Xinyue Wang^1,#, Jing Xu^1,#, Qihong Gu¹, Dingxuan Tang¹, Huoyan Ji², Shaoqing Ju², Feng Wang^2,*, Lin Chen³, Ruoyu Yuan^2,*

Oncologie, Vol.24, No.3, pp. 513-526, 2022, DOI:10.32604/oncologie.2022.024373

Abstract Background: MicroRNAs (miRNAs) have been identified as promising novel biomarkers for cancer diagnosis and prognosis, especially for hepatocellular carcinoma (HCC). Nowadays, the expression level of miR-21 in serum samples is a diagnostic indicator for HCC diagnosis. Thus, the quantitative determination of miRNA concentration is of significance in clinical practice. It is particularly important to establish an analytical detection method for miR-21 in patient serum. Methods: The signal readout for miR-21 was based on the mass response of a reporter peptide using an isotope dilution mass spectrometry (MS) method in this work. To be more specific, miR-21 was biotinylated before being… More >

Kesheng Lin^1,2, Jiawen Zhou^1,2, Lijuan Han^1,2, Ning Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 285-296, 2023, DOI:10.32604/phyton.2022.023219

Abstract As a traditional herbal medicine, the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects. It is essential to develop an effective method for the determination of the major alkaloids in U. rhynchophylla. In this research, a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids (corynoxeine, isocorynoxeine, isorhynchophylline, rhynchophylline) in U. rhynchophylla. Isorhynchophylline was selected as the internal reference substance, the content of which was determined by the traditional external standard method. Relative correction factors (RCF) between isorhynchophylline and the… More >

Kuntal Das¹, Syed Mohammed Basheeruddin Asdaq^2,*, M. Saifulla Khan^1,*, Sravani Singirikonda¹, Abdulhakeem S. Alamri^3,4, Walaa F. Alsanie^3,4, Majid Alhomrani^3,4, Sreeharsha Nagaraja^5,6, Katharigatta N. Venugopala^5,7

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2349-2365, 2022, DOI:10.32604/phyton.2022.022379

Abstract The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence of flavonoid components in… More >

J. David García-Ortíz¹, Rebeca González-Centeno¹, María Alejandra Torres-Tapia², J. A. Ascacio-Valdés¹, José Espinoza-Velázquez², Raúl Rodríguez-Herrera^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 929-941, 2022, DOI:10.32604/phyton.2022.018368

Abstract Because of the growing worldwide demand for maize grain, new alternatives have been sought for breeding of this cereal, e.g., development of polyembryonic varieties, which agronomic performance could positively impact the grain yield per unit area, and nutritional quality. The objectives of this study were to (1) determine the phytochemicals present in the embryo and endosperm of grain from maize families with high, low, and null polyembryony frequency, which were planted at different locations, and (2) state the relationship between these compounds and seed germination. The extracted phytochemicals from corn were identified by HPLC-MS. The results showed that the genotype… More >

POOJA BHADRECHA¹, MADHU BALA^2,3, VIKAS KAUSHIK⁴, NASEEM A. GAUR⁵, SIMRANJEET SINGH⁴, JOGINDER SINGH^4,*, MANOJ KUMAR^6,*

BIOCELL, Vol.45, No.2, pp. 387-394, 2021, DOI:10.32604/biocell.2021.013824

Abstract Hippophae rhamnoides L. is a plant of immense ethnopharmacological importance and is a known source for various valuable biochemicals and nutraceuticals. The production of folate, a vitamin involved in several vital functions, in this plant is rather poorly understood. Herein, we investigate the hypothesis that rhizobial bacteria serve the plant in this essential vitamin’s biosynthesis. Bacterial strains of Bacillus, Azorhizobium, Frankia, Paenibacillus, Brevibacillus and Pseudomonas, were isolated from the rhizosphere of the plant. HPLC and LCMS were used to trace the production of intra and extra-cellular folate by representative rhizospheric bacterial strains in vitro. From the seventeen functionally characterized bacterial… More >

Anna Stepanova^1,*, Maria Malunova¹, Svetlana Salamaikina¹, Renat Selimov², Aleksandra Solov’eva¹

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More >

A. Bikoro Bi Athomo^1,2, S. P. Engozogho Anris^1,2, R. Safou Tchiama^2,3, F. Eyma⁴, C. Arnaudguilhem⁵, B. Charrier^1,*

Journal of Renewable Materials, Vol.9, No.1, pp. 35-48, 2021, DOI:10.32604/jrm.2021.013626

Abstract

A complementary approach using Liquid Chromatographic-Mass Spectrometric analysis was proposed to characterize phenolic compounds from the methanol-water extracts of K. ivorensis A. Chev. Two High Performance Liquid Chromatography (HPLC) complementary methods were used for the determination of phenolic compounds from the bark, sapwood and heartwood of K. ivorensis. Methods employed involved direct analysis after filtration at 0.20 µm, using a RP C₁₈ column and UV-VIS/ESI-FTMS detection. The methods used were different by their elution gradient and allowed analyzing the chemical composition of three parts of African mahogany extracts. In this study, 22 phenolic compounds and derivatives from K. ivorensis were… More >

Arnab Kumar De, Arijit Ghosh, Debabrata Dolui, Indraneel Saha, Malay Kumar Adak^*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 999-1017, 2020, DOI:10.32604/phyton.2020.010828

Abstract In the present experiment with ongoing concentration (0 µM, 100 µM, 250 µM, 500 µM and 1000 µM) of 2,4-D, the responses of Azolla pinnata R.Br. was evaluated based on cellular functions. Initially, plants were significantly tolerated up to 1000 µM of 2,4-D with its survival. This was accompanied by a steady decline of indole acetic acid (IAA) concentration in tissues with 78.8% over the control. Membrane bound H⁺ -ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator (KCl) and decreased within a range of 57.3 to 74.6% in response to inhibitor (Vanadate) application.… More >

YAN PI1, KEJI JIANG2, RONG HOU¹, YIFU GONG³, JUAN LIN¹, XIAOFEN SUN¹, AND KEXUAN TANG^1,4*

BIOCELL, Vol.34, No.3, pp. 139-144, 2010, DOI:10.32604/biocell.2010.34.139

Abstract Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10- hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in… More >

González MD, CM Luis, PL Lanzelotti

Phyton-International Journal of Experimental Botany, Vol.83, pp. 179-184, 2014, DOI:10.32604/phyton.2014.83.179

Abstract Characterization of oregano in Argentina is limited. They are normally known with fancy names, but it is not possible to recognize whether they are genetically equivalent or not. This paper presents a non-volatile polyphenol study of one of the subspecies of Origanum vulgare, O. vulgare L. ssp. viridulum (Martrin-Donos) Nyman [= O. vulgare L. ssp. virens (Hoffmannsegg et Link) Ietswart]. The polyphenols 3-(3,4-dihydroxyphenyl)lactic acid, 3,4-dihydroxybenzoic acid, caffeic acid, 4-(3.4-dihydroxybenzoyloxymethyl)phenyl β-glucoside and rosmarinic acid were isolated and identified by spectroscopic analysis from leaves and inflorescences of classified material. In addition, the polyphenol profile of the hydroalcoholic extract of this oregano was… More >