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Search Results (7)
  • Open Access

    ARTICLE

    Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells

    MIZUNA YANO1, KOTA HIROI1, TETSUYA YUASA1, KENJI INOUE1, OSAMU YAMAMOTO1, TAKAO NAKAMURA2, DAISUKE SATO1, ZHONGGANG FENG1,*

    BIOCELL, Vol.47, No.5, pp. 1095-1106, 2023, DOI:10.32604/biocell.2023.028186

    Abstract Background: Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period. Noticing the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells (hiPS-CM), researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of hiPS-CM. In our previous studies, we separately supplemented two polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) or arachidonic acid (AA), to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation, fatty acid metabolism, and cellular adhesion. The enhancement… More >

  • Open Access

    ARTICLE

    Mitochondria are an important target of photobiomodulation in cardiomyocytes

    XINLU GAO1,2,#, XIUXIU WANG1,2,#, WENWEN ZHANG1,2,#, HANJING LI1,2, FAN YANG2,3, WENYA MA2, YU LIU1,*

    BIOCELL, Vol.46, No.12, pp. 2637-2644, 2022, DOI:10.32604/biocell.2022.021033

    Abstract Photobiomodulation (PBM) has been shown to delay the pathological process of heart failure, but the exact mechanism of action is not clear. Mitochondria occupy one-third of the volume of mammalian cardiomyocytes (CMs) and are central transport stations for CM energy metabolism. Therefore, in this study, we explored the regulatory effects of 630 nm light-emitting diodes (LED-Red) on the mitochondria of CMs. The results show that LED-Red-based PBM promotes adenosine triphosphate (ATP) synthesis by upregulating the expression of glycolipid metabolizing enzymes. Correspondingly, there was an improvement in the activity of succinate dehydrogenase (SDH), a key enzyme in the mitochondrial electron transport… More >

  • Open Access

    ARTICLE

    Dihydroartemisinin ameliorates palmitate-induced apoptosis in cardiomyocytes via regulation on miR-133b/Sirt1 axis

    LONGJU QI1,2,#, XIAOYING XU3,#, BIN LI4,#, BO CHANG5, SHENGCUN WANG2, CHUN LIU2, LIUCHENG WU2, XIAODI ZHOU4, QINGHUA WANG2,*

    BIOCELL, Vol.46, No.4, pp. 989-998, 2022, DOI:10.32604/biocell.2022.018014

    Abstract Excessive fat ectopically deposited in the non-adipose tissues is considered as one of the leading causes of myopathy. The aim of this study was to investigate the role of Dihydroartemisinin (DHA) in palmitate (PAL)-incubated H9c2 cells (lipotoxicity-induced cell injury model). Cell viability of PAL-treated cells was determined by MTT assay, and apoptotic regulators were examined by qRT-PCR and western blot analysis, in the absence or in the presence of DHA, respectively. Expression levels of miR-133b and Sirt1 were also evaluated by qRT-PCR and western blotting examination. PAL decreased the viability of H9c2 cells and enhanced the expression of apoptotic genes.… More >

  • Open Access

    ARTICLE

    Effects of docosahexaenoic acid or arachidonic acid supplementation on gene expression and contractile force of rat cardiomyocytes in primary culture

    MIZUNA YANO1, YUTA UMEHARA1, TOMOKAZU KUDO1, TAKAO NAKAMURA2, TADASHI KOSAWADA1, ATSUYOSHI NISHINA3, MASAKI SAZUKA4, DAISUKE SATO1,*, ZHONGGANG FENG1,*

    BIOCELL, Vol.45, No.5, pp. 1213-1229, 2021, DOI:10.32604/biocell.2021.016281

    Abstract While fatty acids play essential roles in the physiology of the myocardium, conventional culture media contain little lipid. We previously revealed that rat neonatal myocardium mainly contains docosahexaenoic (DHA), linoleic (LA), and arachidonic (AA) acids as polyunsaturated fatty acids (PUFAs), and these contents in cultured cardiomyocytes derived from fetal rats were markedly lower than those in the neonatal myocardium. In this study, we first assessed the effects of supplementation of DHA, LA, or AA on the fatty acid contents and the percentage change of contractile area in primarily cultured rat cardiomyocytes. Based on this assessment, we then evaluated the effects… More >

  • Open Access

    ARTICLE

    miR-208a Promotes Apoptosis in H9c2 Cardiomyocytes by Targeting GATA4

    Liying Gong1,2,3, Hongkun Jiang4, Guangrong Qiu1, Kailai Sun1,*

    Congenital Heart Disease, Vol.16, No.5, pp. 499-512, 2021, DOI:10.32604/CHD.2021.015831

    Abstract Background: microRNAs are crucial for cardiovascular development and are associated with congenital heart disease (CHD). Recent studies have shown that microRNAs play a role in heart development and is closely related to CHD. The present study investigated the underlying mechanism of microRNA-208a (miR-208a) in “simple” CHD. Material and Methods: Reverse transcription-quantitative PCR (RT-qPCR) demonstrated miR-208a expression levels in children with CHD (n = 27) compared with normal controls (n = 29), in cardiomyocytes from embryo 10 (E10) to post-birth (P7) and organs in adult rats in healthy rats. Apoptosis of H9c2 cells after transfection with miR-208a detected by TUNEL assay.… More >

  • Open Access

    ARTICLE

    Upregulation of miR-143-3p attenuates oxidative stress-mediated cell ferroptosis in cardiomyocytes with atrial fibrillation by degrading glutamic-oxaloacetic transaminase 1

    YUAN SONG1,#, CAI WEI2,#, JINGJING WANG3,*

    BIOCELL, Vol.45, No.3, pp. 733-744, 2021, DOI:10.32604/biocell.2021.013236

    Abstract Oxidative stress-mediated cell death in cardiomyocytes contributes to the development of atrial fibrillation. However, the detailed mechanisms are still unclear. In the present study, we established atrial fibrillation models in mice. The cardiomyocytes were isolated from atrial fibrillation mice and normal mice and were cultured in vitro, respectively. The results showed that cell proliferation and viability in cardiomyocytes with atrial fibrillation were significantly lower than the cells from the normal mice. Consistently, atrial fibrillation cardiomyocytes were prone to suffer from apoptotic cell death. Also, the oxidative stress and ferroptosis-associated signatures were significantly increased in atrial fibrillation cardiomyocytes compared to normal… More >

  • Open Access

    ARTICLE

    YB-1 downregulation attenuates UQCRC1 protein expression level in H9C2 cells and decreases the mitochondrial membrane potential

    HUIFANG CHEN1,2, XIAOYING ZHOU2, ZONGHONG LONG2, XIANGLONG TANG2, HONG LI2,*

    BIOCELL, Vol.44, No.3, pp. 371-379, 2020, DOI:10.32604/biocell.2020.08893

    Abstract UQCRC1 is one of the 10 mitochondrial complex III subunits, this protein has a role in energy metabolism, myocardial protection, and neurological diseases. The upstream mechanism of the UQCRC1 protective effect on cardiomyocytes is currently unavailable. In order to explore the upstream molecules of UQCRC1 and elucidate the protective mechanism of UQCRC1 on cardiomyocytes in more detail, we focused on the nuclease-sensitive elementbinding protein 1 (YB-1). We hypothesized YB-1 acts as an upstream regulatory molecule of UQCRC1. This study found that YB-1 RNAi significantly reduces the expression of the UQCRC1 protein level (p < 0.05) and obviously decreases the mitochondrial… More >

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