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  • Open Access


    A UHPLC/MS/MS Assay Based on an Isotope-Labeled Peptide for Sensitive miR-21 Detection in HCC Serum

    Xinyue Wang1,#, Jing Xu1,#, Qihong Gu1, Dingxuan Tang1, Huoyan Ji2, Shaoqing Ju2, Feng Wang2,*, Lin Chen3, Ruoyu Yuan2,*

    Oncologie, Vol.24, No.3, pp. 513-526, 2022, DOI:10.32604/oncologie.2022.024373

    Abstract Background: MicroRNAs (miRNAs) have been identified as promising novel biomarkers for cancer diagnosis and prognosis, especially for hepatocellular carcinoma (HCC). Nowadays, the expression level of miR-21 in serum samples is a diagnostic indicator for HCC diagnosis. Thus, the quantitative determination of miRNA concentration is of significance in clinical practice. It is particularly important to establish an analytical detection method for miR-21 in patient serum. Methods: The signal readout for miR-21 was based on the mass response of a reporter peptide using an isotope dilution mass spectrometry (MS) method in this work. To be more specific, miR-21… More >

  • Open Access


    Rapid Analysis of Four Alkaloids in Uncaria rhynchophylla by Core-Shell Column HPLC and Quantitative Analysis of Multi-Components by Single Marker (QAMS)

    Kesheng Lin1,2, Jiawen Zhou1,2, Lijuan Han1,2, Ning Li1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 285-296, 2023, DOI:10.32604/phyton.2022.023219

    Abstract As a traditional herbal medicine, the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects. It is essential to develop an effective method for the determination of the major alkaloids in U. rhynchophylla. In this research, a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids (corynoxeine, isocorynoxeine, isorhynchophylline, rhynchophylline) in U. rhynchophylla. Isorhynchophylline was selected as the internal reference substance, the content of which was determined by the traditional external standard method. Relative correction factors (RCF) between isorhynchophylline More >

  • Open Access


    Phytochemical Analysis, Estimation of Quercetin, and in Vitro Anti-Diabetic Potential of Stevia Leaves Samples Procured from Two Geographical Origins

    Kuntal Das1, Syed Mohammed Basheeruddin Asdaq2,*, M. Saifulla Khan1,*, Sravani Singirikonda1, Abdulhakeem S. Alamri3,4, Walaa F. Alsanie3,4, Majid Alhomrani3,4, Sreeharsha Nagaraja5,6, Katharigatta N. Venugopala5,7

    Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2349-2365, 2022, DOI:10.32604/phyton.2022.022379

    Abstract The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence… More >

  • Open Access


    Embryo and Endosperm Phytochemicals from Polyembryonic Maize Kernels and Their Relationship with Seed Germination

    J. David García-Ortíz1, Rebeca González-Centeno1, María Alejandra Torres-Tapia2, J. A. Ascacio-Valdés1, José Espinoza-Velázquez2, Raúl Rodríguez-Herrera1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 929-941, 2022, DOI:10.32604/phyton.2022.018368

    Abstract Because of the growing worldwide demand for maize grain, new alternatives have been sought for breeding of this cereal, e.g., development of polyembryonic varieties, which agronomic performance could positively impact the grain yield per unit area, and nutritional quality. The objectives of this study were to (1) determine the phytochemicals present in the embryo and endosperm of grain from maize families with high, low, and null polyembryony frequency, which were planted at different locations, and (2) state the relationship between these compounds and seed germination. The extracted phytochemicals from corn were identified by HPLC-MS. The… More >

  • Open Access


    Folate-producing rhizobacteria of Hippophae rhamnoides L. from Indian trans-Himalaya low atmospheric zone


    BIOCELL, Vol.45, No.2, pp. 387-394, 2021, DOI:10.32604/biocell.2021.013824

    Abstract Hippophae rhamnoides L. is a plant of immense ethnopharmacological importance and is a known source for various valuable biochemicals and nutraceuticals. The production of folate, a vitamin involved in several vital functions, in this plant is rather poorly understood. Herein, we investigate the hypothesis that rhizobial bacteria serve the plant in this essential vitamin’s biosynthesis. Bacterial strains of Bacillus, Azorhizobium, Frankia, Paenibacillus, Brevibacillus and Pseudomonas, were isolated from the rhizosphere of the plant. HPLC and LCMS were used to trace the production of intra and extra-cellular folate by representative rhizospheric bacterial strains in vitro. From the seventeen functionally characterized bacterial More >

  • Open Access


    Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds

    Anna Stepanova1,*, Maria Malunova1, Svetlana Salamaikina1, Renat Selimov2, Aleksandra Solov’eva1

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

    Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS).… More >

  • Open Access


    Identification of Phenolic Compounds from K. ivorensis by Selected Chromatographic and Spectrometric Techniques

    A. Bikoro Bi Athomo1,2, S. P. Engozogho Anris1,2, R. Safou Tchiama2,3, F. Eyma4, C. Arnaudguilhem5, B. Charrier1,*

    Journal of Renewable Materials, Vol.9, No.1, pp. 35-48, 2021, DOI:10.32604/jrm.2021.013626


    A complementary approach using Liquid Chromatographic-Mass Spectrometric analysis was proposed to characterize phenolic compounds from the methanol-water extracts of K. ivorensis A. Chev. Two High Performance Liquid Chromatography (HPLC) complementary methods were used for the determination of phenolic compounds from the bark, sapwood and heartwood of K. ivorensis. Methods employed involved direct analysis after filtration at 0.20 µm, using a RP C18 column and UV-VIS/ESI-FTMS detection. The methods used were different by their elution gradient and allowed analyzing the chemical composition of three parts of African mahogany extracts. In this study, 22 phenolic compounds and derivatives from K. ivorensis

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  • Open Access


    2,4-D Hyper Accumulation Induced Cellular Responses of Azolla pinnata R. Br. to Sustain Herbicidal Stress

    Arnab Kumar De, Arijit Ghosh, Debabrata Dolui, Indraneel Saha, Malay Kumar Adak*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 999-1017, 2020, DOI:10.32604/phyton.2020.010828

    Abstract In the present experiment with ongoing concentration (0 µM, 100 µM, 250 µM, 500 µM and 1000 µM) of 2,4-D, the responses of Azolla pinnata R.Br. was evaluated based on cellular functions. Initially, plants were significantly tolerated up to 1000 µM of 2,4-D with its survival. This was accompanied by a steady decline of indole acetic acid (IAA) concentration in tissues with 78.8% over the control. Membrane bound H+ -ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator (KCl) and decreased within a range of 57.3 to 74.6% in response to… More >

  • Open Access


    Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments


    BIOCELL, Vol.34, No.3, pp. 139-144, 2010, DOI:10.32604/biocell.2010.34.139

    Abstract Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10- hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca More >

  • Open Access


    Polyphenolic profile of Origanum vulgare L. ssp. viridulum from Argentina

    González MD, CM Luis, PL Lanzelotti

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 179-184, 2014, DOI:10.32604/phyton.2014.83.179

    Abstract Characterization of oregano in Argentina is limited. They are normally known with fancy names, but it is not possible to recognize whether they are genetically equivalent or not. This paper presents a non-volatile polyphenol study of one of the subspecies of Origanum vulgare, O. vulgare L. ssp. viridulum (Martrin-Donos) Nyman [= O. vulgare L. ssp. virens (Hoffmannsegg et Link) Ietswart]. The polyphenols 3-(3,4-dihydroxyphenyl)lactic acid, 3,4-dihydroxybenzoic acid, caffeic acid, 4-(3.4-dihydroxybenzoyloxymethyl)phenyl β-glucoside and rosmarinic acid were isolated and identified by spectroscopic analysis from leaves and inflorescences of classified material. In addition, the polyphenol profile of the hydroalcoholic extract of this… More >

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