Ying Liu^1,3,*, Jingye Chen^1,3, Xiaohao Li^1,3, Shaoxia Yang¹, Hanqiao Hu^1,3, Yingbin Xue^2,3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 975-987, 2022, DOI:10.32604/phyton.2022.019057

Abstract In order to investigate the effects of Manganese (Mn) toxicity stress on the growth and gene expression at the seedling stage of soybean, soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO₄) and excess Mn concentration (100 μmol/L MnSO₄) by the method of hydroponic culture in this study. When soybean was subjected to Mn toxicity stress, excessive Mn could affect seedling growth, root development, the number of Mn oxide spots in leaves, and the Mn accumulation content in different parts of soybean. With the increase of exogenous Mn concentration and the prolongation of culture time, the shoot and… More >

Zhifeng Xiao, Yukun Jin, Qi Zhang, Abraham Lamboro, Baozhu Dong, Zhuoyu Yang, Piwu Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 349-361, 2022, DOI:10.32604/phyton.2022.017451

Abstract Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean oil. In the synthesis pathway of soybean fatty acids, the FAD2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic acid. In this study, CRISPR/Cas9 gene editing technology was used to regulate FAD2 gene expression. Firstly, the CRISPR/Cas9 single knockout vectors GmFAD2-1B and GmFAD2-2C and double knockout vectors GmFAD2-2A-3 were constructed. Then, the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation, and the mutant plants were obtained. Functional analysis… More >

SULAIMAN A. AL YOUSEF^*

BIOCELL, Vol.45, No.5, pp. 1403-1411, 2021, DOI:10.32604/biocell.2021.015450

Abstract Seeds from soybean collected from different commercial markets were surveyed for seed-borne fungi. Ninetyeight fungal colonies were isolated all over three monthly isolations constituting twenty-two fungal species. The isolated fungi were belonging to the genera: Alternaria, Aspergillus, Cephalosporium, Chaetomium, Cladosporium, Colletotrichum, Curvularia, Fusarium, Macrophomina, Mucor, Penicillium, Rhizoctonia, Rhizopus, Sclerotium, Stemphylium, and Verticillium. Assay of the antifungal activity of four ethanolic extracts of clove, cinnamon, garlic, and mint was carried out against the most common fungal species (Aspergillus flavus, A. niger, Fusarium solani, F. oxysporium, and F. moniliforme), which significantly reduced the growth of tested fungi. Clove extract recorded the highest… More >

Kapil Gupta¹, Sujit Kumar Mishra², Shubhra Gupta³, Saurabh Pandey⁴, Jogeswar Panigrahi^5,*, Shabir Hussain Wani^6,*

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1339-1362, 2021, DOI:10.32604/phyton.2021.015239

Abstract Soybean (Glycine max (L.) Merr) is an agro-economic crop growing across the world to cater nutrition for both human and animal feed due to the high oil and protein content in its edible seeds. The genes and QTLs associated with important agronomic traits in this crop have already been identified and validated for soybean cyst nematode (SCN), Phytophthora root and stem rot, Pythium root rot and aphid resistance, seed quality, nutrient values, and also employed for genetic improvement in soybean. In the last decade, micro RNAs (miRNAs) have been considered the effector molecules, so the detection and characterization of novel… More >

Shuo Qu¹, Yaolei Jiao², Lamboro Abraham², Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1177-1192, 2021, DOI:10.32604/phyton.2021.015206

Abstract The fatty acid dehydrogenase gene plays an important role in regulating the oleic acid content in soybean. Genome-wide association study screened out soybean oleic acid related gene Gm15G117700. A fragment size of 693bp was obtained by PCR amplification of the gene and, it was connected by seamless cloning technology to the pMD18T cloning vector. Based on the gene sequence cloned, bioinformatic analysis of gene protein was performed. The overexpression vector of Gm15G117700 and the CRISPR/Cas9 gene editing vector were constructed. The positive plants were obtained by Agrobacterium-mediated transformation of soybean cotyledon nodes and T₂ plants were identified by conventional PCR,… More >

LI PAN¹, JIAWEI LIU¹, MOHAMMED HAMDY FAROUK^2,*, GUIXIN QIN^1,*, NAN BAO¹, YUAN ZHAO¹, HUI SUN¹

BIOCELL, Vol.45, No.3, pp. 451-459, 2021, DOI:10.32604/biocell.2021.014289

Abstract Soybean agglutinin (SBA) is an important anti-nutritional factor in soybean. SBA can induce animal growth inhibition, cause pathological changes of intestinal tissue, and decrease in the immune system functioning. Recently, a great deal of research has been done on the effects of SBA on cell morphology, division, apoptosis, autophagy, as well as the correlated signal transduction pathway. This review mainly covers the chemical and biological characteristics of SBA, describes the multifaceted aspects of SBA anti-nutritional functions, and highlights the possible cellular and molecular mechanism of anti-nutritional effects of SBA. This review has important implications for the prevention and treatment of… More >

Hesham F. Alharby¹, Mirza Hasanuzzaman^2,*, Hassan S. Al-Zahrani¹, Khalid Rehman Hakeem¹

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 373-388, 2021, DOI:10.32604/phyton.2021.013657

Abstract The mechanism of selenium (Se)-induced salt tolerance was studied in moderately sensitive soybean (Glycine max L.) plants. To execute this view, soybean plants were imposed with salt stress (EC 6 dS m⁻¹ ) applying NaCl. In other treatments, Se (0, 25, 50 and 75 µM Na₂SeO₄) was sprayed as co-application with that level of salt stress. Plant height, stem diameter, leaf area, SPAD value decreased noticeably under salt stress. Altered proline (Pro) level, together with decreased leaf relative water content (RWC) was observed in salt-affected plants. Salt stress resulted in brutal oxidative damage and increased the content of H₂O₂, MDA… More >

Siyi Fu^1,#, Tao Yun^2,#, Dexuan Ma¹, Bingsong Zheng¹, Dean Jiang³, Yi He^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 99-110, 2021, DOI:10.32604/phyton.2021.013262

Abstract The chloroplast NAD(P)H dehydrogenase (NDH) complex, as one of the most important photosynthesis protein complexes in thylakoid membrane, is involved in photosystem I (PSI) cyclic electron transport (CEF). Under abiotic environmental stress, the photosynthetic apparatus is susceptible to the damage caused by the strong light illumination. However, the enhancement of NDHdependent CEF could facilitate the alleviation of the damage to the photosynthetic apparatus. The NdhB subunit encoded by chloroplast genome is one of most important subunits of NDH complex and consists of 510 amino acids. Here, according to cloning ndhB from Melrose (cultivated soybean), ACC547 (wild salt-tolerant soybean), S113-6 and… More >

Xiao Luo^1,*, Hongying Chu², Mengqi Liu¹

Journal of Renewable Materials, Vol.8, No.10, pp. 1295-1304, 2020, DOI:10.32604/jrm.2020.011026

Abstract Herein, epoxidized soybean oil methyl ester (ESOM) plasticizer was synthesized for the preparation of plasticized poly(vinyl chloride) (PVC) films by the alcoholysis and epoxidation. The chemical structure of ESOM was investigated by infrared spectrum and ¹H nuclear magnetic resonance. The effect of content of ESOM and petroleum based plasticizer di-2-ethylhexyl phthalate (DEHP) on the performance of plasticized PVC films was studied. The result showed that substituting DEHP with ESOM can improve the thermal stability of plasticized PVC films. When the weight ratio of ESOM and PVC is fixed at 1:2, plasticized PVC film presents higher elongation at break (350.8% vs.… More >

Kun Li, Xiaona Li, Jing Luo, Jingjing Li, Qiang Gao^*, Jianzhang Li^*

Journal of Renewable Materials, Vol.5, Suppl.1, pp. 31-38, 2017, DOI:10.7569/JRM.2017.634131

Abstract The objective of this study was to use soybean meal and 1,4-butanediol diglycidyl ether (BDDE) to develop a highly water-resistant, soy-based bioadhesive for plywood fabrication. The physical properties and performance characteristics of the resulting adhesive, including solid content, viscosity, water resistance, crystallinity, fracture morphology, thermal behavior, and cracks, were evaluated. The proposed adhesive was compared against the traditional soy adhesive with polyamidoamine-epichlorohydrin (PAE). Results showed that adding 8 g of BDDE into the adhesive formulation improved the solid content up to 32.83% and reduced the viscosity to 27340 mPa·s. The wet shear strength of plywood bonded with the adhesive was… More >