Mohamed reda Kachmar^1,2, Toufik Bouddine³, Abdelaziz Ed-dra^4,5, Safaâ Kachmar⁶, Hammou Anarghou^7,8, Sulaiman Mohammed Alnasser⁹, Fahad M. Alshabrmi¹⁰, Samiah Hamad Al-Mijalli^11,*, Emad M. Abdallah^12,*, Nidal Naceiri Mrabti¹³, Abdelmounaim Laabar¹⁴, Mourad Chikhaoui¹⁵, Galman Aziz^1,4, Lhoussain Hajji³, Said Chakir²

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 657-677, 2025, DOI:10.32604/phyton.2025.062289 - 31 March 2025

Abstract Juniperus oxycedrus (J. oxycedrus) is a traditional culinary spice and medicinal herb with a longstanding history of ethnopharmacological applications across diverse cultures. While prior research has explored the biological activities and phytochemical constituents of extracts derived from its leaves and seed cones, the present study systematically investigates their mineral and phenolic profiles alongside their multifunctional bioactive potential. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) analysis revealed a substantial abundance of essential macro- and microelements. Reversed-phase high-performance liquid chromatography (RP-HPLC) further identified high concentrations of phenolic acids (e.g., p-coumaric acid) and flavonoids (e.g., rutin and quercetin). The extracts exhibited More >

Mehmet Fidan¹, Behcet Inal^2,*, Mesut Sırrı³, Bülent Hallaç⁴, Ulutas Mehmet Sefa¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 781-791, 2025, DOI:10.32604/phyton.2025.062171 - 31 March 2025

Abstract The use of conventional herbal medicines is a rapidly expanding phenomenon in developed nations. For instance, 30%–50% of all drug use in China is attributed to traditional herbal preparations. Current study evaluated the antioxidant (DPPH, FRAP), total phenolic and flavonoid content, antibacterial activity, and DNA damage protective potential of aqueous and methanolic extracts obtained from the aerial parts and roots of Cymbocarpum wiedemannii Boiss., an endemic plant in Turkey. In antioxidant analyses, the methanolic extract of the aerial parts showed the highest %DPPH (73.38) and IC50 (3.46 mg/mL) values. The FRAP analysis revealed the highest iron-reducing… More > Graphic Abstract

Deen Bandhu Dimri¹, Manisha Nigam^1,*, Naresh Singh¹, Shilpa Semwal¹, Marcello Iriti^2,3,*, Abhay Prakash Mishra⁴

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 1007-1027, 2025, DOI:10.32604/phyton.2025.061270 - 31 March 2025

Abstract Cornus capitata, commonly known as the Himalayan strawberry, is a dogwood species from the family Cornaceae. Dogwood plants have been used extensively to practice conventional medicine due to their biological activities; however, the mechanisms behind these activities, particularly in C. capitata, remain largely unexplored. This study focuses on the preliminary phytochemical screening, antioxidant activity, anti-diabetic potential, and antimicrobial profiling of different extracts (i.e., leaves, bark, and fruit) from C. capitata to evaluate its potential pharmaceutical and medicinal applications. The in-vitro antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, Ferric-reducing antioxidant power (FRAP) assay, and Superoxide Anion Scavenging assay.… More > Graphic Abstract

Gohar Ayoub¹, Shafaqat Ali^1,2,*, Muhammad Arslan Ashraf ^3,4, Shoaib Ahmad⁵, Mohamed A. El-Sheikh⁶

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 929-951, 2025, DOI:10.32604/phyton.2025.059288 - 31 March 2025

Abstract Chromium (Cr), a persistent soil pollutant, has detrimental effects on plants and living things, and its contamination in soil increased as a result of human-induced activities. Pakistan suffers from a lack of fresh water supplies; hence most people use metal-containing water and wastewater to irrigate their crops. Exposure to Cr toxicity, the plant reduces their morphological and physiological growth which ultimately decreases crop productivity. The current study was designed to investigate the foliar application of hesperidin (HSP) at varying effluent rates (25, 50, 75, and 100 mg L⁻¹) on wheat growth under tannery wastewater irrigated soil.… More >

Longgang Wang^2,3,#, Xiangxun Zhuansun^2,3,#, Yao Li^2,3, Qili Yao^2,3, Qi Liu^2,3,*, Huijing Lin^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 441-454, 2025, DOI:10.32604/phyton.2025.061720 - 06 March 2025

Abstract Fructus Ligustri Lucidi (FLL) refers to the dried mature fruit of Ligustrum lucidum Ait., a species from the Oleaceae family, widely distributed across East Asia and India. This study aimed to optimize the extraction process for Fructus Ligustri Lucidi essential oil (FLLO) to develop an efficient and practical extraction method. Additionally, the chemical composition of FLLO was analyzed, and its antioxidant, antimicrobial, and cytotoxic activities were evaluated. FLLO was extracted using supercritical CO₂ extraction, and response surface methodology was applied to optimize the extraction parameters: pressure of 16 MPa, temperature of 40°C, and extraction time of 40… More > Graphic Abstract

Jalil Ahmad^1,*, Muhammad Munir^2,*, Nashi Alqahtani^2,3, Tahira Alyas⁴, Muhammad Ahmad⁵, Sadia Bashir⁶, Fasiha Qurashi⁷, Abdul Ghafoor⁸, Hassan Ali–Dinar²

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 281-302, 2025, DOI:10.32604/phyton.2025.061534 - 06 March 2025

Abstract A steady rise in the overall population is creating an overburden on crops due to their global demand. On the other hand, given the current climate change and population growth, agricultural practices established during the Green Revolution are no longer viable. Consequently, innovative practices are the prerequisite of the time struggle with the rising global food demand. The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise. Nanoparticles (NPs) typically enhance plant resilience to stressors by fortifying the physical barrier, optimizing photosynthesis, stimulating enzymatic activity for defense, elevating More >

Amine Batbat^1,2, Mohamed El Fadili³, Naoufal El Hachlafi^4,*, Amine Elbouzidi^5,*, Chaymae Hmimen⁶, Hiba Yahyaoui^2,7, Samir Jeddi⁶, Abdellatif Benbouazza², Kawtar Fikri-Benbrahim⁶, Mohamed Addi⁵, Samiah Hamad Al-Mijalli⁸, Khaoula Habbadi², Hassane Greche¹

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 481-501, 2025, DOI:10.32604/phyton.2025.059841 - 06 March 2025

Abstract This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil (EO), an endemic medicinal plant of Morocco used for its various properties. The chemical composition of the EO was characterized using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity against different agricultural phytopathogens was determined by disc diffusion and microatmosphere methods, as well as by the determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC), while the antioxidant activity was evaluated by DPPH and FRAP assays. To complement the experimental analyses, a molecular docking approach was used to predict and elucidate… More >

Samiah Hamad Al-Mijalli¹, Hanae Naceiri Mrabti², Amine Elbouzidi^3,*, Naglaa S. Ashmawy^4,5, Amine Batbat⁶, Emad M. Abdallah⁷, Wafa Laaboudi⁸, Mohammed Aladhadh⁹, Fahad M. Alshabrmi¹⁰, Sulaiman Mohammed Alnasser¹¹, Mohamed Addi³, Naoufal El Hachlafi¹²

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 209-227, 2025, DOI:10.32604/phyton.2025.060438 - 24 January 2025

Abstract Thymus serpyllum L., often known as wild thyme, has been used since ancient times due to its multifaceted culinary and medicinal attributes. It is usually utilized in folk medicine to manage different health issues. This work aimed to investigate the chemical composition and biological characteristics of T. serpyllum essential oil (EO), including its antimicrobial, antioxidant, and anti-inflammatory capabilities. Moreover, we have prompted an in-silico simulation to reveal the underlying mode of action of these properties. The chemical characterization of T. serpyllum (EO) by Gas Chromatography-Mass Spectrometry (GC-MS) indicated sabinene (17.33%), terpinen-4-ol (11.73%), phellandral (13.18%), and thymol (10.54%) as main… More >

Marwa Rezgui^1,#,*, Wided Ben Ammar¹, Muhammad Nazim^2,3,#, Walid Soufan⁴, Chiraz Chaffei Haouari¹

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 137-155, 2025, DOI:10.32604/phyton.2025.058851 - 24 January 2025

Abstract Nickel (Ni) toxicity significantly impairs plant growth, photosynthesis, and metabolism by inducing oxidative stress. This study evaluates the potential of exogenous Alpha-Ketoglutarate (AKG) in mitigating Ni-induced stress in Peganum harmala L. Seedlings were exposed to 0, 200, 500, and 750 μM NiCl₂, with or without AKG supplementation. Under 750 μM Ni stress, dry weight (DW) decreased by 33.7%, tissue water content (TWC) by 39.9%, and chlorophyll a and total chlorophyll levels were reduced by 17% and 15%, respectively. Ni exposure also significantly increased secondary metabolite production, with leaf anthocyanin content rising by 131%, and superoxide dismutase (SOD)… More >

Ragini Singh¹, Noushina Iqbal^1,*, Shahid Umar^1,*, Sayeed Ahmad²

Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3237-3271, 2024, DOI:10.32604/phyton.2024.057189 - 31 December 2024

Abstract Lignans are a prominent group of phenolic compounds ubiquitously present in the plant kingdom, playing a critical role in both plant physiology and human health. Structurally they are characterized by the dimerization of two phenylpropane units to attain diverse chemical configurations that contribute to their wide range of biological activities. In plants, lignans function primarily as defense molecules, protecting against pathogens, herbivores, and environmental stressors. These compounds also participate in plant growth regulation and lignification processes. From a nutritional and medicinal perspective, lignans are valued for their significant health benefits. They act as phytoestrogens,… More >