Open Access

ARTICLE

Transcriptome and Metabolome Revealed the Mechanism of NtBRL3 Overexpression Tobacco (Nicotiana tabacum L. K326) in Response to Drought Stress

Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu^*

College of Tobacco Science of Guizhou University, Guizhou Key Laboratory for Tobacco Quality, Guiyang, 550025, China

* Corresponding Author: Yang Liu. Email:

(This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)

Phyton-International Journal of Experimental Botany 2023, 92(9), 2555-2576. https://doi.org/10.32604/phyton.2023.030301

Received 30 March 2023; Accepted 19 May 2023; Issue published 28 July 2023

Abstract

Drought has severely affected the yield and quality of commercial crops. The BRI1 family plays an important role in plant response to drought stress, and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana. In this study, NtBRL3 was constructed as a vector and genetically transformed to obtain ‘N. Tobacco K326’ overexpression of NtBRL3. The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed. The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions, and 85 significantly differentially metabolites and 106 significantly differentially expressed genes were identified in the metabolome and transcriptome analyses, respectively. Transgenic tobacco NtBRL3ox demonstrated an excessive accumulation of drought-related metabolites, sugars such as sucrose and maltotetraose, and amino acids such as proline, compared with WT. We discovered drought-related differential genes in the root transcriptome, among which LOX6, RD22, WSD1, CCD8, and UGT were key genes which play an important role in plant response to drought stress. Our results demonstrate that NtBRL3 overexpression in K326 enhances drought resistance in transgenic tobacco.

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Keywords

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Supplementary Material

Supplementary Material File

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