Peng Guo^*, Guohui Zhang^*†, Jialin Meng^‡, Qian He^*, Zhihui Li^†, Yawei Guan^†

Oncology Research, Vol.26, No.7, pp. 1083-1091, 2018, DOI:10.3727/096504018X15152085755247

Abstract Bladder cancer (BC) is one of the leading causes of cancer-related deaths in the world. Long noncoding RNA (lncRNA) taurine-upregulated gene 1 (TUG1) plays an important role in the development and progression of numerous cancers, including BC. However, the exact role of TUG1 in modulating BC progression is still poorly known. In this study, we found that TUG1 was upregulated and microRNA-29c (miR-29c) was downregulated in BC tissues and cell lines. Overexpression of TUG1 promoted the cell proliferation of T24 and EJ cells, whereas TUG1 knockdown had the opposite effect. Upregulation of TUG1 obviously facilitated… More >

Zhixian Guo^*1, Jingjing Li^*1, Jihong Sun^†, Lu Sun^†, Yubing Zhou^‡, Zujiang Yu^*

Oncology Research, Vol.26, No.7, pp. 1073-1081, 2018, DOI:10.3727/096504017X15145088802439

Abstract Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. MicroRNA (miRNA), a class of noncoding single-stranded RNA molecules, is involved in regulating cancer cell proliferation, metastasis, migration, invasion, and apoptosis. We showed that the expression of miR-346 was significantly increased in HCC tissues and cell lines, compared with noncancerous controls, and was associated with poor prognosis. Overexpression of miR-346 promoted proliferation and inhibited apoptosis of SMMC-7721 cells, while knockdown of miR-346 significantly suppressed proliferation and induced apoptosis of HepG2 cells. Then we identified breast cancer metastasis suppressor 1 (BRMS1) as a direct More >

Xingquan Zhai, Wei Xu

Oncology Research, Vol.26, No.7, pp. 1063-1072, 2018, DOI:10.3727/096504018X15152072098476

Abstract This study aimed to explore the biological functions of long noncoding RNA activated by transforming growth factor-b (lncRNA-ATB) in bladder cancer cells. For the expressions of lncRNA-ATB, miR-126, and KRAS, T24 cells were transfected with their specific vectors/shRNA or mimic/inhibitor. Then cell viability, migration, invasion, and apoptosis as well as the protein levels of apoptosis-related factors and PI3K/AKT and mTOR signal pathways were measured. The relationships of lncRNA-ATB and miR-126 or miR-126 and KRAS were analyzed by Dual-Luciferase Reporter assay. Functional experiments showed that lncRNA-ATB overexpression significantly promoted cell viability, migration, and invasion in T24 More >

Lixia Jiang^*1, Shaohua Shi^†1, Qiaofa Shi^‡, Huijuan Zhang^*, Yu Xia^§, Tianyu Zhong^*

Oncology Research, Vol.26, No.7, pp. 1055-1062, 2018, DOI:10.3727/096504018X15152056890500

Abstract HIF-2α knockdown inhibits proliferation, arrests the cell cycle, and promotes apoptosis and autophagy under hypoxic conditions in cervical cancer. However, the upstream regulatory mechanism of HIF-2α expression is unclear. MicroRNAs (miRNAs) degrade target mRNAs by binding to the 3'-untranslated region of mRNAs. In this study, we investigated the role of miRNAs in the regulation of HIF-2α expression in cervical cancer under hypoxic conditions. miRNAs regulating HIF-2α expression were predicted using TargetScan and miRanda and were determined in cervical cancer under hypoxic conditions by qRT-PCR. Additionally, the targeted regulation of HIF-2α by miR-519d-3p was evaluated by… More >

Jian Ding^*, Fuqing Wang^†, Tiangang Xiang^‡, Meng Qiao^§

Oncology Research, Vol.26, No.7, pp. 1047-1053, 2018, DOI:10.3727/096504018X15152037713570

Abstract The aberrant expression of long noncoding RNAs (lncRNAs) is implicated in cancer development and progression. This study was aimed to investigate the expression and clinical significance of lncRNA NONHSAT129183 in papillary thyroid cancer (PTC), and to explore its roles in PTC cell proliferation, migration, and invasion. Our results demonstrate that lncRNA NONHSAT129183 is upregulated in human PTC tissues when compared with that in adjacent noncancerous thyroid tissue. Moreover, its expression is correlated with tumor size, lymph node metastasis, and TNM stage in PTC patients. lncRNA NONHSAT129183 silencing also significantly suppressed cell proliferation, migration, and invasion More >

Mao Guoping^*, Liu Ran^†, Qin Yanru^*

Oncology Research, Vol.26, No.7, pp. 1023-1029, 2018, DOI:10.3727/096504018X15151515028670

Abstract Recent studies have suggested that the dysregulation of microRNAs (miRNAs) plays a critical role in the progression of human cancers, including gastric cancer (GC). miR-143 had been reported to function as a tumor suppressor in GC. However, the exact molecular mechanism of how miR-143 participates in GC progression remains to be determined. In this present study, we revealed that the expression of miR-143 was significantly downregulated in human GC tissues and cell lines compared with normal tissues and a normal gastric epithelium cell line. In addition, upregulation of the expression of miR-143 in a GC More >

Wei Lei¹, Wang Kang¹, Yang Nan, Zhang Lei, Li Zhongdong, Li Demin, Sun Lei, Huang Hairong

Oncology Research, Vol.26, No.7, pp. 1015-1022, 2018, DOI:10.3727/096504018X15151486241153

Abstract This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as having NSCLC were enrolled in this study. The expression level of miR-200c in NSCLC tissues and cell lines was investigated using the quantitative real-time polymerase chain reaction (RT-qPCR) method. We found that the expression of miR- 200c was significantly reduced in NSCLC tissues and cell lines compared with normal lung tissues and the human bronchial epithelial cell line. Overexpression of miR-200c using the miR-200c mimic significantly… More >

Jianping Xu, Liguo Sun, Wei Sun, Jianhai Tian, Huaiyuan Guo

Oncology Research, Vol.26, No.7, pp. 997-1003, 2018, DOI:10.3727/096504017X15140544654946

Abstract To investigate the effect of Kim-1 on 786-0 cells in vivo and in vitro, several experiments such as quantitative real-time PCR, Western blot, MTT, colony formation, and flow cytometry were performed to evaluate the biological behavior of 786-0 cells treated with Kim-1 siRNA. Furthermore, the tumor xenograft model was applied to BALB/c nude mice to assess the effect of Kim-1 silencing. Lentivirus-mediated RNAi effectively silenced Kim-1 in 786-0 cells. Kim-1 knockdown significantly inhibited the proliferation and colony formation ability of 786-0 cells (p < 0.01). The cell cycle of 786-0 cells was arrested in the G₀/G₁ phase (p < More >

Xi Shi^*1, Xiao Xiao^†1, Na Yuan^*, Shili Zhang^*, Fukang Yuan^‡, Xiaohong Wang^§

Oncology Research, Vol.26, No.7, pp. 987-996, 2018, DOI:10.3727/096504017X15140534417184

Abstract Cervical cancer is the fourth most common malignancy among females worldwide. MicroRNA-379 (miR-379) is aberrantly expressed in multiple human cancer types. However, the expression pattern, roles, and detailed regulatory mechanisms of miR-379 in cervical cancer remain unknown. In this study, we found that miR-379 expression was downregulated in cervical cancer tissues and cell lines. Low miR-379 expression was correlated with International Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis, and distant metastasis. Additionally, miR-379 overexpression suppressed the proliferation and invasion of cervical cancer cells. Furthermore, V-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) More >

Wang Xiufen¹, Xie Yucui², Wang Jing³

Oncology Research, Vol.26, No.6, pp. 977-985, 2018, DOI:10.3727/096504017X15037506066252

Abstract THIS ARTICLE WAS WITHDRAWN BY THE PUBLISHERS IN OCTOBER 2020. More >