SUSHMITHA SRIRAMULU1, SARUBALA MALAYAPERUMAL1, SUMAN K. NANDY2, ANTARA BANERJEE1, MUSTHAFA MOHAMED ESSA3,4, SARAVANABABU CHIDAMBARAM5, M. WALID QORONFLEH6,7, SURAJIT PATHAK1,*
BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756
Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to identify the possible interactions between… More >
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