N.M. OCARINO*, A. BOZZI**, R.D.O. PEREIRA*, N.M. BREYNER**, V.L. SILVA*, P. CASTANHEIRA**, A.M. GOES**, R. SERAKIDES*

BIOCELL, Vol.32, No.2, pp. 175-183, 2008, DOI:10.32604/biocell.2008.32.175

Abstract 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them… More >

Danyang Yue¹, Yijuan Fan¹, Juan Lu¹, Mengxue Zhang¹, Jin Zhou¹, Yuying Bai¹, Jun Pan^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 144-144, 2019, DOI:10.32604/mcb.2019.07084

Abstract Mesenchymal Stem Cells (MSCs) are recruited to the musculoskeletal system following trauma [1] or chemicals stimulation [2]. The regulation of their differentiation into either bone or cartilage cells is a key question. The fluid shear stress (FSS) is of pivotal importance to the development, function and even the repair of all tissues in the musculoskeletal system [3]. We previously found that MSCs are sensitive enough to distinguish a slight change of FSS stimulation during their differentiation commitment to bone or cartilage cells, and the internal mechanisms. In detail, MSCs were exposed to laminar FSS linearly increased from 0 to 10… More >

Lili Dong¹, Yang Song^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 78-78, 2019, DOI:10.32604/mcb.2019.07130

Abstract It has been widely recognized that stem cells possess the potential of osteogenic differentiation, which greatly contribute to bone repair. Recently, accumulating evidences have indicated that mechanical cues are required for bone repair ^[1,2]. However, how local and recruited stem cells in the bone architecture receive the mechanical signals is poorly understood ^[3,4]. The purpose of this study is to demonstrate that macrophages potentially transduce the mechanical signals for stem cell osteogenic lineage. This demonstration has been carried out through a co-culture system to investigate the effect of macrophages which subjected to cyclic stretch on the osteogenic potential of bone… More >

S. Sun¹, S. Lipsky¹, M. Cho¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 213-213, 2006, DOI:10.32604/mcb.2006.003.213

Abstract This article has no abstract. More >

F. Li¹, X. Wang¹, X. Liao¹, C. Niyibizi¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 167-168, 2006, DOI:10.32604/mcb.2006.003.167

Abstract This article has no abstract. More >

I. A. Titushkin¹, M. Cho¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 151-151, 2006, DOI:10.32604/mcb.2006.003.151

Abstract This article has no abstract. More >

Entsar A. SAAD¹, Reda S. EL-DEMERDASH², Eman M. ABD EI-FATTAH¹

BIOCELL, Vol.43, No.2, pp. 73-80, 2019, DOI:10.32604/biocell.2019.07020

Abstract Cisplatin is a powerful anticancer drug but its nephrotoxic effects limit its clinical use. We aimed to evaluate the effect of mesenchymal stem cells (MSCs) injection or of captopril to counteract the cisplatin-induction of nephropathy. MSCs isolation, preparation and tracking, transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) expressions, kidney function tests, oxidative stress state, and histological examinations were done. Cisplatininduced nephropathy was indicated biochemically and confirmed histopathologically. MSCs treatment showed normal kidney architecture, and significantly decreased oxidative stress and TGF-β while increased IL-10 and improved kidney function tests. Rats treated with cisplatin + captopril showed noticeable kidney histopathological changes. Superior… More >

Nesrine EBRAHIM¹, Eman EHSAN², Eman Abd EL GHANY², Dina SABRY³, Ashraf SHAMAA⁴

BIOCELL, Vol.43, No.1, pp. 21-28, 2019, DOI:10.32604/biocell.2019.06111

Abstract The neonatal hypoxic–ischemic encephalopathy (HIE) is an important cause of neurological morbidity and mortality in neonates. Cell therapy is considered a promising method for treating severe neurological disorders such as this one. Stem cells have the capacity for self-renewal and differentiation into certain cell lineages. The present study was aimed to find out the most beneficial route of bone marrow-derived mesenchymal stem cells (BMSCs) administration for the attenuation of experimentally induced HIE in neonatal rats. Sixty neonatal rats were divided randomly into four groups. Group 1: control group. Group 2: rats were exposed to bilateral ligation of cephalic arteries. Group… More >

Ruikai Chen¹, Delphine Dean^1,*

Molecular & Cellular Biomechanics, Vol.14, No.3, pp. 153-169, 2017, DOI:10.3970/mcb.2017.014.153

Abstract Vascular smooth muscle cells (VSMCs) play an important role in regulating blood flow and pressure by contracting and relaxing in response to a variety of mechanical stimuli. A fully differentiated and functional VSMC should have both the ability to contract and relax in response to environmental stimuli. In addition, it should have the proper mechanical properties to sustain the mechanically active vascular environment. Stem cells can differentiate towards VSMC lineages and so could be used as a potential treatment for vascular repair. However, few studies have assessed the time it takes for stems cells to acquire similar mechanical property to… More >

Pranav S. Sapkal^1*, Abhaykumar M. Kuthe¹, Divya Ganapathy², Shantanu C. Mathankar³, Sudhanshu Kuthe⁴

Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 115-136, 2016, DOI:10.3970/mcb.2016.013.131

Abstract Biphasic calcium phosphate scaffolds with 20/80 HA/TCP ratio were fabricated using the 3D-Bioplotting system to heal critical size defects in rabbit tibia bone. Four different architectures were printed in a layer by layer fashion with lay down patterns viz. (a) 0°– 90°, (b) 0°– 45°– 90°– 135°, (c) 0°–108°– 216° and (d) 0°– 60°– 120°. After high-temperature sintering scaffolds were coated with collagen and were further characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity in Balb/c 3T3 cells at… More >