Haoxue Wu^#, Xiaohan Zhang^§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321

Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression levels of… More >

Yanhua Li^1,#, Hualei Huang^1,#, Youming Shi¹, Shuqin Huang¹, Tao Liu¹, Changming Xiao¹, Xiaoqing Tian², Ping Zhao¹, Xiaoyan Dai³, Taocui Huang^1,*, Yan Zhou^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 815-835, 2023, DOI:10.32604/phyton.2023.023928

Abstract Gibberellin 2-oxidases (GA2ox) are important enzymes that maintain the balance of bioactive GAs in plants. GA2ox genes have been identified and characterized in many plants, but these genes were not investigated in Brassica napus. Here, we identified 31 GA2ox genes in B. napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes. Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm, and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons. Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups, including… More >

Ruizhi Huang¹, Huasheng Yu², Yong Yang¹, Heqin Liu², Xuelong Wu¹, Zhihong Liu¹, Haiyan He¹, Gengwei Wu¹, Wengjia Wang¹, Hua Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 333-348, 2023, DOI:10.32604/phyton.2022.023252

Abstract As global temperature rise, the threat of heat stress to rapeseed production is becoming more obvious. Exploring the response characteristics of two important biological pathways, oil accumulation and photosynthesis, to heat stress during B. napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed. The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B. napus germplasms with different oil content and environmental sensitivity, including 6 rapeseed varieties which exhibited environment-sensitive/insensitive and with high, medium or low oil content, were tested by whole plant heat stress or the in vitro silique culture system.… More >

Reza Shokri-Gharelo¹, Ali Bandehagh^1,*, Mohammad Anwar Hossain^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1403-1417, 2022, DOI:10.32604/phyton.2022.020431

Abstract Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola (Brassica napus L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to drought stress. The experiment used a completely randomized design, implemented in a hydroponic system under greenhouse conditions.… More >