FUAD GANDHI TORIZAL^1,2,*, FRANSISCUS FIANO ANTHONY KERANS³, ANNISA KHUMAIRA¹

BIOCELL, Vol.46, No.8, pp. 1885-1891, 2022, DOI:10.32604/biocell.2022.019591

Abstract The potential of mesenchymal stem cells (MSCs) in regenerative medicine has been largely known due to their capability to induce tissue regeneration in vivo with minimum inflammation during implantation. This adult stem cell type exhibit unique features of tissue repair mechanism and immune modulation mediated by their secreted factors, called secretome. Recently, the utilization of secretome as a therapeutic agent provided new insight into cell-free therapy. Nevertheless, a sufficient amount of secretome is necessary to realize their applications for translational medicine which required a proper biomanufacturing process. Several factors related to their production need to be considered to produce a… More >

MASSIMO CONESE^1,*, AURELIO PORTINCASA²

BIOCELL, Vol.46, No.8, pp. 1815-1826, 2022, DOI:10.32604/biocell.2022.019448

Abstract The treatment of nonhealing and chronic cutaneous wounds still needs a clinical advancement to be effective. Both mesenchymal stem cells (MSCs), obtained from different sources, and their secretome derived thereof (especially exosomes) can activate signaling pathways related to promotion of cell migration, vascularization, collagen deposition, and inflammatory response demonstrating prohealing, angiogenetic and anti-scarring capacities. On the other hand, biodegradable biomimetic scaffolds can facilitate endogenous cell attachment and proliferation as well as extracellular matrix production. In this Review, we revise the complex composites made by biomimetic scaffolds, mainly hydrogels, and MSC-derived exosomes constructed for cutaneous wound healing. Studies demonstrate that there… More >

PANKE CHEN^*, SHUAI MA

BIOCELL, Vol.46, No.8, pp. 1903-1909, 2022, DOI:10.32604/biocell.2022.018651

Abstract This work aimed to investigate the effects of calcitonin gene-related peptide (CGRP)-modified mesenchymal stem cells (MSCs) on vascular stenosis in carotid balloon-injured rats. The CGRP gene labeled with EGFP was transfected into bone marrow MSCs, and CGRP protein expression in MSCs was confirmed by immunofluorescence assays. A rat carotid balloon injury model was established using a surgical method. CGRP-modified MSCs were orthotopically transplanted into the injured area of the rats. At 28 days after cell transplantation, EGFP and CD31 expression was detected by immunofluorescence staining. Hematoxylin-eosin (H&E) staining was used to detect the intima/media area of the injured carotid artery… More >

CHANYUAN JIN^1,4,#, ZIYAO ZHUANG^2,4,#, LINGFEI JIA^3,4,*, YUNFEI ZHENG^2,4,*

BIOCELL, Vol.46, No.7, pp. 1717-1724, 2022, DOI:10.32604/biocell.2022.018706

Abstract Osteoporosis is a frequently occurring bone remodeling disorder worldwide with one characteristic being decreasing bone mineral density and a predisposition to bone fracture, which diminishes patients’ quality of life. Several studies showed that imbalance between the osteogenesis and adipogenesis of bone marrow mesenchymal stem cells (BMSCs) took part in the development of osteoporosis. In previous study, we found MIR22HG regulated the osteogenesis of human BMSCs positively. In this study, we found that MIR22HG was decreased during the adipogenesis of human BMSCs and exerted negative effects on adipogenesis with the involvement of Wnt/β-catenin signaling pathway both in vitro and in vivo.… More >

GIORDANO WOSGRAU CALLONI^1,*, MARCO AUGUSTO STIMAMIGLIO^2,*

BIOCELL, Vol.46, No.6, pp. 1375-1381, 2022, DOI:10.32604/biocell.2022.019681

Abstract Mesenchymal stem cells (MSCs) and their byproducts have been widely validated as potential therapeutic products for regenerative medicine. The therapeutic effects result mainly from the paracrine activity of MSCs, which consists of the secretion of bioactive molecules, whether dispersed in medium conditioned by cell culture or encapsulated in extracellular vesicles. The composition of the MSC secretome, which represents the set of these secreted cellular products, is crucial for the performance of the desired therapeutic functions. Different cell culture strategies have been employed to adjust the secretome composition of MSCs to obtain the best therapeutic responses for different clinical contexts. However,… More >

MIN TANG^1,#, XUELING HE^1,2,#, XINGHONG YAO¹, JIRUI WEN¹, MINGYUE BAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.6, pp. 1465-1472, 2022, DOI:10.32604/biocell.2022.018967

Abstract The present study was designed to investigate the role of estrogen receptor α (ERα) in biaxial tensile strain (BTS) regulated osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs). rBMSCs were derived from rats and overexpressed ERα. The rBMSCs were subjected to BTS at 1 Hz with a strain of 2% for 4 h per day, 3 days, with or without ERα inhibitor ICI 182,780 (ICI). Then, bone mineralization was performed by Alizarin Red Staining. The markers of osteogenic differentiation and downstream Wnt3a/β-catenin signaling were detected by western blotting. Results showed that BTS enhanced the osteogenic differentiation of rBMSCs,… More >

IGOR A. KHLUSOV^1,*, LARISA S. LITVINOVA^2,*, KRISTINA A. YUROVA², MARINA Y. KHLUSOVA³

BIOCELL, Vol.46, No.6, pp. 1365-1373, 2022, DOI:10.32604/biocell.2022.018917

Abstract Stem cell microterritories (niches), as a specialized part of the extracellular matrix (ECM), are considered an important target and tool for the development of new materials, medical implants, and devices. However, tissue bioengineering products that have stem cell niches of known size on the surface or in the bulk structure of artificial materials are practically unknown. This brief review attempts to draw attention to the problematic aspects of niches as specific parts of the ECM, such as their hierarchy and size for mesenchymal stromal/stem cells (MSCs). These parameters arise directly from numerous definitions of stem cell niches as specialized morphological… More >

W. BENTON SWANSON, YUJI MISHINA^*

BIOCELL, Vol.46, No.6, pp. 1445-1451, 2022, DOI:10.32604/biocell.2022.018781

Abstract Mesenchymal stem cells (MSCs) have long been regarded as critical components of regenerative medicine strategies, given their multipotency and persistence in a variety of tissues. Recently, the specific role of MSCs in mediating regenerative outcomes has been attributed (in part) to secreted factors from transplanted cells, namely extracellular vesicles. This viewpoint manuscript highlights the promise of cell-derived extracellular vesicles as agents of regeneration, enhanced by synergy with appropriate biomaterials platforms. Extracellular vesicles are a potentially interesting regenerative tool to enhance the synergy between MSCs and biomaterials. As a result, we believe these technologies will improve patient outcomes through efficient therapeutic… More >

YASHVI SHARMA¹, SHARDA RAY², SUJATA MOHANTY^1,*

BIOCELL, Vol.46, No.6, pp. 1435-1438, 2022, DOI:10.32604/biocell.2022.018612

Abstract Neurodegenerative disorders are a vicious woe to the public health and wellness. Uncertainty in their underlying causes, lack of effective biomarkers for their early detection, existence of only supportive therapy, and their ever rising incidence creates an unmatched need for targeted therapies. Mesenchymal Stem Cells (MSCs) have found to be promising candidates for regenerative and remedial therapy in neurodegenerative disorders, however several biological risks and practical issues impede in their translational utility. Deriving from MSCs are certain Extracellular Vesicles (EVs), which aid in the paracrine action of MSCs and have lately gained the scientific interest for their implacability in diverse… More >

BENSHUAI YOU¹, HUI QIAN^1,2,*

BIOCELL, Vol.46, No.6, pp. 1459-1463, 2022, DOI:10.32604/biocell.2022.018378

Abstract Exosomes, especially from mesenchymal stem cells, have attracted extensive attention in regeneration medicine. Mesenchymal stem cells derived exosomes (MSCs-exosomes) have shown anti-inflammatory, anti-oxidant, anti-apoptosis and tissue regeneration effects in a variety of tissue injury repair models. MSCs-exosomes hold many excellent properties such as low immunogenicity, biocompatibility, and targeting capability. With the in-depth study on the generation and function of exosomes, MSCs-exosomes are considered to be the bright stars in the field of regenerative medicine. However, there are still many obstacles to overcome in terms of exosomes isolation, clinical trials and safety evaluation. In this article, what we should focus on… More >