Open Access

ARTICLE

A Comprehensive Expression Atlas of Nicotiana tabacum: Revealing Specificity Expression Patterns and Regulatory Variants

Shizhou Yu^1,2, Jie Zhang¹, Linggai Cao¹, Jie Liu¹, Peng Lu³, Jiemeng Tao³, Xueliang Ren¹, Zhixiao Yang^1,2,*

1 Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang, China
2 Guizhou Provincial Key Laboratory for Tobacco Quality Improvement and Efficiency Enhancement, Guiyang, China
3 China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China

* Corresponding Author: Zhixiao Yang. Email:

Phyton-International Journal of Experimental Botany 2026, 95(2), 6 https://doi.org/10.32604/phyton.2026.073509

Received 19 September 2025; Accepted 08 January 2026; Issue published 28 February 2026

Abstract

Tobacco (Nicotiana tabacum, 2n = 48) is a key non-food economic crop, yet its stress response and gene regulatory mechanisms remain poorly understood. By analyzing 603 transcriptome datasets, this study identified 1405 tissue-specific genes, revealing tissue-specific synthesis of terpenoids and other ecologically important secondary metabolites in sepals and other tissues. Comparative stress-response analysis highlighted distinct gene expression patterns in leaves and roots under biotic and abiotic stresses. Additionally, 28,396 expression quantitative trait loci (eQTLs) were mapped in leaves, offering valuable genetic regulatory markers. These findings provide crucial insights into tobacco’s gene expression characteristics and their functional implications, serving as a foundation for future research.

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Keywords

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Supplementary Material

Supplementary Material File

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