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  • Open Access

    ARTICLE

    Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng C. A. Mey.

    Lei Zhu1,#, Lihe Hou1,3,#, Yu Zhang1, Yang Jiang1, Yi Wang1,2, Meiping Zhang1,2, Mingzhu Zhao1,2,*, Kangyu Wang1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 313-327, 2024, DOI:10.32604/phyton.2024.047938

    Abstract Panax ginseng C. A. Mey. is an important plant species used in traditional Chinese medicine, whose primary active ingredient is a ginsenoside. Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes. Nonetheless, the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites. Squalene epoxidase (SQE) is a key enzyme in the mevalonic acid pathway, which affects the biosynthesis of secondary metabolites such as terpenoid. Using ginseng transcriptome, expression, and ginsenoside content databases, this study employed bioinformatic methods to systematically analyze the genes encoding… More >

  • Open Access

    ARTICLE

    Involvement of the ABA- and H2O2-Mediated Ascorbate–Glutathione Cycle in the Drought Stress Responses of Wheat Roots

    Mengyuan Li1, Zhongye Gao1,2, Lina Jiang1, Leishan Chen1,2,*, Jianhui Ma1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 329-342, 2024, DOI:10.32604/phyton.2024.046976

    Abstract Abscisic acid (ABA), hydrogen peroxide (H2O2) and ascorbate (AsA)–glutathione (GSH) cycle are widely known for their participation in various stresses. However, the relationship between ABA and H2O2 levels and the AsA–GSH cycle under drought stress in wheat has not been studied. In this study, a hydroponic experiment was conducted in wheat seedlings subjected to 15% polyethylene glycol (PEG) 6000–induced dehydration. Drought stress caused the rapid accumulation of endogenous ABA and H2O2 and significantly decreased the number of root tips compared with the control. The application of ABA significantly increased the number of root tips, whereas the application of H2O2 markedly… More >

  • Open Access

    ARTICLE

    The Effect of Soil Enzymes and Polysaccharides Secreted by the Roots of Salvia miltiorrhiza Bunge under Drought, High Temperature, and Nitrogen and Phosphorus Deficits

    Yong Qin1,2, Xiaoyu Li1,2, Yanhong Wu1,2, Hai Wang3, Guiqi Han1,2,3, Zhuyun Yan1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.1, pp. 119-135, 2024, DOI:10.32604/phyton.2023.046075

    Abstract Root exudates serve as crucial mediators for information exchange between plants and soil, and are an important evolutionary mechanism for plants’ adaptation to environmental changes. In this study, 15 different abiotic stress models were established using various stress factors, including drought (D), high temperature (T), nitrogen deficiency (N), phosphorus deficiency (P), and their combinations. We investigated their effects on the seedling growth of Salvia miltiorrhiza Bunge and the activities of Solid-Urease (S-UE), Solid-Nitrite Reductase (S-NiR), Solid-Nitrate Reductase (S-NR), Solid-Phosphotransferase (S-PT), and Solid-Catalase (S-CAT), as well as the contents of polysaccharides in the culture medium. The results showed that the growth… More >

  • Open Access

    REVIEW

    A Narrative Review Unravelling the Historical Roots of Myths Surrounding Hypnosis and Discerning Facts

    Revue narrative pour démêler les racines historiques des mythes entourant l’hypnose et discerner les faits

    Dali Geagea*

    Psycho-Oncologie, Vol.17, No.4, pp. 215-224, 2023, DOI:10.32604/po.2023.043338

    Abstract Hypnosis, one of the earliest mind-body treatments, has been the subject of renewed interest in the last decade with research indicating its effectiveness for varied physiological and psychological ailments including pain, distress, and psychosomatic disorders. However, the adoption and acceptance of hypnosis are still hindered by myths and misconceptions that prevail among the general public and clinicians. Considering their significant role in promoting the successful delivery and acceptance of interventions, informing clinicians about hypnosis is crucial to promote balanced views, and thereby the adoption of this intervention when warranted. This narrative review traces the historical roots of myths surrounding hypnosis,… More >

  • Open Access

    ARTICLE

    Polyphenolic Compounds and Antioxidant Activity of Sea Buckthorn (Hippophae rhamnoides L.)

    Ain Raal1,*, Linda Rusalepp2, Tatiana Chiru3, Nicolae Ciobanu3, Kelly Talvistu1, Michelle Shusta1, Oleh Koshovyi1,4, Tõnu Püssa2

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2965-2979, 2023, DOI:10.32604/phyton.2023.042723

    Abstract The fruits of the Sea buckthorn (Hippophae rhamnoides L.) are a popular plant food and a valuable health product. Cultivating plants produces a lot of leaves with fine branches as an unnecessary residue, which needs valorization. The aim of the study was to estimate (by HPLC-MS/MS) the qualitative and quantitative content of polyphenolic compounds in different parts of H. rhamnoides (HR), and to determine the level of antioxidant activity of leaves and fruits (by DPPH, ABTS methods and ferrozine test). Up to 19 compounds were identified in different parts of HR. The fruits are rich in flavonoids, including various glycosides… More >

  • Open Access

    ARTICLE

    In Vitro and in Silico Insights on the Biological Activities, Phenolic Compounds Composition of Hypericum perforatum L. Hairy Root Cultures

    Oliver Tusevski1,*, Marija Todorovska1, Jasmina Petreska Stanoeva2, Marina Stefova2, Sonja Gadzovska Simic1

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 921-941, 2023, DOI:10.32604/phyton.2023.024995

    Abstract Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed to… More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analysis of Salt-Stress-Responsive Genes in Rice Roots

    Rui Song1, Yan Huang2, Xin Ji3, Yunfei Wei3, Qiuyuan Liu3, Shumei Li3, Juan Liu3,*, Pengfei Dong1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 237-250, 2023, DOI:10.32604/phyton.2022.023081

    Abstract Soil salinity greatly impairs plant growth and crop productivity. Rice (Oryza sativa L.) is a salt-sensitive crop. To better understand the molecular mechanisms of salt tolerance in roots, the BGISEQ-500 sequencing platform was employed to elucidate transcriptome changes in rice roots after 0, 3, 24, and 72 h of salt stress. The results showed that root K+ content decreased and Na+ content increased rapidly after the initial stage of salt stress, but that fresh and dry weight in root did not significantly reduce. Compared to the control (no salt stress), 1,292, 453, and 486 differentially expressed genes (DEGs) were upregulated,… More >

  • Open Access

    ARTICLE

    T-Shaped Transmission Line Fault Location Based on Phase-Angle Jump Checking

    Jia’an Xie1,*, Yurong Wang2, Guobin Jin3, Mucheng Wu1

    Energy Engineering, Vol.119, No.5, pp. 1797-1809, 2022, DOI:10.32604/ee.2022.020344

    Abstract In order to effectively solve the dead-zone and low-precision of T-shaped transmission line fault location, a new T-shaped transmission line fault location algorithm based on phase-angle jump checking is proposed in this paper. Firstly, the 3-terminal synchronous fundamental positive sequence voltage and current phasors are extracted and substituted into the fault branch distance function to realize the selection of fault branch when the fault occurs; Secondly, use the condition of the fundamental positive sequence voltage phasor at the fault point is equal to calculate all roots (including real root and virtual roots); Finally, the phase-angle jump check function is used… More >

  • Open Access

    ARTICLE

    Participation of Auxin Transport in the Early Response of the Arabidopsis Root System to Inoculation with Azospirillum brasilense

    Elizabeth Carrillo-Flores1, Jonanci Arreola-Rivera1, Denní Mariana Pazos-Solís2, Moisés Bocanegra-Mondragón2, Grisel Fierro-Romero3, Ma. Elena Mellado-Rojas1, Elda Beltrán-Peña1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2383-2401, 2022, DOI:10.32604/phyton.2022.021507

    Abstract The potential of Plant Growth Promoting Rhizobacteria (PGPR) has been demonstrated in the case of plant inoculation with bacteria of the genus Azospirillum which improves yield. A. brasilense produces a wide variety of molecules, including the natural auxin indole-3-acetic acid (IAA), as well as other phytoregulators. However, several studies have suggested that auxin induces changes in plant development during their interaction with the bacteria. The effects of A. brasilense Sp245 on the development of Arabidopsis thaliana root were investigated to help explain the molecular basis of the interaction. The results obtained showed a decrease in primary root length from the… More >

  • Open Access

    ARTICLE

    Exogenous melatonin alleviated growth inhibition and oxidative stress induced by drought stress in apple rootstock

    MEIGE WANG, JUAN GONG, CHUNHUI SONG, ZHENGYANG WANG, SHANGWEI SONG, JIAN JIAO, MIAOMIAO WANG, XIANBO ZHANG*, TUANHUI BAI*

    BIOCELL, Vol.46, No.7, pp. 1763-1770, 2022, DOI:10.32604/biocell.2022.018934

    Abstract

    Drought stress is one of the major environmental obstacles that limit the production and development of apples (Malus domestica Borkh.). The role of melatonin is well known in the protection of plants under environmental stresses. In this study, we investigated the effect of melatonin on apple rootstock M. hupehensis Rehd under drought stress. The results showed that drought inhibited the growth of M. hupehensis and dramatically reduced root surface area, root volume, the number of tips and forks, and root diameter. Drought-induced growth inhibition was significantly decreased by adding melatonin. Net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomatal conductance… More >

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