Lili Zhao^*, Yao Zhang^*, Jiaoxia Liu^*, Wei Yin^†, Dan Jin^‡, Dandan Wang^*, Wei Zhang^*

Oncology Research, Vol.27, No.9, pp. 1015-1023, 2019, DOI:10.3727/096504018X15247341491655

Abstract MicroRNAs (miRNAs) are short endogenous noncoding RNAs that frequently play vital roles in many cancer types. Herein we demonstrated that miR-185 was remarkably downregulated in NSCLC tissues compared with adjacent normal tissues. A lower level of miR-185 was associated with lymph node metastasis. Functional assays showed that upregulation of miR-185 inhibited the proliferation, colony formation, and invasion capacities of NSCLC cells in vitro. Furthermore, we found that miR-185 suppressed the epithelial–mesenchymal transition (EMT) process. Bioinformatics analysis and luciferase reporter gene assays revealed that Kruppellike factor 7 (KLF7) was the target of miR-185. Overexpression of miR-185 reduced the expression of KLF7… More >

YANGUO PENG^1,#, WEI LI^2,#, SHUIYU CHEN¹, WANGCHUN WU¹, ZHANFANG SHANG^1,*

BIOCELL, Vol.45, No.1, pp. 57-64, 2021, DOI:10.32604/biocell.2021.011756

Abstract Peripheral nerve injury is a common neurodegenerative disease, which causes disability and a huge economic burden for patients. MicroRNAs (miRNAs) have been acknowledged as major regulators and therapeutic targets of neurological disease. Thus, the functional studies of miRNAs in neurological disease will contribute to discover new therapeutic targets for peripheral nerve injury. Sprague Dawley rats treated sciatic nerve surgical injury were regarded as peripheral nerve injury model in vivo. The expression of miR-494-3p and Kruppel like factor7 (KLF7) were measured by Real-time quantitative polymerase chain reaction (RT-qPCR) assay. In addition, western blot analysis was conducted to measure the protein levels… More >