Kuntal Das¹, Syed Mohammed Basheeruddin Asdaq^2,*, M. Saifulla Khan^1,*, Sravani Singirikonda¹, Abdulhakeem S. Alamri^3,4, Walaa F. Alsanie^3,4, Majid Alhomrani^3,4, Sreeharsha Nagaraja^5,6, Katharigatta N. Venugopala^5,7

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2349-2365, 2022, DOI:10.32604/phyton.2022.022379

Abstract The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence of flavonoid components in… More >

J. David García-Ortíz¹, Rebeca González-Centeno¹, María Alejandra Torres-Tapia², J. A. Ascacio-Valdés¹, José Espinoza-Velázquez², Raúl Rodríguez-Herrera^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 929-941, 2022, DOI:10.32604/phyton.2022.018368

Abstract Because of the growing worldwide demand for maize grain, new alternatives have been sought for breeding of this cereal, e.g., development of polyembryonic varieties, which agronomic performance could positively impact the grain yield per unit area, and nutritional quality. The objectives of this study were to (1) determine the phytochemicals present in the embryo and endosperm of grain from maize families with high, low, and null polyembryony frequency, which were planted at different locations, and (2) state the relationship between these compounds and seed germination. The extracted phytochemicals from corn were identified by HPLC-MS. The results showed that the genotype… More >

Xiaojun Pan¹, Haiying Li², Xiaoyi Li¹, Li Xu¹, Yanbin Sun^1,*

CMC-Computers, Materials & Continua, Vol.71, No.2, pp. 2401-2417, 2022, DOI:10.32604/cmc.2022.021020

Abstract Supervisory control and data acquisition systems (SCADAs) play an important role in supervising and controlling industrial production with the help of programmable logic controllers (PLCs) in industrial control systems (ICSs). A PLC receives the control information or program from a SCADA to control the production equipment and feeds the production data back to the SCADA. Once a SCADA is controlled by an attacker, it may threaten the safety of industrial production. The lack of security protection, such as identity authentication and encryption for industrial control protocols, increases the potential security risks. In this paper, we propose a PLC protection system… More >

POOJA BHADRECHA¹, MADHU BALA^2,3, VIKAS KAUSHIK⁴, NASEEM A. GAUR⁵, SIMRANJEET SINGH⁴, JOGINDER SINGH^4,*, MANOJ KUMAR^6,*

BIOCELL, Vol.45, No.2, pp. 387-394, 2021, DOI:10.32604/biocell.2021.013824

Abstract Hippophae rhamnoides L. is a plant of immense ethnopharmacological importance and is a known source for various valuable biochemicals and nutraceuticals. The production of folate, a vitamin involved in several vital functions, in this plant is rather poorly understood. Herein, we investigate the hypothesis that rhizobial bacteria serve the plant in this essential vitamin’s biosynthesis. Bacterial strains of Bacillus, Azorhizobium, Frankia, Paenibacillus, Brevibacillus and Pseudomonas, were isolated from the rhizosphere of the plant. HPLC and LCMS were used to trace the production of intra and extra-cellular folate by representative rhizospheric bacterial strains in vitro. From the seventeen functionally characterized bacterial… More >

Anna Stepanova^1,*, Maria Malunova¹, Svetlana Salamaikina¹, Renat Selimov², Aleksandra Solov’eva¹

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More >

A. Bikoro Bi Athomo^1,2, S. P. Engozogho Anris^1,2, R. Safou Tchiama^2,3, F. Eyma⁴, C. Arnaudguilhem⁵, B. Charrier^1,*

Journal of Renewable Materials, Vol.9, No.1, pp. 35-48, 2021, DOI:10.32604/jrm.2021.013626

Abstract

A complementary approach using Liquid Chromatographic-Mass Spectrometric analysis was proposed to characterize phenolic compounds from the methanol-water extracts of K. ivorensis A. Chev. Two High Performance Liquid Chromatography (HPLC) complementary methods were used for the determination of phenolic compounds from the bark, sapwood and heartwood of K. ivorensis. Methods employed involved direct analysis after filtration at 0.20 µm, using a RP C₁₈ column and UV-VIS/ESI-FTMS detection. The methods used were different by their elution gradient and allowed analyzing the chemical composition of three parts of African mahogany extracts. In this study, 22 phenolic compounds and derivatives from K. ivorensis were… More >

Arnab Kumar De, Arijit Ghosh, Debabrata Dolui, Indraneel Saha, Malay Kumar Adak^*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 999-1017, 2020, DOI:10.32604/phyton.2020.010828

Abstract In the present experiment with ongoing concentration (0 µM, 100 µM, 250 µM, 500 µM and 1000 µM) of 2,4-D, the responses of Azolla pinnata R.Br. was evaluated based on cellular functions. Initially, plants were significantly tolerated up to 1000 µM of 2,4-D with its survival. This was accompanied by a steady decline of indole acetic acid (IAA) concentration in tissues with 78.8% over the control. Membrane bound H⁺ -ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator (KCl) and decreased within a range of 57.3 to 74.6% in response to inhibitor (Vanadate) application.… More >

Weizu Huang¹, Dong Wang^{1, *}, Zuodong Xiao¹, Qiang Yao², Danjie Du³

CMC-Computers, Materials & Continua, Vol.65, No.1, pp. 897-912, 2020, DOI:10.32604/cmc.2020.09728

Abstract The vibratory roller is a piece of vital construction machinery in the field of road construction. The unmanned vibratory roller efficiently utilizes the automated driving technology in the vehicle engineering field, which is innovative for the unmanned road construction. This paper develops and implements the autonomous construction system for the unmanned vibratory roller. Not only does the roller have the function of remote-controlled driving, but it also has the capability of autonomous road construction. The overall system design uses the Programmable Logic Controller (PLC) as the kernel controller. It establishes the communication network through multiple Input/Output (I/O) modules, Recommended Standard… More >

Joo-Chan Lee¹, Hyun-Pyo Choi¹, Jang-Hoon Kim¹, Jun-Won Kim¹, Da-Un Jung¹, Ji-Ho Shin¹, Jung-Taek Seo^{1, *}

CMC-Computers, Materials & Continua, Vol.65, No.1, pp. 53-67, 2020, DOI:10.32604/cmc.2020.011251

Abstract Cyberattacks on the Industrial Control System (ICS) have recently been increasing, made more intelligent by advancing technologies. As such, cybersecurity for such systems is attracting attention. As a core element of control devices, the Programmable Logic Controller (PLC) in an ICS carries out on-site control over the ICS. A cyberattack on the PLC will cause damages on the overall ICS, with Stuxnet and Duqu as the most representative cases. Thus, cybersecurity for PLCs is considered essential, and many researchers carry out a variety of analyses on the vulnerabilities of PLCs as part of preemptive efforts against attacks. In this study,… More >

Xiaojun Pan, Zhuoran Wang, Yanbin Sun^*

Journal of Cyber Security, Vol.2, No.2, pp. 69-83, 2020, DOI:10.32604/jcs.2020.010045

Abstract Programmable Logic Controllers (PLC), core of industrial control systems, is widely used in industrial control systems. The security of PLC is the key to the security of industrial control systems. Nowadays, a large number of industrial control systems are connected to the Internet which exposes the PLC equipment to the Internet, and thus raising security concerns. First of all, we introduce the basic principle of PLC in this paper. Then we analyze the PLC code security, firmware security, network security, virus vulnerability and Modbus communication protocol by reviewing the previous related work. Finally, we make a summary of the current… More >