MINGHONG WEI^1,#, CHENG XIAO^1,2,#, JIAN WU^1,3, LIHONG QIN^1,3, HONGLIANG LIU^1,3, YANG CAO^1,*, YUMIN ZHAO^1,3,*

BIOCELL, Vol.47, No.9, pp. 2115-2123, 2023, DOI:10.32604/biocell.2023.030364

Abstract Background: The regulatory genes related to lipid metabolism affect the intramuscular fat (IMF) content and improve meat quality traits. Mediator Complex Subunit 4 (MED4), a vitamin D receptor protein, affects vitamin D, livestock growth, carcass traits, and triglyceride deposition. However, the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown. Methods: This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle. The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain… More >

YUE GUAN^1,#, TINGTING ZHANG^2,#, JIANAN YU³, JIAWEI LIU⁴, WENYUAN LI⁵, YUJIA ZHENG⁶, JIALE WANG³, YUE LIU³, FENGGUO ZHAI^2,7,*

BIOCELL, Vol.47, No.7, pp. 1583-1594, 2023, DOI:10.32604/biocell.2023.028016

Abstract Background: A preliminary miRNA screening showed that expression levels of rno-miRNA-27a-3p were significantly increased in the serum and brain tissues of rats undergoing cerebral ischemia. In recent years, there is evidence of the protective capacity of the saponins extracted from panax ginseng and its primary active ingredient ginsenosideRg1oncerebral ischemic injury. Methods: Fetal rat neurons (FRNs) were cultured in glucose-and-serum-free medium and exposed to hypoxia to establish a cerebral ischemia model in vitro (oxygen and glucose deprivation model, OGD). Antioxidant indexes (CAT, SOD), inflammatory markers (MPO, TNF-α and IL-6), and the expression of apoptosis and proliferation associated proteins (NF kB-p65, Caspase… More >

ABDELAZIZ ADAM IDRISS ARBAB^1,3,#, CHUNQING YIN^4,#, XUBIN LU¹, YAN LIANG¹, ISMAIL MOHAMED ABDALLA¹, AMER ADAM IDRIS³, TIANLE XU^1,2, YONGJIANG MAO¹, ZHANGPING YANG^1,2,*

BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865

Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ (PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence analysis were conducted to evaluate the… More >

XIAOMEI HAN^#, SHUYING ZHANG^#, YIFU WANG, CHANGE QI, PENGNYU GUO, YALI XU, GUANGHUI LYU^*

BIOCELL, Vol.45, No.6, pp. 1509-1519, 2021, DOI:10.32604/biocell.2021.09506

Abstract Diabetes Mellitus is a systematic disease with complications in multi-organs, including decreased implant osseointegration and a high failure rate of dental transplants. Accumulating evidence indicates that the signaling pathway directly impacts the process of bone metabolism and inflammatory response implicated with dental implants in diabetic patients. This review summarizes the recent advance in signaling pathways regulate osseointegration and inflammatory response in dental transplantation, aiming to identify the potential therapeutic target to reduce the dental transplant failure in diabetes patients, with emphasis on the surface characteristics of the implant, inflammatory signaling, AMPK, PPARγ, WNT, ROS, and adiponectin signaling. More >

BIDYUT MALLICK^1,#, ASHISH RANJAN SHARMA^2,#, MANOJIT BHATTACHARYA³, SANG-SOO LEE^1,*, CHIRANJIB CHAKRABORTY^4,*

BIOCELL, Vol.45, No.4, pp. 953-961, 2021, DOI:10.32604/biocell.2021.015530

Abstract PPARγ is a peroxisome proliferator-activated receptor (PPAR) family protein and is a target for type 2 diabetes (T2D). In this paper, we have performed a molecular docking analysis between ligand molecules (CID9816265, CID11608015, CID20251380, CID20251343, CID20556263, CID624491, CID42609928, and CID86287562) and PPARγ to determine the ligand specificity. It also helps to understand the ligand-binding domain (LBD) activity of PPARγ during the binding of the ligand. Further, a molecular dynamics simulation study was performed to determine the ligand biding stability in the PPARγ LBD. Its ligand specificity informed us about the potentiality of selecting a partial agonist. The study also shows… More >

Kun Fan^1,2, Xiaofu Qiu², Yu Fu², Kangjian Lin, Huanhui Li², Guosheng Yang ^*,1,2

Molecular & Cellular Biomechanics, Vol.14, No.1, pp. 33-45, 2017, DOI:10.3970/mcb.2017.014.035

Abstract This study intends to investigate the role of amentoflavone(AF) in human clear-cell renal cell carcinoma (ccRCC) and to elucidate underlying molecular mechanisms. Materials and Methods: Human RCC cell lines Caki-1 and 786-O were used in this study. Cell proliferation, apoptosis, cell cycle distribution and invasion assays were conducted to analyze the effect of AF against ccRCC in vitro. Xenograft model and pulmonary metastasis animal model were established to evaluate the vivo therapeutic efficacy and against pulmonary metastasis ability of AF, respectively. Results: Our findings revealed that AF selectively suppressed tumor cell proliferation in a dose- and time-dependent manner. Treatment with… More >