Javed Rashid^1,2, Imran Khan¹, Irshad Ahmed Abbasi³, Muhammad Rizwan Saeed⁴, Mubbashar Saddique^5,*, Mohamed Abbas^6,7

CMC-Computers, Materials & Continua, Vol.76, No.3, pp. 3897-3920, 2023, DOI:10.32604/cmc.2023.040356

Abstract Many plant species have a startling degree of morphological similarity, making it difficult to split and categorize them reliably. Unknown plant species can be challenging to classify and segment using deep learning. While using deep learning architectures has helped improve classification accuracy, the resulting models often need to be more flexible and require a large dataset to train. For the sake of taxonomy, this research proposes a hybrid method for categorizing guava, potato, and java plum leaves. Two new approaches are used to form the hybrid model suggested here. The guava, potato, and java plum plant species have been successfully… More >

Liping Hong, Enming He, Wenhui Zhang, Mingqiong Zheng, Jingwen Wu, Wenhua Wang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2785-2805, 2023, DOI:10.32604/phyton.2023.030928

Abstract Potato (Solanum tuberosum L.) is susceptible to enzymatic browning after fresh processing, resulting in color change and potential alteration in the nutritional quality. In this study, a popular potato cultivar, Feiwuruita, was used to profile the metabolites involved in color and nutritional quality changes in fresh shreds stored at 0 and 4 h at 25°C (designated CK and CK4H, respectively). The shreds turned brown within 4 h of storage. In all, 723 metabolites consisting 12 classes of compounds were detected in the samples, largely lipids, phenolic acids, alkaloids, amino acids and derivatives, flavonoids, organic acids, nucleotides and derivatives. Of these,… More >

Tahira Kalsoom¹, Touqeer Ahmed¹, Muhammad Azam Khan¹, Mirza Hasanuzzaman^2,*, Mukhtar Ahmed^3,*, Stefaan P. O. Werbrouck⁴

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2429-2450, 2023, DOI:10.32604/phyton.2023.030846

Abstract Potato propagation through tubers and seed is an inefficient way of propagation thus we propose an alternative method in this study which is in vitro propagation. Plantlets were raised from True Potato Seeds (TPS) in test tubes containing 10 mL of Murashige Skoog (MS) medium. Subculturing was done after every 21 days and after four subsequent subculturing, when there was an adequate quantity of stock material, uninodal cuttings of about 3–4 mm in length were prepared from in vitro proliferated potato plants by excising leaves of 1–2 mm on each side of the node. Prepared uninodal cuttings were cultured on… More >

Yangyang Du¹, Yuanyuan Bao², Lu Zheng¹, Qinglong Tian¹, Xinyong Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2325-2348, 2023, DOI:10.32604/phyton.2023.027642

Abstract Inorganic phosphate (Pi) is one of the main nutrients necessary for plant growth. Phosphate transporters mediate the acquisition, transport and recycling of phosphate, which is essential for plant growth and development. Although PHT1 has been reported in many plants at home and abroad, it is rarely studied in potato. Therefore, it is of great significance to study the PHT1 family members in order to understand the molecular response mechanism of potato in low phosphate state. In this study, a total of 6 potato PHT1 genes were identified and isolated. It was found that after the expression of different members of… More >

Hafiza Arooj Razzaq¹, Siddra Ijaz^1,*, Imran Ul Haq², Faisal Saeed Awan¹

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2451-2460, 2023, DOI:10.32604/phyton.2023.030501

Abstract The imperative aspect of the CRISPR/Cas9 system is a short stretch of 20 nucleotides of gRNA that control the overall specificity. Due to the small size, the chance of its multiple occurrences in the genome increases; however, a few mismatches are tolerated by the Cas9 endonuclease activity. An accurate and careful in silico-based off-target prediction while target selection is preferred to address the issue. These predictions are based on a comprehensive set of selectable parameters. Therefore, we investigated the possible off-target prediction and their screening in StERF3 gene-edited potato plants while developing StERF3-loss-of-function mutants using CRISPR/Cas9 approach. The 201 off-targets… More >

Touseef Hussain^1,*, Abrar A. Khan¹, Heba I. Mohamed^2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 783-799, 2023, DOI:10.32604/phyton.2022.026045

Abstract Fusarium dry rot is considered to be the most critical soilborne and postharvest disease that damages potato tubers worldwide when they are stored for a long time. This study was performed to demonstrate the effect of crude extract, culture filtrate, and cell suspension obtained from the bacterium Bacillus subtilis HussainT-AMU, on the net house and field. From oil-contaminated soil, through the serial dilution method, biosurfactant bacteria were isolated on nutrient agar medium. To isolate and screen the prospective biosurfactant strain, various biosurfactant screening methods were used. Standard protocols were carried out for morphological, molecular, and chemical characterization. The disease incidences… More >

KHANDAKAR ABU MD MOSTAFIZAR RAHMAN^1,2, ABDUL SHUKOR JURAIMI^2,*, MD. REZWAN MOLLA³, MUHAMMAD ASYRAF MD HATTA⁴, ZULKEFLY BIN SULAIMAN⁵, SHAMIMA SULTANA⁶, AHMED GABER⁷, BENUKAR BISWAS⁸, AKBAR HOSSAIN^9,*

BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135

Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information was effective in differentiating… More >

Diego David Pinzón-Moreno^*, Eder Clidio Vicuña-Galindo, José Vulfrano González-Fernández, José Luis Soto-Gonzales, María Verónica Carranza-Oropeza^*

Journal of Renewable Materials, Vol.10, No.8, pp. 2179-2201, 2022, DOI:10.32604/jrm.2022.019793

Abstract Several hydrogels were synthesized by free-radical polymerization in an aqueous medium based on potato starch (PS), poly(vinyl alcohol) (PVA), and N,N′-Methylenebisacrylamide (MBAm), being possible to study these hydrogels as a function of the proportion of components incorporated. In this way, the products generated from the synthesis were characterized by swelling and deswelling kinetics, the first swelling being verified with Schott and statistical models, allowing to contrast the proximity between the experimental and theoretical behavior. Additionally, water retention in soil (R%), spectroscopy (FTIR), morphological (SEM), and thermal (TGA and DSC) analysis allowing to know the intrinsic characteristics of the material, increasing… More >

Chengyang Li^1,2,#, Lianjun Wang^1,#, Shasha Chai^1,#, Yinghua Xu^1,2, Chong Wang^1,2, Yi Liu^1,2, Jian Lei¹, Xiaojie Jin¹, Xianliang Cheng¹, Yuanyuan Yang¹, Xinsun Yang^1,*, Wenying Zhang^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1779-1793, 2022, DOI:10.32604/phyton.2022.020192

Abstract Fusarium wilt, a disease caused by Fusarium oxysporum f.sp batatas (Fob) is an important disease in sweet potato production. Using endophytic bacteria for biological control of sweet potato diseases is one of the important ways. A Bacillus subtilis with antagonistic effect on Fusarium wilt of sweet potato was isolated from soil by confrontation culture. According to the biological characteristics, 16S rDNA sequence analysis, and physiological and biochemical analysis, the Bacillus subtilis HAAS01 was named. A pot experiment was conducted for the biological control experiment of strain HAAS01, and the endogenous hormone content, antioxidant enzyme activity, soluble protein content, and related… More >

YASSER S. MOSTAFA^1,*, SAAD A. ALAMRI^1,2, SULIMAN A. ALRUMMAN¹, TAREK H. TAHA³, MOHAMED HASHEM^1,4, MAHMOUD MOUSTAFA^1,5, LAMIAA I. FAHMY⁶

BIOCELL, Vol.45, No.6, pp. 1661-1672, 2021, DOI:10.32604/biocell.2021.016193

Abstract The study was sought to enhance the synthesis of thermal stable β-cyclodextrin glycosyltransferase (β-CGTase) using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production of β-cyclodextrin (β-CD) from raw potato starch. Thermophilic bacteria producing β-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA gene. Alginate-encapsulated cultures exhibited twice-fold of β-CGTase production more than free cells. Scanning electron microscopy (SEM) of polymeric capsules indicated the potential for a longer shelf-life, which promotes the restoration of activity in bacterial cells… More >