Open Access
ARTICLE
Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds
Cong Hou1, Kang Ning1, Xiuye Wei1, Yufei Cheng1, Huatao Yu1, Haibin Yu2, Xia Liu1,*, Linlin Dong1,*
1 School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China
2 Yunnan Hemp Industrial Investment Co., Ltd., Kunming, 650000, China
* Corresponding Authors: Xia Liu. Email: ; Linlin Dong. Email:
(This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
Phyton-International Journal of Experimental Botany 2023, 92(7), 2087-2103. https://doi.org/10.32604/phyton.2023.029041
Received 28 January 2023; Accepted 28 March 2023; Issue published 29 May 2023
Abstract
Abscisic acid (ABA) is involved in regulating diverse biological processes, but its signal transduction genes and roles in hemp seed germination are not well known. Here, the ABA signaling pathway members,
PYL,
PP2C and
SnRK2 gene families, were identified from the hemp reference genome, including 7
CsPYL (pyrab-actin resistance1-like, ABA receptor), 8
CsPP2CA (group A protein phosphatase 2c), and 7
CsSnRK2 (sucrose nonfermenting1-related protein kinase 2). The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage. Exogenous ABA (1 or 10 μM) treatment had a significant regulatory effect on the selected
PYL,
PP2C,
SnRK2 gene families.
CsAHG3 and
CsHAI1 were most significantly affected by exogenous ABA treatment. Yeast two-hybrid experiments were performed to reveal that CsPYL5, CsSnRK2.2, and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was ABA-independent. Our results indicated that
CsPYL5,
CsSnRK2.2,
CsSnRK2.3 and
CsPP2CA7 might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination stages. This study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination.
Keywords
Cite This Article
Hou, C., Ning, K., Wei, X., Cheng, Y., Yu, H. et al. (2023). Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds.
Phyton-International Journal of Experimental Botany, 92(7), 2087–2103. https://doi.org/10.32604/phyton.2023.029041