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  • Open Access

    ARTICLE

    Butyrate inhibits the bovine rumen epithelial cell proliferation via downregulation of positive regulators at G0/G1 phase checkpoint

    KANG ZHAN#, MAOCHENG JIANG#, TIANYU YANG, ZIXUAN HU, GUOQI ZHAO*

    BIOCELL, Vol.46, No.7, pp. 1697-1704, 2022, DOI:10.32604/biocell.2022.019068

    Abstract Short-chain fatty acids (SCFAs) butyrate promote the postnatal rumen epithelial development and maturation in ruminants. However, molecular mechanisms of effects of butyrate on the bovine rumen epithelial cells (BRECs) proliferation remain elusive. Therefore, purpose of this study was to investigate the effects of butyrate on the expression of genes and proteins at G0/G1 and S phase of BRECs cycle. Our results showed that BRECs treated with butyrate inhibited (P < 0.05) the proliferation of BRECs, relatively to control. Flow cytometric assays revealed that butyrate triggers the BRECs cycle arrest at the G0/G1 phase. qRT-PCR analyses of mRNA level of genes… More >

  • Open Access

    ARTICLE

    Miscibility of Poly(hydroxybutyrate)/Poly(vinyl alcohol) Melt Blends Plasticized With Glycerol

    Deliane da Silva Cabral, Antonio José Felix Car

    Journal of Renewable Materials, Vol.7, No.4, pp. 325-333, 2019, DOI:10.32604/jrm.2019.01794

    Abstract Poly(hydroxybutyrate)/poly(vinyl alcohol) (PHB/PVA) blends plasticized with glycerol were prepared by melt blending of PHB and glycerol plasticized PVA. The PHB/PVA-glycerol compositions were 90:10, 75:25 and 50:50 w/w, being the concentration of glycerol in the PVA mixture of 10 wt %. The blends were characterized by infrared spectroscopy, dynamic-mechanical thermal analysis, and scanning electron microscope of the fragile fractured surface. The results showed one single phase blend, indicating miscibility corroborated by the presence of a single glass transition temperature. The blending method proved to be an efficient way to tune PHB properties keeping its biodegradable nature since both PVA and glycerol… More >

  • Open Access

    ARTICLE

    The Effects of Gamma Irradiation on Molecular Weight, Morphology and Physical Properties of PHBV/Cloisite 30B Bionanocomposites

    Kahina Iggui1,2,*, Mustapha Kaci1, Mohamed Mahlous3, Nicolas Le Moigne4, Anne Bergeret4

    Journal of Renewable Materials, Vol.7, No.9, pp. 807-820, 2019, DOI:10.32604/jrm.2019.06778

    Abstract In this paper, the effects of gamma irradiation on Cast poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and PHBV/Cloisite 30B (C30B) (3 wt%) bionanocomposite prepared by melt compounding, were evaluated at various doses, i.e., 5, 15, 20, 50 and 100 kGy at room temperature in air. Changes in molecular weight, morphology and physical properties were investigated. The study showed that the main degradation mechanism occurring in gamma irradiation in both Cast PHBV and C-PHBV/3C30B bionanocomposite is chain scission, responsible for the decrease of molecular weight. Differential scanning calorimetry (DSC) data indicated a regular decrease in crystallization temperature, melting temperature and crystallinity index for all irradiated… More >

  • Open Access

    ARTICLE

    Evaluation of Biodegradation in Aqueous Medium of Poly(Hydroxybutyrate-Co-Hydroxyvalerate)/Carbon Nanotubes Films in Respirometric System

    Larissa Stieven Montagna, Isabela CÉSAR Oyama, Rita de CÁSSIA Barbosa Camargo Lamparelli, Ana Paula Silva, THAÍS Larissa do Amaral Montanheiro, Ana Paula Lemes*

    Journal of Renewable Materials, Vol.7, No.2, pp. 117-128, 2019, DOI:10.32604/jrm.2019.00036

    Abstract Biodegradable polymers have been increasingly used for scientific and commercial applications because they are similar to some conventional thermoplastics and exhibit the ability of self-degradation. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanocomposites films with 1 and 2 wt% of carbon nanotubes (CNT) were prepared by solution mixing, followed by solvent evaporation. In this work, PHBV/CNT nanocomposites were submitted to biodegradation in an aqueous medium for 34 days through a respirometric system. Then, the PHBV films were analyzed by the CO2 production and mineralization as a response of a microbial attack, which was monitored by back titration during all the experiment. The films were also… More >

  • Open Access

    ARTICLE

    The Effects of Accelerated Photooxidation on Molecular Weight and Thermal and Mechanical Properties of PHBV/Cloisite 30B Bionanocomposites

    Kahina Iggui1,2,*,†, Mustapha Kaci2, Nicolas Le Moigne1, Anne Bergeret1

    Journal of Renewable Materials, Vol.6, No.3, pp. 288-298, 2018, DOI:10.7569/JRM.2017.634184

    Abstract The effects of accelerated photooxidation on the molecular weight and thermal and mechanical properties of Cast PHBV and PHBV/Cloisite 30B (3 wt%) bionanocomposites are investigated herein. Through size exclusion chromatography (SEC) analysis, a significant decrease in both weight and number average molecular weights was observed for all irradiated samples over time, resulting from the chain scission mechanism. Differential scanning calorimetry (DSC) data indicated a decrease in degree of crystallinity and melting temperature after UV exposure, with the appearance of double melting peaks related to the changes in the crystal structure of PHBV. Thermal stability, tensile and thermo-mechanical properties were also… More >

  • Open Access

    ARTICLE

    Production of Polyhydroxybutyrate (PHB) by Bacillus megaterium DSM 32 from Residual Glycerol of the Bioenergy Industry

    Enzo Alvarado-Cordero1, Gabriela Montes de Oca-Vásquez2, Reinaldo Pereira-Reyes2, José Vega-Baudrit2, Marianelly Esquivel-Alfaro1*

    Journal of Renewable Materials, Vol.5, No.3-4, pp. 323-331, 2017, DOI:10.7569/JRM.2017.634123

    Abstract Biodegradable polymers from renewable resources are generating growing interest in the plastic industry because they have properties similar to synthetic polymers. Polyhydroxyalkanoates, mainly polyhydroxybutyrate (PHB), have mechanical and physicochemical properties very similar to their synthetic counterparts. This work explores the use of residual glycerol from the bioenergy industry for the production of PHB by Bacillus megaterium DSM 32. The glycerol works as a source of carbon and energy. Raw glycerol was purified with sulfuric acid in order to neutralize saponified fatty acids. The purification process generated three different phases. One of the phases was the glycerol-rich layer; this layer was… More >

  • Open Access

    ARTICLE

    Mineralization of Poly(lactic acid) (PLA), Poly(3-hydroxybutyrate-co-valerate) (PHBV) and PLA/PHBV Blend in Compost and Soil Environments

    Sudhakar Muniyasamy1,2, Osei Ofosu1,2, Maya Jacob John1,2, Rajesh D. Anandjiwala1,2*

    Journal of Renewable Materials, Vol.4, No.2, pp. 133-145, 2016, DOI:10.7569/JRM.2016.634104

    Abstract The present study investigates the mineralization of poly(lactic acid) (PLA) and poly(3-hydroxybutyrate-covalerate) (PHBV), and PLA/PHBV blend in compost and soil burial environments. The mineralization was assayed on the basis of carbon dioxide (CO2) release from the test materials incubated in compost and soil for a period of 200 days. The degradation was followed by means of fragmentation, thermogravimetric (TGA), FTIR spectroscopy and scanning electron microscopy (SEM) analyses. The results showed that PLA, PHBV and blend of PLA/PHBV achieved almost 90% biodegradation under composting conditions, while PHBV, PLA/PHBV blend and PLA respectively achieved only 35%, 32% and 4% biodegradation under soil… More >

  • Open Access

    ARTICLE

    Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate)/ Purifi ed Cellulose Fiber Composites by Melt Blending: Characterization and Degradation in Composting Conditions

    Estefanía Lidón Sánchez-Safont1, Jennifer González-Ausejo1, José Gámez-Pérez1, José María Lagarón2, Luis Cabedo1*

    Journal of Renewable Materials, Vol.4, No.2, pp. 123-132, 2016, DOI:10.7569/JRM.2015.634127

    Abstract Novel biodegradable composites based on poly(3-hydroxybutirate-co-3-hydroxyvalerate) (PHBV) and different contents of purifi ed alpha-cellulose fi bers (3, 10, 25 and 45%) were prepared by melt blending and characterized. The composites were characterized by scanning electron microscopy (SEM), wide-angle X-ray scattering (WAXS) experiments, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic mechanic analysis (DMA) and Shore D hardness measurements. Disintegrability under composting conditions was studied according to the ISO 20200 standard. Morphological results showed that high dispersion of the fi bers was achieved during mixing. Good adhesion on the fi ber-matrix interface was also detected by SEM. The addition of low… More >

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