield and quality of soybean. In this study, based on the RNA-seq of soybean variety M18, a complete CDS (Coding sequence) GmPR1L of the pathogenesis-related protein 1 family was obtained, which has the ability to resist fungal diseases. The overexpression vector and interference expression vector were transferred into tobacco NC89, and the resistance of transgenic tobacco (Nicotiana tabacum L.) to Botrytis cinerea infection was identified. The results show that: Compared with the control, the activities of related defense enzymes SOD (Superoxide dismutase), POD (Peroxidase), PAL (L-phenylalanine ammonia-lyase) and PPO (Polyphenol oxidase) in the over-expressed transgenic tobacco OEA1 and OEA2 increased to different degrees, and increased significantly at different infection time points. The activities of defense enzymes in the interfering strains IEA1 and IEA2 were significantly lower than those in the control strains. The results of resistance level identification showed that the disease spot rate of OEA1 was significantly lower than that of the control line, and the disease spot rate of OEA2 was significantly lower than that of the control line. The plaque rate of the interfering expression line IEA1-IEA2 was significantly higher than that of the control line. It is preliminarily believed that the process related protein GmPR1L can improve the resistance of tobacco to B. cinerea.

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APA Style
Du, Y., Zhang, Y., Song, Y., Zhang, Z., Fan, S. et al. (2023). Identification of resistance to pathogenesis related protein <i>gmpr1l</i> in tobacco <i>botrytis cinerea</i> infection. Phyton-International Journal of Experimental Botany, 92(6), 1907-1920. https://doi.org/10.32604/phyton.2023.027607
Vancouver Style
Du Y, Zhang Y, Song Y, Zhang Z, Fan S, Zhang H, et al. Identification of resistance to pathogenesis related protein <i>gmpr1l</i> in tobacco <i>botrytis cinerea</i> infection. Phyton-Int J Exp Bot. 2023;92(6):1907-1920 https://doi.org/10.32604/phyton.2023.027607
IEEE Style
Y. Du et al., "Identification of Resistance to Pathogenesis Related Protein <i>GmPR1L</i> in Tobacco <i>Botrytis cinerea</i> Infection," Phyton-Int. J. Exp. Bot., vol. 92, no. 6, pp. 1907-1920. 2023. https://doi.org/10.32604/phyton.2023.027607



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