Open Access

ARTICLE

HPLC-DAD Profiling and Diuretic Effect of Solanum elaeagnifolium (Cav.) Aqueous Extract: A Combined Experimental and Computational Approach

Bouslamti Mohammed¹, Nouioura Ghizlane¹, Tbatou Widad¹, Mohamed Chebaibi^2,3, Abdulsalam Alhalmi⁴, Najoua Soulo¹, Sulaiman Mohammed Alnasser⁵, Fahad M Alshabrmi⁶, Amine Elbouzidi^7,*, Lyoussi Badiaa¹, Benjelloun Ahmed Samir¹

1 Laboratory of Biotechnology, Conservation and Valorisation of Bioresources (BCVB), Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, 30000, Morocco
2 Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy of Fez, Sidi Mohamed Ben Abdellah University, Fez, 30000, Morocco
3 Ministry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Fez, 30000, Morocco
4 Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, 110062, India
5 Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Qassim, 51452, Saudi Arabia
6 Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, 51452, Saudi Arabia
7 Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco

* Corresponding Author: Amine Elbouzidi. Email:

(This article belongs to the Special Issue: Innovative Strategies in Medicinal Plant Biotechnology: From Traditional Knowledge to Modern Applications)

Phyton-International Journal of Experimental Botany 2025, 94(5), 1505-1518. https://doi.org/10.32604/phyton.2025.063896

Received 27 January 2025; Accepted 21 April 2025; Issue published 29 May 2025

Abstract

The Solanum genus is known for its diverse bioactive compounds, yet its diuretic potential remains understudied. This research commenced with an analysis of polyphenol and flavonoid content in Solanum elaeagnifolium leaf extract (SEFE) using colorimetric techniques, followed by HPLC-DAD to delineate its chemical composition. The aqueous extract revealed prominent constituents: naringin (12.38%), quercetin 3-O-B-D-Glucoside (27.25%), and flavone (15.26%). A 15-day study on normal rats investigated the diuretic potential of SEFE at repeated doses. SEFE significantly increased urine volume and urinary sodium/potassium levels without inducing hypokalaemia, contrasting with furosemide, a standard diuretic that induced hypokalaemia. Conversely, furosemide, a standard diuretic, increased urinary sodium and potassium while inducing hypokalaemia. It was evident that the diuretic effect of S. elaeagnifolium is dose-dependent, with a dosage of 500 mg/kg body weight exerting a more potent diuretic effect compared to furosemide. The diuretic activity of this plant was supported by an in silico study of the diuretic effect. The findings demonstrate how S. elaeagnifolium leaves have a potent diuretic impact on rats. However, more in-depth studies are needed to examine the following aspects: identifying the specific molecules responsible for the diuretic effect, understanding the molecular and cellular mechanisms underlying this activity, and assessing the long-term safety and clinical efficacy of this plant in different contexts.

---

Keywords

---