Open Access

ARTICLE

Rapid Analysis of Four Alkaloids in Uncaria rhynchophylla by Core-Shell Column HPLC and Quantitative Analysis of Multi-Components by Single Marker (QAMS)

Kesheng Lin^1,2, Jiawen Zhou^1,2, Lijuan Han^1,2, Ning Li^1,2,*

1 KMHD (Kangmeihuada Gene Technology Co., Ltd.), Shenzhen, 518128, China
2 Guangdong Province Enterprise Key Laboratory of Traditional Chinese Medicine Decoction Pieces, Kangmei Pharmaceutical Co., Ltd., Jieyang, 515300, China

* Corresponding Author: Ning Li. Email:

(This article belongs to this Special Issue: Therapeutic Efficiency of Phyto-Molecules)

Phyton-International Journal of Experimental Botany 2023, 92(1), 285-296. https://doi.org/10.32604/phyton.2022.023219

Received 15 April 2022; Accepted 20 May 2022; Issue published 06 September 2022

Abstract

As a traditional herbal medicine, the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects. It is essential to develop an effective method for the determination of the major alkaloids in U. rhynchophylla. In this research, a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids (corynoxeine, isocorynoxeine, isorhynchophylline, rhynchophylline) in U. rhynchophylla. Isorhynchophylline was selected as the internal reference substance, the content of which was determined by the traditional external standard method. Relative correction factors (RCF) between isorhynchophylline and the other three alkaloids were calculated respectively. The results showed that the QAMS method had good robustness under different HPLC instruments. Nineteen batches of U. rhynchophylla were tested. No significant difference was observed between the results by QAMS and EMS (Correlation coefficient > 0.99, p > 0.05). The QAMS method could be employed as a rapid, effective technique for the quality control of U. rhynchophylla.

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Keywords

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