Open Access

REVIEW

Physiological and Molecular Mechanisms of Freezing in Plants

Ali Salehi Sardoei^1,*, Bahman Fazeli-Nasab²

1 Crop and Horticultural Science Research Department, South Kerman Agricultural and Natural Resources Research and Education Center, AREEO, Jiroft, 78617-46411, Iran
2 Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, 98613-35856, Iran

* Corresponding Author: Ali Salehi Sardoei. Email:

(This article belongs to the Special Issue: Metabolic Mechanisms of Plant Responses to Stress)

Phyton-International Journal of Experimental Botany 2025, 94(6), 1601-1630. https://doi.org/10.32604/phyton.2025.064729

Received 22 February 2025; Accepted 26 May 2025; Issue published 27 June 2025

Abstract

The ability of plants to tolerate cold is a complex process. When temperatures drop or freeze, plant tissues can develop ice, which dehydrates the cells. However, plants can protect themselves by preventing ice formation. This intricate response to cold stress is regulated by hormones, photoperiod, light, and various factors, in addition to genetic influences. In autumn, plants undergo morphological, physiological, biochemical, and molecular changes to prepare for the low temperatures of winter. Understanding cellular stress responses is crucial for genetic manipulation aimed at enhancing cold resistance. Early autumn frosts or late spring chills can cause significant damage to plants, making it essential to adapt in autumn to survive winter conditions. While the general process of acclimatization is similar across many plant species, variations exist depending on the specific type of plant and regional conditions. Different plant organs exhibit varying degrees of damage from cold stress, and by applying agricultural principles, potential damage can be largely controlled. Timely reinforcement and stress prevention can minimize cold-related damage. Research has shown that in temperate climates, low temperatures restrict plant growth and yield. However, the intricate structural systems involved remain poorly understood. Over the past decade, studies have focused on the molecular mechanisms that enable plants to adapt to and resist cold stress. The gene signaling system is believed to play a crucial role in cold adaptation, and researchers have prioritized this area in their investigations. This study critically examines plant responses to cold stress through physiological adaptations, including calcium signaling dynamics, membrane lipid modifications, and adjustments in antioxidant systems. These mechanisms activate downstream gene expression and molecular functions, leading to key resistance strategies. Additionally, we explore the regulatory roles of endogenous phytohormones and secondary metabolites in cold stress responses. This review aims to enhance our foundational understanding of the mechanisms behind plant cold adaptation.

---

Keywords

---