Rabindranath Bera^1,#, Yotaro Ochi^2,3, Ying-Jung Huang¹, Ming-Chung Kuo^1,4, Kenichi Yoshida⁵, Seishi Ogawa², Lee-Yung Shih^1,4,*

Oncology Research, Vol.34, No.1, 2026, DOI:10.32604/or.2025.070259 - 30 December 2025

Abstract Background: Breakpoint Cluster Region-Abelson (BCR::ABL1) fusion protein is essential in the pathogenesis of chronic myeloid leukemia (CML); however, the chronic-to-blast phase transformation remains elusive. We identified novel kinesin light chain 2 (KLC2) mutations in CML-myeloid blast phase patients. We aimed to examine the functional role of KLC2 mutations in leukemogenesis. Methods: To evaluate the biological role of KLC2 mutants (MT) in CML cells, we expressed KLC2-MT in different human CML cell lines harboring BCR::ABL1 and performed immunoblot, immunofluorescence, cell proliferation, differentiation, and apoptosis; Tyrosine kinase inhibitor (TKI)-drug activities; and clonogenic assays for in vitro functional analyses. We co-expressed KLC2-MT… More >

Xiangmei Ye^1,2,3, Baoyi Yang⁴, Xin Zhang⁵, Luyuan Yang¹, Likun Zhang⁵, Qin Ren¹, Xiaobing Li¹, Leiguang Feng¹, Lanlan Wei^3,6,7,*, Peng Song¹, Yuqing Ye⁸, Xin Lian⁹, Yujuan Gao⁹, Haidi Tang¹, Zhiyu Liu¹

Oncology Research, Vol.34, No.1, 2026, DOI:10.32604/or.2025.068814 - 30 December 2025

Abstract Objectives: Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia and Philadelphia-like B-cell acute lymphoblastic leukemia (Ph+/Ph-like ALL) constitute the majority of relapsed/refractory B-ALL (R/R B-ALL) cases, highlighting an urgent need to discover new therapeutic targets. This study aims to elucidate the mechanisms underlying poor prognosis in Ph+/Ph-like ALL through transcriptome sequencing and functional cytological assays, with the goal of informing new clinical treatment strategies. Results: Transcriptomic analysis of Ph+/Ph-like ALL patients revealed that low expression of P2X Purinoceptor 1 (P2RX1) was associated with unfavorable outcomes. Specifically, patients with poor prognosis and low P2RX1 expression exhibited downregulation of… More >

Osama M. Alshehri¹, Ahmad Shaf^2,*, Muhammad Irfan^3,*, Mohammed M. Jalal⁴, Malik A. Altayar⁴, Mohammed H. Abu-Alghayth⁵, Humood Al Shmrany⁶, Tariq Ali⁷, Toufique A. Soomro⁸, Ali G. Alkhathami⁹

CMES-Computer Modeling in Engineering & Sciences, Vol.144, No.1, pp. 1165-1196, 2025, DOI:10.32604/cmes.2025.067150 - 31 July 2025

Abstract Background: Accurate classification of normal blood cells is a critical foundation for automated hematological analysis, including the detection of pathological conditions like leukemia. While convolutional neural networks (CNNs) excel in local feature extraction, their ability to capture global contextual relationships in complex cellular morphologies is limited. This study introduces a hybrid CNN-Transformer framework to enhance normal blood cell classification, laying the groundwork for future leukemia diagnostics. Methods: The proposed architecture integrates pre-trained CNNs (ResNet50, EfficientNetB3, InceptionV3, CustomCNN) with Vision Transformer (ViT) layers to combine local and global feature modeling. Four hybrid models were evaluated on… More >

MELISSA SáNCHEZ-RODRíGUEZ^1,2, ROBERTO LAZZARINI-LECHUGA³, VERóNICA SOUZA-ARROYO^1,4, LETICIA BUCIO-ORTIZ^1,4, ROXANA U. MIRANDA-LABRA^1,4, MONSERRAT GERARDO-RAMíREZ¹, ARACELI PáEZ-ARENAS⁵, MOISES VERGARA-MENDOZA⁶, MARíA CONCEPCIóN GUTIéRREZ-RUIZ^1,4, ALEJANDRO ESCOBEDO-CALVARIO^1,2,*, LUIS E. GOMEZ-QUIROZ^1,4,*

Oncology Research, Vol.33, No.8, pp. 2075-2084, 2025, DOI:10.32604/or.2025.064899 - 18 July 2025

Abstract Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype. Results: We found a profound impact on More >

BHAVYADHARSHINI ARUN^1,#, PRARTHANA GOPINATH^1,2,#, ANUP JHA^1,3, NISHTHA TRIPATHI¹, SYED G DASTAGER^4,*, SYED K HASAN^1,3,*

Oncology Research, Vol.33, No.8, pp. 2161-2174, 2025, DOI:10.32604/or.2025.062951 - 18 July 2025

Abstract Background: Acute Myeloid Leukemia (AML) is a highly aggressive clonal hematological malignancy with limited treatment options. This study aimed to evaluate the therapeutic potential of nigericin, a polyether ionophore derived from Streptomyces DASNCL-29, as a mitochondrial-targeted agent for AML treatment. Methods: Nigericin was isolated from Streptomyces DASNCL-29 and characterized via chromatography and NMR. Its cytotoxicity was tested in MOLM13 (sensitive and venetoclax-resistant) and HL60 (sensitive and cytarabine-resistant) cells using the MTT assay. Mitochondrial dysfunction was assessed by measuring reactive oxygen species (ROS), mitochondrial membrane potential (Δψm), and mitochondrial mass. Apoptosis was evaluated with Annexin V/PI assays… More > Graphic Abstract

Ali Raza^1,2,*, Feliz Minhós^2,3,*, Umar Shafique⁴, Muhammad Mohsin⁵

CMES-Computer Modeling in Engineering & Sciences, Vol.143, No.3, pp. 3411-3431, 2025, DOI:10.32604/cmes.2025.060855 - 30 June 2025

Abstract In 2022, Leukemia is the 13th most common diagnosis of cancer globally as per the source of the International Agency for Research on Cancer (IARC). Leukemia is still a threat and challenge for all regions because of 46.6% infection in Asia, and 22.1% and 14.7% infection rates in Europe and North America, respectively. To study the dynamics of Leukemia, the population of cells has been divided into three subpopulations of cells susceptible cells, infected cells, and immune cells. To investigate the memory effects and uncertainty in disease progression, leukemia modeling is developed using stochastic fractional… More >

Natalia Lehman, Agnieszka Bojarska-Junak, Michał Zarobkiewicz^*

BIOCELL, Vol.49, No.6, pp. 1085-1099, 2025, DOI:10.32604/biocell.2025.063960 - 24 June 2025

Abstract Introduction: Butyrophilins (BTNs) belong to the immunoglobulin superfamily; they play crucial roles in immune regulation, especially in γδ T cell activation. While their expression has been studied in solid tumours, their involvement in hematologic malignancies remains poorly understood. Objectives: We hypothesised that BTNs are dysregulated in chronic lymphocytic leukaemia (CLL), contributing to γδ T cell dysfunction and potentially influencing disease progression. Methods: In this study, we analyzed publicly available microarray and RNA-seq datasets to investigate the expression patterns of BTN genes in CLL. Results: Our findings reveal significant dysregulation of BTN gene expression in CLL, with BTN2A1, BTN3A1, BTN3A2,… More >

Reza Goodarzi¹, Ali Jalali^1,*, Omid Hashemi Pour Tafreshi¹, Jalil Mazloum¹, Peyman Beygi²

CMC-Computers, Materials & Continua, Vol.84, No.1, pp. 653-679, 2025, DOI:10.32604/cmc.2025.062803 - 09 June 2025

Abstract Acute lymphoblastic leukemia (ALL) is characterized by overgrowth of immature lymphoid cells in the bone marrow at the expense of normal hematopoiesis. One of the most prioritized tasks is the early and correct diagnosis of this malignancy; however, manual observation of the blood smear is very time-consuming and requires labor and expertise. Transfer learning in deep neural networks is of growing importance to intricate medical tasks such as medical imaging. Our work proposes an application of a novel ensemble architecture that puts together Vision Transformer and EfficientNetV2. This approach fuses deep and spatial features to… More >

GIOVANA GOMES CHAGAS¹, RUAN PIMENTA², NAYARA IZABEL VIANA^1,*

Oncology Research, Vol.33, No.6, pp. 1283-1288, 2025, DOI:10.32604/or.2025.055100 - 29 May 2025

Abstract The monoclonal antibodies consist of an innovative form of immunotherapy, capable of defeating several diseases, such as cancer. It is an emergent and important theme, that advances evaluation, challenges, and future perspectives with high relevance to identify gaps in recent studies and to consolidate this general theme in only one research. Its action in Chronic and Acute Lymphoid Leukemia has been evaluated in several clinical trials, which were selected between 2022 and 2023, in order to understand better the monoclonal antibodies that were most studied. The biopharmaceutical compounds Ibrutinib, Obinutuzumab, Rituximab, Venetoclax, and Inotuzumab Ozogamicin… More >

LUIS MANUEL GONZáLEZ-RODRíGUEZ¹, LUIS MIGUEL JUáREZ-SALCEDO^1,*, JAVIER LOSCERTALES¹, EVA ARRANZ¹, JIMENA CANNATA-ORTIZ¹, JAVIER ORTIZ¹, MARIA JOSé LóPEZ DE LA OSA¹, ADRIáN ALEGRE¹, SAMIR DALIA^2,*

Oncology Research, Vol.33, No.3, pp. 505-517, 2025, DOI:10.32604/or.2025.058175 - 28 February 2025

Abstract T-prolymphocytic leukemia is a rare and aggressive hematological malignancy characterized by the clonal proliferation of mature lymphoid T-cells. The pathogenesis of T-PLL is closely linked to specific chromosomal abnormalities, primarily involving the proto-oncogene T-cell leukemia/lymphoma 1 gene family. Recent advancements in molecular profiling have identified additional genomic aberrations, including those affecting the Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway. This case report presents a patient with T-prolymphocytic leukemia whose cytogenetic and molecular analysis revealed a t(X;14)(q28;q11.2) translocation and a STAT5B mutation. Here, we aim to review the genetic and molecular underpinnings of T-prolymphocytic More >