HAIYING YUE, CHUNHUI WANG, HUIJUN ZHU, QINGHUA DU, JIAN LI, XUE OU, XIANGDE LI, QIULU ZHONG, YITING XIE, DANJING LUO, YIHE LI, CHUNXIAO LIANG, XUEMEI XU, SONGNAN DU, WENQI LIU^*

BIOCELL, Vol.47, No.11, pp. 2445-2452, 2023, DOI:10.32604/biocell.2023.042512

Abstract Background: Nasopharyngeal carcinoma (NPC) is one of the most prevalent cancers in Southeast Asia. Sirtuin 2 (SIRT2) is a member of the NAD+-dependent deacetylase family and has been shown to play important roles in numerous biological processes. However, Its function in NPC remains uncertain. The primary aim of this study is to clarify the role of SIRT2 in NPC. Methods: In this research, we examined the effect of SIRT2 silencing on NPC cell proliferation and colony formation using vitro NPC cell lines. Co-immunoprecipitation and mass spectrometry was applied to identify SIRT2-interacting proteins in NPC cells. Results: In comparison to nasopharyngeal… More > Graphic Abstract

HUAQING MO^#, JINGYI SHEN^#, YUXIAO ZHONG, ZENAN CHEN, TONG WU, YANYU LV^*, YANYAN XIE, YANRONG HAO^*

Oncology Research, Vol.30, No.4, pp. 187-199, 2022, DOI:10.32604/or.2022.027534

Abstract Nasopharyngeal carcinoma (NPC) is the most prevalent human primary malignancy of the head and neck, and the presence of vasculogenic mimicry (VM) renders anti-angiogenic therapy ineffective and poorly prognostic. However, the underlying mechanisms are unclear. In the present study, we used miR-940 silencing and overexpression for in vitro NPC cell EdU staining, wound healing assay and 3D cell culture assay, and in vivo xenograft mouse model and VM formation to assess miR-940 function. We found that ectopic miR-940 expression reduced NPC cell proliferation, migration and VM, as well as tumorigenesis in vivo. By bioinformatic analysis, circMAN1A2 was identified as a… More >

YONGLI WANG^1,2,#, SHENHONG QU^2,#, YONG YANG¹, YING QIN², FEI LIU³, GUANGWU HUANG^1,*

BIOCELL, Vol.47, No.3, pp. 533-545, 2023, DOI:10.32604/biocell.2023.025280

Abstract Background: Kinesin family member 15 (KIF15) is a protein that regulates cell mitosis and plays an important role in the development and progression of several types of human cancers. However, the role of KIF15 in the development of nasopharyngeal cancer (NPC) is still unclear. Methods: The differential expression of KIF15 in NPC and para-carcinoma tissues was evaluated based on data collected from Gene Expression Omnibus (GEO) database and immunohistochemical analysis of clinical specimens collected from a patient cohort. Cell lines 5-8F and CNE-2Z were selected for the construction of KIF15‑knockdown cell models. CCK8 assay, flow cytometry, wound healing, Transwell and… More >

Bo Wu^1,#, Huacai Xiong^2,#, Yong Wang¹, Shankun Zhao¹, Hongsheng Lu¹, Wei Hu^3,*

Oncologie, Vol.24, No.3, pp. 553-563, 2022, DOI:10.32604/oncologie.2022.023025

Abstract Epidermal growth factor receptor (EGFR) is frequently overexpressed in multiple malignancies. Icotinib (IH), a new EGFR tyrosine kinase inhibitor, enhances radiosensitivity in various types of cancer, but its effect on nasopharyngeal carcinoma (NPC) remains unclear. Total 115 NPC tissue sections and 30 nasopharyngitis tissue sections were enrolled. The correlation of EGFR expression and clinicopathologic features of NPC was analyzed. Survival analysis was calculated by using univariate and multivariate regression analysis. A radioresistant NPC cell line, CNE-2R, was established with a gradient irradiation schedule. Cell viability, colony formation and EGFR expression of CNE-2/2R cells were examined. Significant higher expression of EGFR… More >

FEI LIU¹, JIAZHANG WEI², JIAO LAN¹, YONGLI WANG², JIANXIANG YE³, CHENG Su¹, MINGZHENG MO¹, FENGZHU TANG², BING LI², MIN LI², WEIMING DENG², LINSONG YE², Wenlin HUANG², JINGJIN WENG², WEI JIAO^1,*, SHENHONG QU^2,*

BIOCELL, Vol.46, No.12, pp. 2659-2669, 2022, DOI:10.32604/biocell.2022.022886

Abstract Our previous studies suggested a potential interaction between the POK erythroid myeloid ontogenic factor ZBTB7A and glucose transporter 1 (GLUT1) in nasopharyngeal carcinoma (NPC). This study was designed to confirm the interaction and further evaluate the precise mechanism by which ZBTB7A and GLUT1 regulate NPC development. The binding sites between ZBTB7A and the promoter of GLUT1 were predicted by bioinformatics. Gene expression was measured by quantitative real-time polymerase chain reaction (qPCR), western blotting, and immunohistochemistry. The activities of key glycolysis enzymes, including hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and lactate, were detected using specific enzyme-linked immunosorbent assay kits.… More >

Linglan Gu, Yi Shi, Weimin Xu, Yangyang Ji

Oncology Research, Vol.27, No.8, pp. 923-933, 2019, DOI:10.3727/096504019X15518706875814

Abstract In previous investigations, we reported that peroxisome proliferator-activated receptor / (PPAR / ) activation by GW501516 inhibits proliferation and promotes apoptosis in the undifferentiated C666-1 nasopharyngeal carcinoma (NPC) cells by modulating caspase-dependent apoptotic pathway. In the present study, the mechanism by which GW501516 induces apoptosis was explored from the perspective of microRNA (miRNA) expression. Among the assayed miRNAs that were involved in regulating the expression of antiapoptotic protein Bcl-2, miR-206 was increased significantly and specifically by GW501516 in C666-1 cells at both the in vitro level and at the in vivo xenograft samples. The induction on miR-206 expression caused by… More >

Zhenghua Fei^*1, Zhenxiang Deng^†1, Lingyang Zhou^‡, Kejie Li^*, Xiaofang Xia^*, Raoying Xie^*

Oncology Research, Vol.27, No.7, pp. 801-807, 2019, DOI:10.3727/096504018X15446984186056

Abstract Nasopharyngeal cancer (NPC) is a malignant epithelial carcinoma of the head and neck. Cancer therapy targeting programmed cell death protein-1 (PD-1) or programmed death ligand-1 (PD-L1) is revolutionary. However, the tumorigenic mechanism of PD-L1 is not yet clear in NPC. Here we demonstrated an oncogenic role of PD-L1 via activating PI3K/AKT in NPC cells. PD-L1 overexpression was frequently detected in NPC biopsies and cell lines by qRT-PCR. PD-L1 overexpression and knockdown demonstrated that PD-L1 promoted NPC cell invasion and metastasis in vitro and in vivo. Mechanistically, PD-L1 prominently activated the epithelial–mesenchymal transition (EMT) process in a PI3K/AKT-dependent manner. Taken together,… More >

Jiandong Guo, Huihua You, Dong Li

Oncology Research, Vol.27, No.5, pp. 601-611, 2019, DOI:10.3727/096504018X15399945637736

Abstract Baicalein, an active ingredient separated from Astragalus membranaceus, has shown its anticancer ability in various cancers. However, its effect on nasopharyngeal carcinoma has not been explored yet. The present study aimed to investigate the effect of baicalein on the growth, proliferation, apoptosis, and cell cycle of human nasopharyngeal carcinoma cells, as well as transplanted nude mouse xenograft. The results showed that baicalein inhibited the growth and proliferation of CNE1 and CNE2 cells in a time- and concentration-dependent manner. It also caused a significant increase in the number of cells in the G₀/G₁ phase and a decrease in the G₂/M phase,… More >

Piao Yongfeng^*†‡§1, Jiang Chuner^*¶#1, Wang Lei^*†‡§, Yan Fengqin^*†‡§, Ye Zhimin^*†‡§, Fu Zhenfu^*†‡§, Jiang Haitao^*,**††, Jiang Yangming^‡‡, Wang Fangzheng^*†‡§

Oncology Research, Vol.28, No.6, pp. 605-613, 2020, DOI:10.3727/096504020X16022401878096

Abstract The aim of this study was to explore the predictive role of pretreatment MRI-based radiomics on early response of neoadjuvant chemotherapy (NAC) in locoregionally advanced nasopharyngeal carcinoma (NPC) patients. Between January 2016 and December 2016, a total of 108 newly diagnosed NPC patients who were hospitalized in the Cancer Hospital of the University of Chinese Academy of Sciences were reviewed. All patients had complete data of enhanced MR of nasopharynx before treatment, and then received two to three cycles of TP-based NAC. After 2 cycles of NAC, enhanced MR of nasopharynx was conducted again. Compared with the enhanced MR images… More >

Yanfen Guo^1,2, Zhe Cui¹, Xiaojie Li^2,*, Jing Peng^1,2, Jinrong Hu², Zhipeng Yang³, Tao Wu², Imran Mumtaz⁴

Intelligent Automation & Soft Computing, Vol.31, No.3, pp. 1771-1782, 2022, DOI:10.32604/iasc.2022.019785

Abstract Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors of the head and neck, and its incidence is the highest all around the world. Intensive radiotherapy using computer-aided diagnosis is the best technique for the treatment of NPC. The key step of radiotherapy is the delineation of the target areas and organs at risk, that is, tumor images segmentation. We proposed the segmentation method of NPC image based on multi-scale cascaded fully convolutional network. It used cascaded network and multi-scale feature for a coarse-to-fine segmentation to improve the segmentation effect. In coarse segmentation, image blocks and data augmentation… More >