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  • Open Access

    ARTICLE

    Protocol for the reduction of costs in habanero chili (Capsicum chinense Jacq.) micropropagation

    Jimarez-Montiel MJ1, A Robledo-Paz1, VM Ordaz-Chaparro2, LI Trejo-Tellez2, JC Molina-Moreno1

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 94-104, 2018, DOI:10.32604/phyton.2018.87.094

    Abstract An alternative method for improving the production of habanero chili is the tissue culture technique; however, the gelling agent, the high salt and sucrose concentrations used in the culture media raise production costs and limit the adaptation of the regenerated plants to greenhouse or field conditions. In this study, the effect of the substrates perlite-coconut fiber, coconut fiber-volcanic rock, vermiculite-perlite, and perlite-volcanic rock in conjunction with various culture media in in vitro plant regeneration from embryos was evaluated. The differentiation of adventitious shoots on substrates was scarcely observed or non-existent. Inducing the formation of shoots on agar and their development… More >

  • Open Access

    ARTICLE

    In vitro antiviral activity and phytochemical screen in the extracts of peels from four species of tropical fruits collected in Merida Yucatan, Mexico

    Chel-Guerrero LD1, R Gómez-Cansino2, SL Gúzman-Gutierrez3, MG Campos-Lara4, E Saury-Duch1, F Díaz de León Sánchez5, R Reyes-Chilpa6, JA Mendoza-Espinoza7

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 68-71, 2018, DOI:10.32604/phyton.2018.87.068

    Abstract The objective of this study was to investigate extracts from the peels of Annona squamosa L. (purple sugar apple), Annona reticulata L. (custard apple), Chrysophyllum cainito L. (green star apple), and Melicoccus bijugatus Jacq. (mamoncillo), as potential sources of anti-HIV-1 agents. Methanolic extracts from the peels of the aforementioned tropical fruits were obtained by maceration. Antiviral activity was evaluated through HIV-1 RT inhibition by extracts, using a non-radioactive immuno/colorimetric assay (Lenti RT Activity Assay, Cavidi Tech). Assay was performed according to the protocol provided by the manufacturer. Extracts from the peels of A. squamosa, A. reticulata, and C. cainito showed… More >

  • Open Access

    ARTICLE

    Behavior and preservation of an in vitro collection of European aspen in Spain

    M.T. MARTIN*, H.E. PEDRANZANI**, R. SIERRA DE GRADO

    BIOCELL, Vol.31, No.1, pp. 41-49, 2007, DOI:10.32604/biocell.2007.31.041

    Abstract An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations.
    Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting… More >

  • Open Access

    ARTICLE

    Materials to Regulate Cell Fate In Vitro and In Vitro

    D. Mooney1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 169-169, 2006, DOI:10.32604/mcb.2006.003.169

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Trypanosoma rangeli: growth in mammalian cells in vitro and action of a repositioned drug (17-AAG) and a natural extract (Artemisia sp. essential oil)

    Ana Laura CIMADOR1,#, Emeli Luciana GALANTE1,#, Lucila Ibel MUÑOZ1,#, Patricia Silvia ROMANO2,3, Antonella Denisse LOSINNO1,2, María Cristina VANRELL1,2,3

    BIOCELL, Vol.43, No.1, pp. 13-20, 2019, DOI:10.32604/biocell.2019.07018

    Abstract Trypanosoma rangeli and T. cruzi are both parasitic unicellular species that infect humans. Unlike T. cruzi, the causative agent of Chagas disease, T. rangeli is an infective and non-pathogenic parasite for humans, but pathogenic for vectors from the Rhodnius genus. Because both species can coexist in different hosts and overlap their infective cycles but very little is known about the infection of T. rangeli in mammalian cells, we decided to characterize both the development of this parasite in cell culture and the effect of therapeutic agents with potential trypanocidal action on it. We found that T. rangeli exhibits a cycle… More >

  • Open Access

    ARTICLE

    In Vitro Measurement and Calculation of Drag Force on Iliac Limb Stentgraft in a Compliant Arterial Wall Model

    A. Sinha Roy*, K. West, R. S. Rontala1, R. K. Greenberg2, R. K. Banerje1,‡

    Molecular & Cellular Biomechanics, Vol.4, No.4, pp. 211-226, 2007, DOI:10.3970/mcb.2007.004.211

    Abstract Interventional treatment of aortic aneurysms using endovascular stentgrafting is a minimally invasive technique. Following device implantation, transient drag forces act on the stentgraft. When the drag force exceeds the fixation force, complications like stentgraft migration, endoleaks and stentgraft failure occur. In such a scenario the device becomes unstable, causing concern over the long-term durability of endovascular repairs. The objective of this study is: 1) to measure the drag force on iliac limb stentgraft, having a distal diameter that is half the size of the proximal end, in an in vitro experiment; 2) to calculate the drag force using blood flow--compliant… More >

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