CHAO NIU^1,#, RUOFEI SUN^1,#, XIAOGANG LI², BO LI², XIAODONG HE^1,*

BIOCELL, Vol.47, No.10, pp. 2301-2312, 2023, DOI:10.32604/biocell.2023.029935

Abstract Objective: Breast cancer is a major cancer threatening the health of women globally. To elucidate the effect of the circHIAT1/miR-19a-3p/phosphatase and tensin homolog (PTEN) axis on regulating the malignant phenotype of breast cancer cells. Methods: The mRNA expression pattern of circHIAT1, miR-19a-3p, and PTEN was checked by real-time quantitative polymerase chain reaction. Then, the knockdown assay was carried out to explore the effect of circHIAT1 and miR-19a-3p on breast cancer. The relative cell experiments, including MTT assay, scratch assay, transwell invasion assay, and flow cytometry analysis, were conducted to verify the influence of circHIAT1 and miR-19a-3p on breast cancer cells.… More >

YANG GU^1,#,*, MINGGUANG BI^2,#, DEHUI CHEN³, NING NI⁴, JIANMING CHEN^1,*

BIOCELL, Vol.47, No.9, pp. 2069-2080, 2023, DOI:10.32604/biocell.2023.029989

Abstract Background: The underlying mechanism of sciatic nerve injury (SNI) is a common motor functional disorder, necessitates further research. Methods: A rat model of SNI was established, with the injury group subjected to compressive injury of the right sciatic nerve exposed at the midpoint of the thigh and the sham surgery group undergoing the same surgical procedure. An oxygen-glucose deprivation model was employed to simulate in vitro SNI in PC12 cells. Following data acquisition and quality control, differentially expressed proteins (DEPs) in each model were identified through differential analysis, and enrichment analysis was used to explore the potential functions and pathways… More >

MUNEEBA MALIK¹, MAMOONA MAQBOOL², TOOBA NISAR³, TAZEEM AKHTER⁴, JAVED AHMED UJAN^5,6, ALANOOD S. ALGARNI⁷, FAKHRIA A. AL JOUFI⁸, SULTAN SHAFI K. ALANAZI⁹, MOHAMMAD HADI ALMOTARED¹⁰, MOUNIR M. SALEM BEKHIT¹¹, MUHAMMAD JAMIL^12,*

Oncology Research, Vol.31, No.6, pp. 899-916, 2023, DOI:10.32604/or.2023.030760

Abstract The low survival rate of Kidney renal clear cell carcinoma (KIRC) patients is largely attributed to cisplatin resistance. Rather than focusing solely on individual proteins, exploring protein-protein interactions could offer greater insight into drug resistance. To this end, a series of in silico and in vitro experiments were conducted to identify hub genes in the intricate network of cisplatin resistance-related genes in KIRC chemotherapy. The genes involved in cisplatin resistance across KIRC were retrieved from the National Center for Biotechnology Information (NCBI) database using search terms as “Kidney renal clear cell carcinoma” and “Cisplatin resistance”. The genes retrieved were analyzed… More >

Tahira Kalsoom¹, Touqeer Ahmed¹, Muhammad Azam Khan¹, Mirza Hasanuzzaman^2,*, Mukhtar Ahmed^3,*, Stefaan P. O. Werbrouck⁴

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2429-2450, 2023, DOI:10.32604/phyton.2023.030846

Abstract Potato propagation through tubers and seed is an inefficient way of propagation thus we propose an alternative method in this study which is in vitro propagation. Plantlets were raised from True Potato Seeds (TPS) in test tubes containing 10 mL of Murashige Skoog (MS) medium. Subculturing was done after every 21 days and after four subsequent subculturing, when there was an adequate quantity of stock material, uninodal cuttings of about 3–4 mm in length were prepared from in vitro proliferated potato plants by excising leaves of 1–2 mm on each side of the node. Prepared uninodal cuttings were cultured on… More >

LI TIAN^1,#, YONGYI HUANG^1,#, BAOZHEN ZHANG^2,#, YI SONG^1,#, LIN YANG³, QIANQIAN CHEN¹, ZHENG WANG³, YILING WANG¹, QIHAN HE¹, WENHAN YANG¹, SHUYONG YU⁴, TIANYU LU⁵, ZICHEN LIU¹, KAIPING GAO^1,*, XIUJUN FAN^2,*, JIAN SONG^4,*, RIHONG ZHAI^1,*

Oncology Research, Vol.31, No.4, pp. 463-479, 2023, DOI:10.32604/or.2023.028791

Abstract Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanisms of lncRNAs in ESCC are still incompletely understood and therapeutic attempts for in vivo targeting cancer-associated lncRNA remain a challenge. By RNA-sequencing analysis, we identified that LLNLR-299G3.1 was a novel ESCC-associated lncRNA. LLNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion. Silencing of LLNLR-299G3.1 with ASO (antisense oligonucleotide) resulted in opposite effects. Mechanistically, LLNLR-299G3.1 bound to cancer-associated RNA binding proteins and regulated the expression of cancer-related… More >

BOWEN PENG¹, YUN GE¹, GANG YIN^2,3,*

BIOCELL, Vol.47, No.7, pp. 1519-1535, 2023, DOI:10.32604/biocell.2023.027026

Abstract Background: Tanshinone IIA, one of the main ingredients of Danshen, is used to treat hepatocellular carcinoma (HCC). However, potential targets of the molecule in the therapy of HCC are unknown. Methods: In this study, we collected the tanshinone IIA targets from public databases for investigation. We screened differentially expressed genes (DEGs) across HCC and normal tissues using mRNA expression profiles from The Cancer Genome Atlas (TCGA). Univariate Cox regression analysis and least absolute shrinkage and selection operator (LASSO) Cox regression models were used to identify and construct the prognostic gene signature. Results: Finally, we discovered common genes across tanshinone IIA… More >

Mochamad Chalid^1,*, Gifrandy Gustiraharjo¹, Azizah Intan Pangesty¹, Alyssa Adyandra¹, Yudan Whulanza², Sugeng Supriadi²

Journal of Renewable Materials, Vol.11, No.7, pp. 3043-3056, 2023, DOI:10.32604/jrm.2023.026788

Abstract Polyethylene glycol (PEG) was added at different concentrations to the blend of poly(L-lactic acid) (PLLA) and poly(D,L-lactic acid)(PDLLA) to tailor the properties. The differential scanning calorimetry (DSC) measurement showed that all blends were miscible due to shifting a single glass transition temperature into a lower temperature for increasing PEG content. The DSC, FTIR, and XRD results implied the crystallinity enhancement for PEG content until 8 wt%, then decreased at 12 wt% PEG. The XRD result indicated the homo crystalline phase formation in all blends and no stereocomplex crystal. The in vitro degradation study indicated that PEG content is proportional to… More > Graphic Abstract

Esraa E. Shalan¹, Said S. Soliman¹, Ahmed A. Mahmoud¹, Jameel M. Al-Khayri^2,*, Salha M. ALshamrani³, Fatmah A. Safhi⁴, Areej S. Jalal⁴, Diaa Abd El-Moneim⁵, Abdallah A. Hassanin^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2183-2196, 2023, DOI:10.32604/phyton.2023.028537

Abstract Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication. In this research, nine multiplication media (MM) augmented with different concentrations of 6-benzyl adenine (BA), Kinetin (Kin), and Thidiazuron (TDZ), Three rooting media (RM) supplemented with three levels of α-naphthalene acetic acid (NAA) and three types of soil mixtures (v/v); Coco peat/Vermiculite/Sand (CVS), Peat moss/Perlite/Sand (PPS) and Peat moss/Perlite (PP) were used in the micropropagation protocol of daylily plants. MM2 showed the maximum shoot length and the number of leaves, while MM9 showed the maximum number… More >

Sugey Vásquez-Hernández, Joaquín Adolfo Montes-Molina^*, Federico Antonio Gutiérrez-Miceli, Sheila Jazmín Reyes-Zambrano, Carlos Alberto Lecona-Guzmán^*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2065-2078, 2023, DOI:10.32604/phyton.2023.028784

Abstract Biotechnological techniques provide a viable alternative to help improve and increase the production of plant species of agricultural and economic importance, which have been affected over the years by climate change, increasing their susceptibility to pests and/or diseases, generating losses in production as well as a decrease in their regenerative and genetic diversity. The application of biotechnological techniques such as in vitro mutagenesis offers a viable option for the generation of crops that are resistant to the different factors caused by abiotic and biotic stress. In vitro mutagenesis has been used in an efficient way to generate genetic changes in… More >

Ibrahim Hmmam^1,*, Ali Raza², Ivica Djalovic³, Nagwa Khedr¹, Abdou Abdellatif¹

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1695-1711, 2023, DOI:10.32604/phyton.2023.028317

Abstract Banana is a tropical crop cultivated in warm places. Chilling stress in Egypt is making banana crops less productive. Abscisic acid (ABA), a key plant hormone, regulates metabolic and physiological processes and protects plants from a variety of stresses. In vitro growing banana shoots were pre-treated with ABA at four concentrations (0, 25, 50, and 100 mM) and chilled at 5°C for 24 h, followed by a six-day recovery period at 25°C. By comparing ABA treatments to both positive and negative controls, physiological and biochemical changes were investigated. Chilling stress (5°C) caused a considerable increase in lipid peroxidation and ion… More >