WENJUAN XU¹, GUANGMING DAI¹, YIFEI LYU², YINING ZHANG¹, XIAOLIN TU^1,*

BIOCELL, Vol.46, No.4, pp. 1097-1104, 2022, DOI:10.32604/biocell.2022.017505

Abstract This study established a method for isolating large numbers of high-purity osteocytes from high-density bone. Bone fragments derived from mice tibia and femurs were alternately digested with type I collagenase and EDTA nine times, and the digested cells and bone chips (BC) were cultured, digested, and passaged when cells were fully grown. The types of cells obtained were identified by morphology, viable cell counts, alkaline phosphatase staining, and biochemical activity analyses, and specific osteocyte and osteoblast markers were evaluated by quantitative real-time polymerase chain reaction. Our results showed that among the cells obtained from the third digestion (fractions 7–9) of… More >

ODIN RAMIREZ-FERNANDEZ^1,2, ESMERALDA ZUÑIGA-AGUILAR^3,*

BIOCELL, Vol.46, No.2, pp. 325-338, 2022, DOI:10.32604/biocell.2022.016892

Abstract This review aims to offer a vision of the clinical reality of cell therapy today in intensive medicine. For this, it has been carried out a description of the properties, functions, and Mesenchymal Stem Cells (MSCS) sources to subsequently address the evidence in preclinical models and studies clinical trials with whole cells and models attributed to small extracellular vesicles (sEVs), nanoparticles made up of microvesicles secreted by cells with an effect on the extracellular matrix, and their impact as an alternative towards cell-free regenerative medicine. MSCs are cells that enhance the regenerative capacity which can be differentiated typically in different… More >

YAN PI1, KEJI JIANG2, RONG HOU¹, YIFU GONG³, JUAN LIN¹, XIAOFEN SUN¹, AND KEXUAN TANG^1,4*

BIOCELL, Vol.34, No.3, pp. 139-144, 2010, DOI:10.32604/biocell.2010.34.139

Abstract Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10- hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in… More >

DULCE MA. BARRADAS-DERMITZ^1,*, PATRICIA M. HAYWARD-JONES², MARTÍN MATA-ROSAS³, BEATRIZ PALMEROSSÁNCHEZ², OSCAR B. J. PLATAS-BARRADAS⁴, RODOLFO F. VELÁSQUEZ-TOLEDO²

BIOCELL, Vol.34, No.1, pp. 1-6, 2010, DOI:10.32604/biocell.2010.34.001

Abstract Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg’s B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 μM). In… More >

N.M. OCARINO*, A. BOZZI**, R.D.O. PEREIRA*, N.M. BREYNER**, V.L. SILVA*, P. CASTANHEIRA**, A.M. GOES**, R. SERAKIDES*

BIOCELL, Vol.32, No.2, pp. 175-183, 2008, DOI:10.32604/biocell.2008.32.175

Abstract 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them… More >

M.E. RÜTTLER, C.S. YANZÓN, M.J. CUITIÑO, N.F. RENNA, M.A. PIZARRO, A.M. ORTIZ.

BIOCELL, Vol.30, No.2, pp. 301-308, 2006, DOI:10.32604/biocell.2006.30.301

Abstract Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator (aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 (astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a… More >

Ying Li¹, Bin Xue², Wenxu Sun², Junhua Wu², Wenting Yu², Meng Qin², Wei Wang², Yi Cao^2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 147-147, 2019, DOI:10.32604/mcb.2019.07027

Abstract A key challenge in biomaterials research is to produce synthetic hydrogels that can replicate the diverse mechanical properties of the naturally occurring tissues for various biomedical applications, including tissue engineering, stem cell and cancer research, cell therapy, and immunomodulation. However, currently, the methods that can be used to control the mechanical properties of hydrogels are very limited and are mainly focused only on the elasticity of hydrogels. In this work, combining single molecule force spectroscopy, protein engineering and theoretical modeling, we show that synthetic protein hydrogels with predictable mechanical properties can be rationally designed using protein building blocks with known… More >

Odin RAMÍREZ-FERNÁNDEZ^1,*, Rafael GODÍNEZ¹, Esmeralda ZUÑIGA-AGUILAR¹, Luis E. GÓMEZ-QUIROZ², María C. GUTIÉRREZ-RUIZ², Juan MORALES³, Roberto OLAYO³

BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009

Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design and was tested with liver… More >

Juanita BUSTAMANTE^1,*, Analía CZERNICZYNIEC², Silvia LORES-ARNAIZ²

BIOCELL, Vol.40, No.1, pp. 11-14, 2016, DOI:10.32604/biocell.2016.40.011

Abstract The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine effect on the mitochondrial Ca²⁺ accumulation and the cellular Ca²⁺ mobilization using FLUO4-AM and flow cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased calcium influx through cellular depolarization. According to the… More >

Grisel Fierros-Romero^1,*, M. Elena Mellado-Rojas², Reynaldo C. Pless³, Juan Ignacio Valiente-Banuet¹, Ashutosh Sharma¹ and Elda M. Beltrán-Peña²

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 131-137, 2019, DOI:10.32604/phyton.2019.05036

Abstract Torin 1 is an ATP-competitive TOR inhibitor which inhibits the signaling of TOR and S6K kinase in mammals and plants. The objective of this research is to determine the effect of Torin 1 in a relatively simple and homogeneous plant system such as the NT-1 tobacco suspension cell cultures. Cultures of NT-1 cells were tested with 5, 50, 150 and 250 nM of Torin 1. During kinetics growth of NT-1 tobacco suspension cell cultures, 150 and 250 nM Torin 1 inhibits the early growth and later enhanced the cellular proliferation during exponential growth by means of an increased expression of… More >