DONGQIN WANG^1,4, YAN SHI¹, ZHIQIANG WANG⁴, JING ZHANG², LUYAO WANG², HONGYU MA³, SHUHUA SHI², XIAOFU LIAN², HUA HUANG⁴, XIAOJING WANG^1,*, CHAOQUN LIAN^4,*

BIOCELL, Vol.48, No.1, pp. 111-122, 2024, DOI:10.32604/biocell.2023.046903

Abstract Introduction: Among all malignant tumors of the digestive system, pancreatic carcinoma exhibits the highest mortality rate. Currently, prevention and effective treatment are urgent issues that need to be addressed. Methods: The study focused on meiotic nuclear divisions 1 (MND1), integrating data from the Gene Expression Profiling Interactive Analysis (GEPIA) database with prognostic survival analysis. Simultaneously, experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC. The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA (siRNA) in Patu-8988 and… More >

KANG SUN¹, DONGQIN WANG¹, ZHIQIANG ZHANG¹, YINLONG HUANG³, XIAOFU LIAN³, JIALE HUA³, JING ZHANG^3,*, CHAOQUN LIAN^1,2,*

BIOCELL, Vol.47, No.2, pp. 297-307, 2023, DOI:10.32604/biocell.2023.023553

Abstract Columbianetin acetate (CE) is one of the effective components of Angelica pubescens. So far, the specific role and molecular mechanism of CE in pancreatic cancer are not clear. Thus, this study aimed to explore the specific mechanism of CE on pancreatic cancer. The target genes combined with CE were predicted through the PharmMapper database and the 3D molecular structure of CE. Then, the Cancer Genome Atlas (TCGA) and Cistrome data browser (DB) databases were used to screen Meiotic nuclear divisions 1 (MND1)-related genes, transcription factors, and transcription factor data sets, and the intersection of the above data sets. The “limma”… More >

M. Vinoth Kumar¹, K. Venkatachalam², Mehedi Masud³, Mohamed Abouhawwash^4,5,*

Intelligent Automation & Soft Computing, Vol.33, No.3, pp. 1547-1559, 2022, DOI:10.32604/iasc.2022.023961

Abstract Huge data processing applications are stored efficiently using cloud computing platform. Few technologies like edge computing, Internet of Things (IoT) model helps cloud computing framework for executing data with less energy and latencies for better infrastructure. Recently researches focused on providing excellent services to cloud computing users. Also, cloud-based services are highly developed over IT field. Energy a level varies based on the cloud setup like speed, memory, service capability and bandwidth. The user job requirements are varied based its nature. The process of identifying efficient energy resources for the user job is main aim of this research work. Initially… More >

M.I. PIGOZZI, R.B. SCIURANO, A.J. SOLARI

BIOCELL, Vol.29, No.2, pp. 195-203, 2005, DOI:10.32604/biocell.2005.29.195

Abstract A testicular biopsy from an infertile man carrying a heterozygous chromosome translocation t(11;14) was studied with synaptonemal complex analysis and immunolocalization of the protein MLH1 for crossover detection. A full blockage of spermatogenesis at the spermatocyte stage was related to the presence of the translocation quadrivalents at pachytene. Only 2% of the quadrivalents showed full synapsis. Most of the spermatocytes showed asynaptic free ends that frequently mingled with the XY pair. The average number of crossovers per cell was diminished from a mean of 52.7 in controls to a mean of 48 in the patient. The difference between the number… More >

ROMINA J. BEVACQUA, RAFAEL FERNANDEZ-MARTIN, DANIEL F. SALAMONE

BIOCELL, Vol.35, No.1, pp. 1-7, 2011, DOI:10.32604/biocell.2011.35.001

Abstract Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment… More >

Hernández-Quintero JD¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, M Gómez-Martínez², R Rodríguez-Herrera⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 107-112, 2015, DOI:10.32604/phyton.2015.84.107

Abstract The genus Dasylirion is a group of plants typically present in the Chihuahuan Desert, perennial, with a dioecious sexual behavior and commonly called sotoles. This genus has been little studied from the biological point of view, and the bases of its reproductive response remain unknown. In this work we studied the chromosome number and meiotic response of Dasylirion cedrosanum in the county of Saltillo, Coahuila, located at the North East of Mexico. For the preparation of mitotic chromosomes, we used a technique based on enzymatic treatment with pectolyase and cellulase, as well as staining with acetocarmin dye. For the study… More >

Rodríguez de León JI¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, F Ramírez-Godina², V Robledo-Torres², M Gómez-Martínez², G Hernández-Guzmán⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 101-106, 2015, DOI:10.32604/phyton.2015.84.101

Abstract The cultivated husk tomato (Physalis ixocarpa) (2n = 2x = 24) is native from Mexico and Central America and shows a wide genetic variation. Presently, it is the fourth horticultural crop in cultivation surface in Mexico. The working team of this research previously developed an autotetraploid population by using colchicine. The objectives of the present work were to analyze the ploidy level and meiotic behavior of the subsequent generations (C3, C4, C5, C6) from the original (C2) composed only by plants with the duplicated genome from the Rendidora cultivar, and to determine pollen viability. As a diploid control the cultivar… More >

Gracieli DALLA NORA, Solange Bosio TEDESCO, Tamara PASTORI, Viviane Dal-Souto FRESCURA^*, Nerinéia Dalfollo RIBEIRO, Haywood Dail LAUGHINGHOUSE IV, Sidinei José LOPES, Maria Teresa SCHIFINO-WITTMANN

BIOCELL, Vol.38, No.1, pp. 33-38, 2014, DOI:10.32604/biocell.2014.38.033

Abstract The aims of this study were to determine the meiotic behavior and to estimate pollen grains viability in bean (Phaseolus vulgaris L.) cultivars. Flower buds were collected during different developmental stages of the Mesoamerican bean cultivars IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662 and Pérola, and the Andean bean cultivar Iraí, grown in a greenhouse. The meiotic index was determined by anther squashing of material fixed in absolute ethanol-glacial acetic acid (3:1) and stained with acetic orcein. No meiotic abnormalities were observed and the meiotic indices were high for all cultivars, indicating that the mismatch generated during… More >