Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (5)
  • Open Access


    Heat exposure promotes apoptosis and pyroptosis in Sertoli cells


    BIOCELL, Vol.47, No.1, pp. 155-164, 2023, DOI:10.32604/biocell.2023.024657

    Abstract Heat stress is an important influence on the male reproductive organs. Therefore, the effects of heat stress on genes or pathways related to the reproductive system of male mice were experimentally explored in this paper to further determine the effects of heat stimulation on mammals. Herein, models of heat-exposed mouse testicular tissue and heat-excited cells were successfully established. Many scorched vesicles were found after heat excitation of testis supporting cells, testicular mesenchymal (TM4) cells. Western blot, in situ terminal deoxynucleotide transferase dUTP Nick end labeling (TUNEL) and transmission electron microscopy showed that membrane rupture, mitochondrial damage and autophagic vesicles occurred… More >

  • Open Access


    Cloning and Drought Resistance Analysis of Soybean GmHsps_p23-like Gene

    Yuzhe Zhang1,#, Guanglong Li1,#, Shaowang Hu1, Jinfeng Liu1, Yushi Jiang1, Siyan Liu2,*, Shuyan Guan2,*, Jing Qu2, Dan Yao1, Andi Shi1, Yixuan Liu1

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1183-1198, 2022, DOI:10.32604/phyton.2022.018853

    Abstract Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance physiological and… More >

  • Open Access


    Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

    Muslum S. Inal1,2, Dilek Unal1,*, Bengu Turkyilmaz Unal3,*, Munir Ozturk4

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 583-598, 2022, DOI:10.32604/phyton.2022.018223

    Abstract Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis… More >

  • Open Access


    Technical Note : Prolonged exposure of human embryonic stem cells to heat shock induces necrotic cell death

    B.C. Heng*, K.J. Vinoth*, K. Lu*, X. Deng*, Z. Ge*, B.H. Bay**, T. Cao*

    BIOCELL, Vol.31, No.3, pp. 405-410, 2007, DOI:10.32604/biocell.2007.31.405

    Abstract We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane.… More >

  • Open Access


    Hyaluronic acid inhibited the upregulation of heat shock protein 70 in human chondrocytes from osteoarthritis and Kashin-Beck disease

    Zongqiang GAO1, Xiong GUO2, Junchang CHEN1, Chen DUAN2

    BIOCELL, Vol.43, No.2, pp. 99-102, 2019, DOI:10.32604/biocell.2019.06520

    Abstract This study aimed to investigate the effect of hyaluronic acid (HA) on the expression of heat shock protein 70 (HSP70) in chondrocytes isolated from patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The chondrocytes were collected from OA and KBD patients, and chondrocytes isolated from patients of accident injuries were used as the control. The chondrocytes were treated with HA at different doses. HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis. Compared with control, both mRNA and protein levels of HSP70 were higher in chondrocytes from KBD and OA. However,… More >

Displaying 1-10 on page 1 of 5. Per Page