Open Access
ARTICLE
Polyphenolic Compounds and Antioxidant Activity of Sea Buckthorn (Hippophae rhamnoides L.)
Ain Raal1,*, Linda Rusalepp2, Tatiana Chiru3, Nicolae Ciobanu3, Kelly Talvistu1, Michelle Shusta1, Oleh Koshovyi1,4, Tõnu Püssa2
1
Institute of Pharmacy, Faculty of Medicine, University of Tartu, Tartu, 50411, Estonia
2
Chair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of
Life Sciences, Tartu, 51006, Estonia
3
Faculty of Pharmacy, Nicolae Testemitanu State Medical and Pharmaceutical University, Chisinau, MD-2001, Republic of Moldova
4
Pharmacognosy Department, The National University of Pharmacy, Kharkiv, 61002, Ukraine
* Corresponding Author: Ain Raal. Email:
(This article belongs to the Special Issue: Extraction Techniques, Composition and Bioactivity Research of Plant Extracts)
Phyton-International Journal of Experimental Botany 2023, 92(11), 2965-2979. https://doi.org/10.32604/phyton.2023.042723
Received 09 June 2023; Accepted 06 August 2023; Issue published 24 October 2023
Abstract
The fruits of the Sea buckthorn (
Hippophae rhamnoides L.) are a popular plant food and a valuable health product. Cultivating plants produces a lot of leaves with fine branches as an unnecessary residue, which needs valorization. The aim of the study was to estimate (by HPLC-MS/MS) the qualitative and quantitative content of
polyphenolic compounds in different parts of
H. rhamnoides (
HR), and to determine the level of antioxidant
activity of leaves and fruits (by DPPH, ABTS methods and ferrozine test). Up to 19 compounds were identified
in different parts of
HR. The fruits are rich in flavonoids, including various glycosides of flavonols isorhamnetin,
quercetin, and kaempferol. Two isorhamnetin glycosides were not identified in the leaves, while isorhamnetin-3-
rhamnosylglactosides and the ellagitannins hippophaenin B, stachyurin and casuarinin were present only in the
leaves of the plant. The bark and roots contained considerably more catechins, but minimal flavonols than the
fruits and leaves of
HR. The total phenolics and flavonols were most abundant in water infusions from leaves
of
HR (especially quercetin-3-O-glucoside-7-O-rhamnoside), compared to methanolic extracts. No significant differences in the quantitative and qualitative content of the fresh and dried leaves were detected. The highest antioxidant activity by all three methods was detected in the leaves of
HR. In addition to the fruits, the leaves may
have a perspective as a source of biologically active substances of
HR.
Keywords
Cite This Article
Raal, A., Rusalepp, L., Chiru, T., Ciobanu, N., Talvistu, K. et al. (2023). Polyphenolic Compounds and Antioxidant Activity of Sea Buckthorn (
Hippophae rhamnoides L.).
Phyton-International Journal of Experimental Botany, 92(11), 2965–2979. https://doi.org/10.32604/phyton.2023.042723