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  • Open Access

    ARTICLE

    In Vitro and in Silico Insights on the Biological Activities, Phenolic Compounds Composition of Hypericum perforatum L. Hairy Root Cultures

    Oliver Tusevski1,*, Marija Todorovska1, Jasmina Petreska Stanoeva2, Marina Stefova2, Sonja Gadzovska Simic1

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 921-941, 2023, DOI:10.32604/phyton.2023.024995

    Abstract Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed to… More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analysis of Salt-Stress-Responsive Genes in Rice Roots

    Rui Song1, Yan Huang2, Xin Ji3, Yunfei Wei3, Qiuyuan Liu3, Shumei Li3, Juan Liu3,*, Pengfei Dong1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 237-250, 2023, DOI:10.32604/phyton.2022.023081

    Abstract Soil salinity greatly impairs plant growth and crop productivity. Rice (Oryza sativa L.) is a salt-sensitive crop. To better understand the molecular mechanisms of salt tolerance in roots, the BGISEQ-500 sequencing platform was employed to elucidate transcriptome changes in rice roots after 0, 3, 24, and 72 h of salt stress. The results showed that root K+ content decreased and Na+ content increased rapidly after the initial stage of salt stress, but that fresh and dry weight in root did not significantly reduce. Compared to the control (no salt stress), 1,292, 453, and 486 differentially expressed genes (DEGs) were upregulated,… More >

  • Open Access

    ARTICLE

    T-Shaped Transmission Line Fault Location Based on Phase-Angle Jump Checking

    Jia’an Xie1,*, Yurong Wang2, Guobin Jin3, Mucheng Wu1

    Energy Engineering, Vol.119, No.5, pp. 1797-1809, 2022, DOI:10.32604/ee.2022.020344

    Abstract In order to effectively solve the dead-zone and low-precision of T-shaped transmission line fault location, a new T-shaped transmission line fault location algorithm based on phase-angle jump checking is proposed in this paper. Firstly, the 3-terminal synchronous fundamental positive sequence voltage and current phasors are extracted and substituted into the fault branch distance function to realize the selection of fault branch when the fault occurs; Secondly, use the condition of the fundamental positive sequence voltage phasor at the fault point is equal to calculate all roots (including real root and virtual roots); Finally, the phase-angle jump check function is used… More >

  • Open Access

    ARTICLE

    Participation of Auxin Transport in the Early Response of the Arabidopsis Root System to Inoculation with Azospirillum brasilense

    Elizabeth Carrillo-Flores1, Jonanci Arreola-Rivera1, Denní Mariana Pazos-Solís2, Moisés Bocanegra-Mondragón2, Grisel Fierro-Romero3, Ma. Elena Mellado-Rojas1, Elda Beltrán-Peña1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2383-2401, 2022, DOI:10.32604/phyton.2022.021507

    Abstract The potential of Plant Growth Promoting Rhizobacteria (PGPR) has been demonstrated in the case of plant inoculation with bacteria of the genus Azospirillum which improves yield. A. brasilense produces a wide variety of molecules, including the natural auxin indole-3-acetic acid (IAA), as well as other phytoregulators. However, several studies have suggested that auxin induces changes in plant development during their interaction with the bacteria. The effects of A. brasilense Sp245 on the development of Arabidopsis thaliana root were investigated to help explain the molecular basis of the interaction. The results obtained showed a decrease in primary root length from the… More >

  • Open Access

    ARTICLE

    Exogenous melatonin alleviated growth inhibition and oxidative stress induced by drought stress in apple rootstock

    MEIGE WANG, JUAN GONG, CHUNHUI SONG, ZHENGYANG WANG, SHANGWEI SONG, JIAN JIAO, MIAOMIAO WANG, XIANBO ZHANG*, TUANHUI BAI*

    BIOCELL, Vol.46, No.7, pp. 1763-1770, 2022, DOI:10.32604/biocell.2022.018934

    Abstract

    Drought stress is one of the major environmental obstacles that limit the production and development of apples (Malus domestica Borkh.). The role of melatonin is well known in the protection of plants under environmental stresses. In this study, we investigated the effect of melatonin on apple rootstock M. hupehensis Rehd under drought stress. The results showed that drought inhibited the growth of M. hupehensis and dramatically reduced root surface area, root volume, the number of tips and forks, and root diameter. Drought-induced growth inhibition was significantly decreased by adding melatonin. Net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomatal conductance… More >

  • Open Access

    ARTICLE

    Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid, Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

    Longhua Zhou1,2,#, Ting He1,2,#, Jing Li1,2,3, Guimei Guo1,2, Yingbo Li1,2, Hongwei Xu1,2, Runhong Gao1,2, Linli Huang1,2, Yifei Wang1,2, Ruiju Lu1,2, Zhiwei Chen1,2,*, Chenghong Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

    Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

  • Open Access

    ARTICLE

    Constructing Collective Signature Schemes Using Problem of Finding Roots Modulo

    Tuan Nguyen Kim1,*, Duy Ho Ngoc2, Nikolay A. Moldovyan3

    CMC-Computers, Materials & Continua, Vol.72, No.1, pp. 1105-1122, 2022, DOI:10.32604/cmc.2022.025653

    Abstract Digital signature schemes are often built based on the difficulty of the discrete logarithm problems, of the problem of factor analysis, of the problem of finding the roots modulo of large primes or a combination of the difficult problems mentioned above. In this paper, we use the new difficult problem, which is to find the root in the finite ground field to build representative collective signature schemes, but the chosen modulo p has a special structure distinct , where is an even number and are prime numbers of equal magnitude, about . The characteristics of the proposed scheme are: i)… More >

  • Open Access

    ARTICLE

    POM analysis and computational interactions of 8-hydroxydiospyrin inside active site of protein tyrosine phosphatase 1B

    SAUD BAWAZER1, ASGHAR KHAN2, ABDUR RAUF3,*, TAIBI BEN HADDA4, YAHYA S. AL-AWTHAN5,6, OMAR BAHATTAB5, UMER RASHID7, INAMULLAH KHAN8, MUHAMMAD ASIF NAWAZ9, MD SAHAB UDDIN10,11, OLATUNDE AHMED12, MOHAMMAD ALI SHARIATI13

    BIOCELL, Vol.45, No.3, pp. 751-759, 2021, DOI:10.32604/biocell.2021.014004

    Abstract Protein tyrosine phosphatase 1B (PTP1B) inhibition is considered as a potential therapeutic for the treatment of cancer, type 2 diabetes, and obesity. In our present work, we investigated the anti-diabetic potential of 8-hydroxydiospyrin (8-HDN) from D. lotus against the PTP1B enzyme. It showed significant inhibitory activity of PTP1B with an IC50 value of 18.37 ± 0.02 μM. A detailed molecular docking study was carried out to analyze the binding orientation, binding energy, and mechanism of inhibition. A comparative investigation of 8-HDN in the catalytic, as well as the allosteric site of PTP1B, was performed. Binding energy data showed that compound… More >

  • Open Access

    ARTICLE

    Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds

    Anna Stepanova1,*, Maria Malunova1, Svetlana Salamaikina1, Renat Selimov2, Aleksandra Solov’eva1

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

    Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More >

  • Open Access

    ARTICLE

    Effects of Exogenous Manganese (Mn) on Mineral Elements, Polyamines and Antioxidants in Apple Rootstock Malus robusta Rehd.

    Dazhuang Qi1,2,4,#, Meixia Liang1,2,4,#, Fudong Jiang3, Jianzhao Li1,2,4, Xuqiang Qiao1,2,4,*, Hongxia Zhang1,2,4,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 943-955, 2020, DOI:10.32604/phyton.2020.013801

    Abstract Manganese (Mn) is one of the essential microelements in all organisms. However, high level of Mn is deleterious to plants. In this study, the effects of exogenous manganese application on mineral element, polyamine (PA) and antioxidant accumulation, as well as polyamine metabolic and antioxidant enzyme activities, were investigated in Malus robusta Rehd., a widely grown apple rootstock. High level of Mn treatments decreased endogenous Mg, Na, K and Ca contents, but increased Zn content, in a Mn-concentration-dependent manner. Polyamine metabolic assays revealed that, except the content of perchloric acid insoluble bound (PIS-bound) spermine, which increased significantly, the contents of putrescine… More >

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