Mengjie Xu, Anbin Wang, Tonghong Zuo, Hecui Zhang, Zhihao Hu, Liquan Zhu^*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 157-179, 2025, DOI:10.32604/phyton.2025.060566 - 24 January 2025

Abstract Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the characterization and expression profiling of CMLs genes in tobacco is retrievable. In this study, a comprehensive whole-genome identification and analysis, and 75 NtCML genes were identified in tobacco, each containing two to four EF-hand domains. Most NtCML proteins exhibited conserved gene structures and motifs. Notably, most NtCML proteins were intron-less and distributed across 18 chromosomes. Two pairs of tandemly duplicated genes and seven pairs of segmentally More >

Qiang Zhang^1,#, Dongsheng Gai^1,#, Yong Liu¹, Weiyang Liu¹, Pengcheng Fu¹, Xiwen Shao¹, Xuanhe Liang², Yanqiu Geng^1,*, Liying Guo^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 269-279, 2025, DOI:10.32604/phyton.2025.060551 - 24 January 2025

Abstract The yield of direct-seeded rice (DSR) was constrained by inadequate grain filling. Recent studies have indicated that paclobutrazol application plays a significant role in enhancing crop agronomic traits and increasing yield. This study aimed to examine the effects of paclobutrazol seed soaking (PSS) on non-structural carbohydrate accumulation and grain enrichment in DSR, potentially providing a theoretical foundation for achieving high-yield DSR cultivation. The experiment utilized two rice varieties, Jiyujing (JYJ) and Jijing305 (JJ305), with seeds soaked in paclobutrazol concentrations of 0 mg L⁻¹ and 100 mg L⁻¹. PSS demonstrated increased chlorophyll content, net photosynthetic rate, and… More >

Samiah Hamad Al-Mijalli¹, Hanae Naceiri Mrabti², Amine Elbouzidi^3,*, Naglaa S. Ashmawy^4,5, Amine Batbat⁶, Emad M. Abdallah⁷, Wafa Laaboudi⁸, Mohammed Aladhadh⁹, Fahad M. Alshabrmi¹⁰, Sulaiman Mohammed Alnasser¹¹, Mohamed Addi³, Naoufal El Hachlafi¹²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 209-227, 2025, DOI:10.32604/phyton.2025.060438 - 24 January 2025

Abstract Thymus serpyllum L., often known as wild thyme, has been used since ancient times due to its multifaceted culinary and medicinal attributes. It is usually utilized in folk medicine to manage different health issues. This work aimed to investigate the chemical composition and biological characteristics of T. serpyllum essential oil (EO), including its antimicrobial, antioxidant, and anti-inflammatory capabilities. Moreover, we have prompted an in-silico simulation to reveal the underlying mode of action of these properties. The chemical characterization of T. serpyllum (EO) by Gas Chromatography-Mass Spectrometry (GC-MS) indicated sabinene (17.33%), terpinen-4-ol (11.73%), phellandral (13.18%), and thymol (10.54%) as main… More >

Hocine Bougrine^1,2, Salah Hadjout^1,*, Mohamed Zouidi¹, Abdeldjalil Belkendil¹, Amer Zeghmar¹, Chaouki Boulekdam¹, Walid Ouaret³, Walid Soufan⁴, Fathi Abdellatif Belhouadjeb⁵, Amar Mebarkia²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 251-267, 2025, DOI:10.32604/phyton.2025.060096 - 24 January 2025

Abstract Salinity stress is a major challenge for global agriculture, particularly in arid and semi-arid regions, limiting plant productivity due to water and soil salinity. These conditions particularly affect countries along the southern Mediterranean rim, including Algeria, which primarily focuses on pastoral and forage practices. This study investigates salinity tolerance and ecotypic variability in Vicia narbonensis L., a fodder legume species recognized for its potential to reclaim marginal soils. Morphological, physiological, and biochemical responses were assessed in three ecotypes (eco2, eco9, and eco10) exposed to different salinity levels (low, moderate, and severe). The study was conducted using… More >

Pamela Lippi, Giovan Battista Mattii, Eleonora Cataldo^*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 33-64, 2025, DOI:10.32604/phyton.2025.059997 - 24 January 2025

Abstract Biochar has emerged as a promising tool for enhancing vineyard sustainability by improving soil properties and mitigating climate change impacts. This review highlights key findings on biochar’s role in viticulture, focusing on its effects on soil fertility, water retention, and plant physiology. Field and pot studies demonstrate that biochar amendments enhance soil structure, increase cation exchange capacity (CEC), and promote water availability, leading to improved drought resistance in grapevines. However, the impacts on grape yield, physiology, and quality remain inconclusive, with some studies reporting benefits while others show neutral effects. Future research should focus on More >

Su-Ee Lau^1,2,*, Lucas Wei Tze Lim³, Mohd Fadhli Hamdan⁴, Colin Chan⁵, Noor Baity Saidi⁶, Janna Ong-Abdullah⁶, Boon Chin Tan^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 1-31, 2025, DOI:10.32604/phyton.2025.059930 - 24 January 2025

Abstract Abiotic stresses such as drought, heat, salinity, and heavy metal contamination severely affect global agricultural productivity. Between 2005 and 2015, droughts caused losses of approximately USD 29 billion in developing countries, and from 2008 to 2018, droughts accounted for over 34% of crop and livestock yield losses, totaling about USD 37 billion. To support the growing human population, agricultural output must increase substantially, necessitating a 60%–100% rise in crop productivity to meet the escalating demand. To address environmental challenges, organic, inorganic, and microbial biostimulants are increasingly employed to enhance plant resilience through various morphological, physiological,… More >

Yanlin Pan¹, Yibin Zhang¹, Xingui Wang¹, Hongbo Qin¹, Lunfa Guo^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 123-136, 2025, DOI:10.32604/phyton.2025.059598 - 24 January 2025

Abstract Derris fordii and Derris elliptica belong to the Derris genus of the Fabaceae family, distinguished by their high isoflavonoid content, particularly rotenoids, which hold significance in pharmaceuticals and agriculture. Rotenone, as a prominent rotenoid, has a longstanding history of use in pesticides, veterinary applications, medicine, and medical research. The accumulation of rotenoids within Derris plants adheres to species-specific and tissue-specific patterns and is also influenced by environmental factors. Current research predominantly addresses extraction techniques, pharmacological applications, and pesticide formulations, whereas investigations into the biosynthesis pathway and regulatory mechanism of rotenoids remain relatively scarce. In this study, we… More >

Yan Wu¹, Xuerui An¹, Haofan Lv¹, Zhiqiang Zhao¹, Wei Liu^1,2,*, Chunpeng Wan^1,3,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 199-207, 2025, DOI:10.32604/phyton.2025.059581 - 24 January 2025

Abstract A novel pair of oleanane nor-triterpenes, with compound 1 featuring a unique 18α-H structure, was isolated from mastic, and this compound represents a noteworthy new entity not previously reported in the literature. The absolute configurations of their structures were further determined using a combination of different analytical methods such as NMR, high-resolution mass spectrometry (HR-MS), ultraviolet (UV), infrared (IR) and single-crystal X-ray diffraction (SXRD). The compound actively mitigated inflammations by efficiently quenching nitric oxide (NO) synthesis within an ex vivo system using lipopolysaccharide activated murine macrophage RAW264.7 cells. Moreover, compound 1 exhibit a better IC₅₀ concentration of More >

Fei Li^1,2, Meili Ding¹, Hui Yuan¹, Siping Wang¹, Bin Liang^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 243-250, 2025, DOI:10.32604/phyton.2025.059499 - 24 January 2025

Abstract This study investigated the effects of chlorine-based fertilizers under varying nitrogen solution concentrations in a soilless culture system. The experiment included four nitrogen solution concentration levels, with nitrogen concentrations of 6 mmol/L (C1), 12 mmol/L (C2), 18 mmol/L (C3), and 24 mmol/L (C4). Each nutrient concentration level was further divided into four chloride ion treatments (R1, R2, R3, and R4), where 100%, 60%, 33%, and 0% of the NH₄⁺ and K⁺ ions were derived from NH₄Cl and KCl, respectively. The length, surface area and volume of root were significantly higher by 25.3%~136.9%, 40.1%~173.1%, 27.9%~178.0%, respectively, in the… More >

Fayez Althobaiti¹, Hadeer Darwish^1,*, Raghad Alruqayb¹, Saqer S. Alotaibi¹, Fahad E. Alharthi², Ibrahim Jafri¹, Sarah Awwadh Altalhi¹, Ghadi Alsharif^3,4, Hussam Awwadh E Althagafi⁵, Hesham Noureldeen⁶, Sarah Alharthi⁷, Jawaher Albaqami², Najla Amin T. Al Kashgry², Ahmed Noureldeen²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 181-198, 2025, DOI:10.32604/phyton.2025.059387 - 24 January 2025

Abstract The interest in using the Datura stramonium plant is due to its natural products, which are used in many pharmaceutical industries. The objective of the current study was to assess the therapeutic and cytotoxic effects of the D. stramonium plant on two types of human cancer cell models (MCF7 and HT29) in vitro. A soxhlet apparatus was used to obtain methanolic extract from dried plant leaves. The recovered crude, after the solvent had evaporated, was then dispersed at varied concentrations of extract 100, 50, 20, and 0.0 µg/mL and tested to see how the cells responded. Also,… More >